Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tissue section cutting method for sea water left eye floundre and right eye founder fertilized egg

A technology for tissue slicing and fertilized eggs, which is applied in the preparation of test samples, instruments, teaching models, etc., to achieve the effect of avoiding slicing failure

Inactive Publication Date: 2007-12-05
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the inadequacy that the slicing method of fertilized eggs of common organisms and freshwater fish cannot be applied to the slicing of seawater flounder and flounder eggs, the present invention has established a whole set of Material Fixation, Handling and Tissue Sectioning Methods for Saltwater Flounder Roe Slicing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue section cutting method for sea water left eye floundre and right eye founder fertilized egg
  • Tissue section cutting method for sea water left eye floundre and right eye founder fertilized egg

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Flounder fertilized egg tissue section

[0026] Step 1: Put the flounder fertilized eggs of different developmental stages into 50 times the volume of Bouin's fixative for 10 hours, wash with 70% ethanol for several times (wash away the fixative) and store.

[0027] Step 2: After washing the preserved eggs with 70% ethanol, use a dissection needle to puncture the egg membrane in the direction of the plant pole.

[0028] Step 3: Place it on a glass slide, drip hot-melt agar, and adjust the position and orientation of the eggs under a dissecting microscope.

[0029] Step 4: The embedded agar block is dehydrated by 70%, 80%, 90%, 95%, 100% ethanol as a tissue block, and the treatment time of each group is 30min. A small amount of eosin was added to 95% ethanol to stain the oocytes red.

[0030] Step 5: Put it into terpineol for transparent treatment, and the treatment time is 4 hours.

[0031] Step 6: Put it into a mixed solution of terpineol and hot-melt par...

Embodiment 2

[0035] Example 2: Turbot fertilized egg tissue section

[0036] Step 1: Put the turbot fertilized eggs of different developmental stages in 50 times the volume of Bouin's fixative for 12 hours, wash with 70% ethanol for several times (wash away the fixative) and store.

[0037] Step 2: After washing the preserved eggs with 70% ethanol, use a dissection needle to puncture the egg membrane in the direction of the plant pole.

[0038] Step 3: Place it on a glass slide, drip hot melt agar, and adjust the position and direction of the eggs under a dissecting microscope.

[0039] Step 4: The embedded agar block was dehydrated by 70%, 80%, 90%, 95%, 100% ethanol as a tissue block, and the treatment time for each group was 1 hour. A small amount of eosin was added to 95% ethanol to stain the oocytes red.

[0040] Step 5: Put the dehydrated agar blocks into terpineol for transparent treatment, and the treatment time is 6 hours.

[0041] Step 6: Put it into a mixed solution of terpin...

Embodiment 3

[0045] Example 3: Flounder fertilized egg tissue section

[0046] Step 1: Put flounder fertilized eggs of different developmental stages in 100 times the volume of Bouin's fixative for 6 hours, wash with 70% ethanol for several times (wash away the fixative) and store.

[0047] Step 2: After washing the preserved eggs with 70% ethanol, use a dissection needle to puncture the egg membrane in the direction of the plant pole.

[0048] Step 3: Place it on a glass slide, drip hot melt agar, and adjust the position and direction of the eggs under a dissecting microscope.

[0049] Step 4: The embedded agar block is dehydrated by 70%, 80%, 90%, 95%, 100% ethanol as a tissue block, and the treatment time of each group is 45min. A small amount of eosin was added to 95% ethanol to stain the oocytes red.

[0050] Step 5: Put the dehydrated agar blocks into terpineol for transparent treatment, and the treatment time is 12 hours.

[0051] Step 6: Put it into a mixed solution of terpineol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to microscopic tissue section technology, and establishes one effective tissue section method for the fertilized egg of sea water left eyed founder and right-eyed founder based on the features of the fertilized egg of sea water left eyed founder and right-eyed founder. The fertilized egg is polylecithal pelagic egg with high egg membrane toughness and narrow perivitelline space. The tissue section method includes the following steps: 1. fixing the polylecithal pelagic egg with Bouiní»s fixing fluid and optimizing treatment period; 2. pricking the egg membrane with dissecting needle in the vegetative pole direction to peel off the egg membrane; and 3. directly embedded to fix embryo with hot agar. The method is simple and effective, and may be also applied in the tissue section of other sea water fish egg.

Description

technical field [0001] The invention relates to a microscopic tissue slicing technology, in particular to a set of embryo positioning and embedding continuous slicing method suitable for the fertilized eggs of sea flounder and flounder. technical background [0002] In the existing reports on fish eggs, the materials used are all freshwater fish eggs, such as grass carp and crucian carp, while tissue sections of marine fish eggs have not been reported yet. For freshwater fish eggs, they were fixed with Bouin's fixative or Smith's fixative, then removed from the membrane, dehydrated in an ethanol gradient according to the common biological tissue section method, and transparently treated with xylene, and then made into paraffin-embedded sections. [0003] However, the tissue section of flounder roe by this method is easy to be chopped, and a complete section cannot be obtained. The main reason is that flounder and flounder eggs are different from most other freshwater fish e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/06G09B23/28
Inventor 孙威尤锋张培军徐永立
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com