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Two phase Roe's culture medium and preparation method thereof

A technology similar to Roche culture medium and Roche culture medium, applied in the field of medical testing, can solve the problems of time-consuming, inability to meet clinical diagnosis and treatment and disease prevention, low positive rate, etc., to promote rapid growth, facilitate rapid preliminary identification, The effect of improving the positive rate of culture

Inactive Publication Date: 2008-06-04
胡忠义
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This culture medium takes a long time (usually 4-8 weeks), and the positive rate is not high (usually 10%-30%), which is far from meeting the needs of clinical diagnosis and treatment and disease prevention.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: The Roche medium in the present invention is the modified Roche medium commonly used at home and abroad at present, and 4-6ml of 0.1% chromogenic agent TTC solution is added (or not added) to 1000ml of the modified Roche medium; liquid medium It consists of basal medium, growth promoters and bacteriostatic agents.

[0034] The composition of the basal medium is as follows: copper sulfate 0.001 g, zinc sulfate 0.001 g, calcium chloride 0.001 g, magnesium sulfate 0.01 g, oleic acid 0.01 g, ferric ammonium citrate 0.05 g, sodium citrate 0.1 g, ammonium sulfate 0.2 g, sodium pyruvate 0.5 g, Tween-80 0.5 g, asparagine 1 g, potassium dihydrogen phosphate 1 g, sodium dihydrogen phosphate 2 g, neutral glycerin 8 ml, distilled water 1000 ml. The preparation method is as follows: firstly dissolving the above-mentioned components in double distilled water, then sterilizing in high pressure at 115°C-121°C for 10-15 minutes, cooling, and storing in a 4°C refrigerator a...

Embodiment 2

[0039] The composition of the basal medium is as follows: copper sulfate 0.0015 g, zinc sulfate 0.0015 g, calcium chloride 0.0016 g, magnesium sulfate 0.018 g, oleic acid 0.016 g, ferric ammonium citrate 0.07 g, sodium citrate 0.15 g, ammonium sulfate 0.3 g, sodium pyruvate 0.7 g, Tween-80 0.7 g, asparagine 1.3 g, potassium dihydrogen phosphate 1.5 g, sodium dihydrogen phosphate 3 g, neutral glycerin 10 ml, distilled water 1000 ml. The preparation method is as follows: firstly dissolving the above-mentioned components in double distilled water, then sterilizing in high pressure at 115°C-121°C for 10-15 minutes, cooling, and storing in a 4°C refrigerator after sterility test.

[0040] The composition of the growth promoter is as follows: 0.015 g of pyridoxine hydrochloride, 0.015 g of biotin, 0.15 g of coenzyme A, 0.15 g of adenosine triphosphate, 0.15 g of catalase, 0.15 g of α-naphthalene acetic acid, 1.5 g of peptone, glucose, 15 g gram, bovine serum albumin V5 factor 150 gr...

Embodiment 3

[0042] Example 3: The composition of the basal medium is as follows: copper sulfate 0.002 g, zinc sulfate 0.003 g, calcium chloride 0.002 g, magnesium sulfate 0.02 g, oleic acid 0.02 g, ferric ammonium citrate 0.1 g, sodium citrate 0.2 g g, ammonium sulfate 0.4 g, sodium pyruvate 1 g, Tween-80 1 g, asparagine 2 g, potassium dihydrogen phosphate 2 g, sodium dihydrogen phosphate 4 g, neutral glycerin 12 ml, distilled water 1000 ml. The preparation method is as follows: firstly dissolving the above-mentioned components in double distilled water, then sterilizing in high pressure at 115°C-121°C for 10-15 minutes, cooling, and storing in a 4°C refrigerator after sterility test.

[0043] The composition of the growth promoter is as follows: 0.02 g of pyridoxine hydrochloride, 0.02 g of biotin, 0.2 g of coenzyme A, 0.2 g of adenosine triphosphate, 0.2 g of catalase, 0.2 g of α-naphthalene acetic acid, 2 g of peptone, 20 g of glucose, Bovine Serum Albumin V5 Factor 200g. The preparat...

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Abstract

The present invention discloses a preparation method of a new type incubation medium, belonging to the medical inspection field. The method can promote growth of mycobacteria. The incubation medium comprises a solid part and a liquid part: the solid part is the modified Roche's incubation medium that is generally used at home and abroad and the liquid part consists of basic incubation medium, growth promoter and bacteria inhibitor. The incubation medium can not only promote growth of mycobacteria, but also inhibit other bacteria. The growing mycobacteria can form violet red bacteria colony on the solid inclined plane of incubation medium, which is beneficial to elementary appraisal. The incubation medium can shorten incubation time obviously, increase incubation positive rate considerably, very suitable for fast inspection of mycobacteria for the hospitals and the sanitation and anti-epidemic institutions.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a novel culture medium capable of promoting the rapid growth of mycobacteria and a preparation method thereof. Background of the Invention [0002] Mycobacteria are a group of bacteria widely distributed in nature, some of which can cause disease in humans and animals. In recent years, more and more diseases caused by mycobacterial infection have been reported at home and abroad. In particular, secondary infections in immunocompromised patients and outbreaks of nosocomial infections have increased significantly. For the diagnosis of infectious diseases caused by mycobacteria, the diagnosis can be confirmed as long as the mycobacteria are cultured from the sample to be tested. [0003] At present, the modified Roche medium is widely used at home and abroad to isolate and culture mycobacteria (see Chinese Medical Association's Code of Practice for Clinical Tec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
Inventor 胡忠义
Owner 胡忠义
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