Supercharge Your Innovation With Domain-Expert AI Agents!

Process for chromatographic purifying corn SOD thereof

A chromatographic purification, corn technology, applied in biochemical equipment and methods, enzymes, enzymes, etc., to achieve the effects of mild separation conditions, large adsorption capacity, and simple equipment

Inactive Publication Date: 2008-07-02
SHENYANG AGRI UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the purification of corn SOD by metal chelation affinity chromatography at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Take 500 kg of corn kernels, wash them with tap water, soak them in a constant temperature incubator at 28°C-30°C for 24 hours, germinate them for 24 hours, wash them with distilled water, add 1000 liters of potassium dihydrogen phosphate-dihydrogen phosphate at a concentration of 0.05 Potassium extraction buffer is fully ground with a colloid mill, centrifuged at 4000 rpm, and the supernatant is the crude extract of the enzyme. The SOD crude extract was first saturated with 40% ammonium sulfate for 0.5 hours, centrifuged at 8000 rpm, saturated with 90% ammonium sulfate for 2 hours, then centrifuged at 8000 rpm for 20 minutes, redissolved the precipitate, and dialyzed with distilled water 12 hours. The dialysate is centrifuged at 8000 rpm for 20 minutes, and the supernatant is collected, which is the SOD concentrate, and the SOD concentrate is the corn SOD crude enzyme solution.

[0020] 20 g of metal chelate affinity chromatography medium (Chealating Sepharose Fast Fl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to metal chelating compatible medium and its chromatography purification corn SOD method. Its features are that compatible medium components and their weight proportion are as follows: metal chelating compatible medium 5, copper sulfate 0.2, sodium phosphate 2, sodium chloride 5.5, and water 85; it process centrifuge, salting out, dialysis, chromatography, and elution to make core SOD reach electrophoresis pure. The invention has the advantages of large adsorption quantity, gentle separating condition, strong commonality, and specificity. It can simplify pheron purifying technology to make enzyme cost reduce 30%. The formed corn SOD has single component, high recovery rate and purification multiple that are 29.4% and 307. It is high efficiency and fast purifying method, and fit for large-scale industrialization production. The formed corn SOD has strong heat endurance. And its pH application range is very broad. It can be applied to medicine industry.

Description

technical field [0001] The invention relates to an enzyme separation and purification method, in particular to a method for purifying corn SOD by metal chelation affinity chromatography. Background technique [0002] Superoxide dismutase (SOD) is the only enzyme that can scavenge superoxide anion free radicals in organisms, and it is a promising medicinal enzyme. The content of SOD in corn is very rich, and it is one of the three major crops in the world. Using corn as raw material and using bioengineering technology to prepare SOD preparations can increase the added value of this crop by more than 20 times, and the economic and social benefits are very considerable. At present, the large-scale production process of corn SOD complex enzyme is relatively mature, but the problem of its purification has not been solved, which has become an obstacle restricting its use as a medicinal enzyme. The separation and purification of pharmaceutical proteins are generally carried out by...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/08
Inventor 陈红漫樊金娟马镝陶思源陈鹏
Owner SHENYANG AGRI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More