Process for chromatographic purifying corn SOD thereof
A chromatographic purification, corn technology, applied in biochemical equipment and methods, enzymes, enzymes, etc., to achieve the effects of mild separation conditions, large adsorption capacity, and simple equipment
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[0019] Take 500 kg of corn kernels, wash them with tap water, soak them in a constant temperature incubator at 28°C-30°C for 24 hours, germinate them for 24 hours, wash them with distilled water, add 1000 liters of potassium dihydrogen phosphate-dihydrogen phosphate at a concentration of 0.05 Potassium extraction buffer is fully ground with a colloid mill, centrifuged at 4000 rpm, and the supernatant is the crude extract of the enzyme. The SOD crude extract was first saturated with 40% ammonium sulfate for 0.5 hours, centrifuged at 8000 rpm, saturated with 90% ammonium sulfate for 2 hours, then centrifuged at 8000 rpm for 20 minutes, redissolved the precipitate, and dialyzed with distilled water 12 hours. The dialysate is centrifuged at 8000 rpm for 20 minutes, and the supernatant is collected, which is the SOD concentrate, and the SOD concentrate is the corn SOD crude enzyme solution.
[0020] 20 g of metal chelate affinity chromatography medium (Chealating Sepharose Fast Fl...
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