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Cyclic maltosyl maltose, cyclic maltosyl maltose synthase, method of producing the same and use thereof

A maltose and cyclic technology, applied in the field of cyclic maltosyl maltose and cyclic maltosyl maltose synthetase and their production and application, can solve the problems of strong reactivity, small molecules, browning, etc.

Inactive Publication Date: 2008-10-01
HAYASHIBARA BIOCHEMICAL LAB INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is known that sugars with a large value usually have small molecules, low viscosity, and strong sweetness, but they are highly reactive, and are prone to aminocarbonyl reactions with substances with amino groups such as amino acids or proteins, resulting in browning, odor, and poor quality. The nature of deterioration

Method used

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  • Cyclic maltosyl maltose, cyclic maltosyl maltose synthase, method of producing the same and use thereof
  • Cyclic maltosyl maltose, cyclic maltosyl maltose synthase, method of producing the same and use thereof
  • Cyclic maltosyl maltose, cyclic maltosyl maltose synthase, method of producing the same and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0302] Arthrobacter globosa M6 (FERM BP-8448) was seed cultured according to the method in Experiment 3. Then, about 20 L of partially decomposed starch (trade name Pindex #100, manufactured by Matsutani Chemical Industry Co., Ltd.) 3.0 w / v% and soybean peptide (trade name Hiniut SMS, produced by Fuji) were added to a fermenter with a capacity of 30 L. Oil Co., Ltd.) 3.6w / v%, dipotassium hydrogenphosphate 0.1w / v%, sodium dihydrogenphosphate dihydrate 0.06w / v%, magnesium sulfate heptahydrate 0.05w / v%, calcium carbonate 0.3w / The liquid culture medium composed of v% and water was heat-sterilized, cooled, and after reaching a temperature of 27°C, inoculated with 1v / v% of the seed culture solution, maintained at a temperature of 27°C, pH 5.5 to 8.0, and carried out aerated culture for 96 hours. After the cultivation, use SF membrane to perform sterilizing filtration, recover about 18 L of culture filtrate, then concentrate the filtrate with UF membrane, and recover about 1 L of co...

Embodiment 2

[0304] Make potato starch milk with a concentration of about 1% by mass starch milk, then add calcium chloride so that the final concentration is 1mM, adjust to pH 6.0, heat at 95°C for about 20 minutes to gelatinize, and then cool to about 40°C , according to the ratio of 0.26ml (about 1 unit) per 1 gram of starch solids, add the concentrated enzyme solution containing cyclic maltosyl maltose synthase obtained by the method of Example 1, and react at pH 6.0 and temperature 40°C for 48 Hour. Heat the reaction solution to 95°C, keep it for 30 minutes and then cool it down. The filtrate obtained after filtration is decolorized with activated carbon according to the conventional method, and desalted, purified and concentrated by H-type and OH-type ion exchange resins. The yield was about 90%, and the syrup containing cyclic maltosyl maltose with a density|concentration of 65 mass % was obtained. This product contains 31.4% by mass of cyclic maltose-based maltose, 2.2% by mass of...

Embodiment 3

[0306] Tapioca starch is made into starch milk with a concentration of about 1% by mass, then calcium carbonate is added according to a final concentration of 0.1% by mass, adjusted to pH6.0, and then α-amylase ( Manufactured by Nobo Corporation under the trade name "Tamamil 60L", reacted at 95°C for 10 minutes, then autoclaved at 120°C for 20 minutes, and then rapidly cooled to about 40°C to obtain a liquefied solution with a DE of about 3. The liquefaction solution is added according to 1 gram of starch solids 0.26ml (about 1 unit) with the concentrated solution containing cyclic maltosyl maltose synthase obtained by the method of Example 1 and isoamylase ( Hayashibara Biochemical Research Institute Co., Ltd.), reacted for 48 hours at pH 6.0 and 40°C. The reaction solution was heated to 95°C and kept for 30 minutes, then cooled, and the filtered filtrate was used in a conventional method. Activated carbon decolorizes, purifies after desalination by H type and OH type ion exc...

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Abstract

An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo { 6)-+--D-glucopyranosyl-(1 4)-+--D-glucopyranosyl-(1 6)-+--D-glucopyranosyl-(1 4)-+--D-glucopyranosyl-(1 }, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.

Description

technical field [0001] The present invention relates to a ring {→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl- (1→4)-α-D-Glucopyranosyl-(1→} cyclic maltosyl maltose (hereinafter, sometimes simply referred to as “cyclic maltosyl maltose” in this specification) and cyclic maltose Maltose-based maltose synthases and their production methods and uses thereof, DNA encoding the enzymes and recombinant DNA containing the DNA, specifically, cyclic maltose-based maltose and cyclic maltose-based maltose synthases and production methods thereof, Microorganism producing cyclic maltosyl maltose synthetase, DNA encoding the enzyme, recombinant DNA and transformant containing the DNA, production method and production method of cyclic maltosyl maltose using the enzyme, and cyclic maltosyl maltose containing composition. Background technique [0002] Examples of sugars containing glucose as constituent sugars include amylose, starch dextrin, maltodextrin, maltool...

Claims

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Application Information

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IPC IPC(8): C07H3/06A23L1/236A23L1/30A61K8/00A61K8/73A61Q11/00A61K47/36C12N1/20C12N9/26C12P19/18C12P19/24C12R1/06A23L27/30
Inventor 向井和久渡边光西本友之久保田伦夫福田惠温三宅俊雄
Owner HAYASHIBARA BIOCHEMICAL LAB INC
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