Method of organizing polymer porous support material for engineering and its preparation device

A porous scaffold and tissue engineering technology, applied in the field of preparation of polymer porous bodies, can solve the problems of porogen residue, donor source, immune rejection, etc., and achieve the effect of simple manufacturing method

Inactive Publication Date: 2008-11-12
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional treatment methods include drug therapy, tissue and organ transplantation, artificial substitutes and mechanical device therapy, etc., but these methods have their obvious shortcomings
Tissue and organ transplantation includes three types: autologous transplantation, allogeneic transplantation and xenogeneic organ transplantation, but each has its own disadvantages: autologous transplantation usually does not have the problem of immune rejection, but it repairs pathology or defects at the expense of part of the normal tissue of the human body Tissues will not only increase the pain of patients, but some organs are unique and cannot be transplanted automatically; the biggest problem with allogeneic transplantation is the source of donors; xenogeneic organ transplantation not only has immune rejection, but also involves ethical issues
Although the fiber bonding technology can produce a highly porous scaffold that is connected and suitable for tissue regeneration, it has low strength and is easy to deform when used in bone tissue engineering scaffolds; the scaffold pore size obtained by phase separation / freeze drying technology is small, and in There are still limitations in the application in the field of tissue engineering; the gas foaming method is easy to produce closed-cell structure; the porosity of the scaffold obtained by the rapid prototyping technology is low; when the solution casting / particle leaching technology is used, the filtered out water-soluble particles will be obvious. Increase the time to prepare the scaffold, otherwise there will be porogen residues

Method used

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  • Method of organizing polymer porous support material for engineering and its preparation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Put the ice particle porogen 5 with a particle size in the range of 300-400 μm through a standard sieve into the cylindrical mold 3, and gently tap the wall of the cylinder with a wooden hammer or a nylon rod to make the ice particles tightly packed;

[0019] 2) Prepare polylactic acid (PLA) / chloroform solution at a ratio of 1:10, and pre-cool at -20°C;

[0020] 3) Inject the pre-cooled polylactic acid (PLA) / chloroform solution into the upper space of the compression net 4 in the cylindrical mold 3, vacuum through the vent hole 11, and the solution percolates into the pore-forming area under a pressure of 0.002-0.02 MPa. The gap between agent 5 is then frozen and formed in liquid nitrogen to obtain a prefabricated block containing polymer, solvent and ice;

[0021] 4) The prefabricated block obtained above is freeze-dried in a self-made freeze-drying device at -55~61°C, removes the solvent, then slowly raises the temperature, and keeps it below 0°C for an appropriate...

Embodiment 2

[0023] 1) Put the ice particle porogen 5 with a particle size in the range of 800-900 μm through a standard sieve into the cylindrical mold 3, and gently tap the wall of the cylinder with a wooden hammer or nylon rod to make the ice particles tightly packed;

[0024] 2) Prepare polylactic acid (PLA) / chloroform solution at a ratio of 1:8, and pre-cool at -20°C;

[0025] 3) Inject the pre-cooled polylactic acid (PLA) / chloroform solution into the upper space of the compression net 4 in the cylindrical mold 3. After the cover 1 is installed, the high-pressure gas is passed through the vent hole 12, and the pressure is 0.5-1.5MPa. The solution seeps into the gap of the porogen 5 under a pressure of 100%, and then freezes and shapes in liquid nitrogen to obtain a prefabricated block containing a polymer, a solvent, and ice;

[0026] 4) The prefabricated blocks obtained above are freeze-dried at - (55-61)°C in a self-made freeze-drying device to remove the solvent, then slowly raise ...

Embodiment 3

[0028] 1) Put the ice particle porogen 5 with a particle size in the range of 800-900 μm through a standard sieve into the cylindrical mold 3, and gently tap the wall of the cylinder with a wooden hammer or nylon rod to make the ice particles tightly packed;

[0029] 2) Prepare poly-3-hydroxybutyrate (PHB)-chloroform solution at a ratio of 1:5, and pre-cool at -20°C;

[0030] 3) Inject the pre-cooled poly 3-hydroxybutyrate (PHB) / chloroform solution into the upper space of the compression net 4 in the cylindrical mold 3, and after the cover 1 is installed, vacuumize through the air hole 11, at 0.005-0.04 Under the pressure of MPa, the solution seeps into the gap of the porogen 5, and then freezes and shapes in liquid nitrogen to obtain a prefabricated block containing a polymer, a solvent, and ice;

[0031] 4) The prefabricated block obtained above is freeze-dried in a self-made freeze-drying device at -55~61°C, removes the solvent, then slowly raises the temperature, and keeps...

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Abstract

A porous polymer scaffold material for tissue engineering is prepared through putting the ice particles in a mould, negative-pressure osmosis of pre-cooled polymer solution into the gaps between ice particles, freezing to become a prefabricated block, freeze drying for removing solvent and vacuum drying for removing ice particles.

Description

technical field [0001] The invention relates to a preparation technology of a polymer porous body, in particular to a preparation method and a preparation device of a porous polymer scaffold material for tissue engineering. Background technique [0002] The loss or dysfunction of tissues and organs is one of the major hazards to human health and the leading cause of human disease and death. Traditional treatment methods include drug therapy, tissue and organ transplantation, artificial substitutes and mechanical device therapy, etc., but these methods all have their obvious shortcomings. Tissue and organ transplantation includes three types: autologous transplantation, allogeneic transplantation and xenogeneic organ transplantation, but each has its own disadvantages: autologous transplantation usually does not have the problem of immune rejection, but it repairs pathology or defects at the expense of part of the normal tissue of the human body Tissues will not only increas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/14A61L27/34A61L27/56
Inventor 董寅生林萍华蒲跃朴孙浩郭超
Owner SOUTHEAST UNIV
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