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Process for prodn of L-Thr

An amino acid and glutamic acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that the relationship between the production of L-amino acid is not very clear, and achieve the effect of improving the ability

Inactive Publication Date: 2009-08-19
AJINOMOTO CO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the relationship between the Entner-Dorlov pathway and L-amino acid production is still not well understood

Method used

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  • Process for prodn of L-Thr
  • Process for prodn of L-Thr

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0117] The present invention will be explained in more detail below with reference to examples.

[0118] Gene cloning of enzymes involved in the Entner-Dorlov pathway

[0119] The edd gene and the eda gene have been cloned from bacteria such as Escherichia coli and Zymomonas mobilis, and they encode two enzymes EDD and EDA involved in the Entner-Dorlov pathway, respectively. Enterobacter agglomerans belongs to the genus Enterobacter in classification, but it is generally considered to be closely related to Escherichia coli. Another study found that the genes of Escherichia coli can be expressed in Enterobacter agglomerans. Therefore, it was decided to clone the edd and eda genes from E. coli.

[0120] The edd and eda genes constitute an operon in Escherichia coli (J.Bacteriol., 174 (14): 4638-46, July 1992), so edd-F (SEQ ID NO: 1) and eda-R (SEQ ID NO: 2) Such a pair of primers simultaneously amplifies two genes and a DNA fragment including the two genes by means of PCR. ...

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Abstract

In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.

Description

field of invention [0001] The present invention relates to a method for producing L-amino acid and a bacterial strain used in the production process. Specifically, the present invention relates to bacteria having enhanced ability to produce L-amino acid and a method for producing L-amino acid using the bacteria. Background technique [0002] Traditionally, L-amino acids (such as L-glutamic acid) have been produced by fermentation of short coryneform bacteria belonging to the genus Brevibacterium, Corynebacterium or Microbacterium (“Amino acid fermentation”, GakkaiShuppan Center, pp. , 195-215, 1986). Further development with Bacillus, Streptomyces, Penicillin (U.S. Patent No. 3,220,929), Pseudomonas, Arthrobacter, Serratia, Aerobacter, Candida (U.S. Patent No. 3,563,857) , Escherichia (Japanese Patent Laid-open Publication (kokai) No. 5-244970) and other bacteria produce L-amino acids. Later, bacteria of the genus Enterobacter (EP1078989A2) Klebsiella, Erwinia and Pantoea...

Claims

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Application Information

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IPC IPC(8): C12P13/04C12P13/14C12R1/19C12N15/09C12N1/21C12N9/88C12R1/01
CPCC12P13/04C12N9/88C12P13/14
Inventor 原吉彦泉井裕浅野贵弘渡边保之中松亘
Owner AJINOMOTO CO INC