Antheraea pernyi silk fibrion biomedicinal material and the preparation method

A technology of tussah silk fibroin and biomedical materials, applied in the field of biomedical materials, can solve the problems that cannot meet the requirements of three-dimensional scaffold shaping and mechanical properties

Inactive Publication Date: 2009-10-21
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the molecular weight is too small, it cannot meet the requirements for the preparation of three-dimensional scaffolds for biomedical materials and mechanical properties.

Method used

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  • Antheraea pernyi silk fibrion biomedicinal material and the preparation method
  • Antheraea pernyi silk fibrion biomedicinal material and the preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Put 0.6 kg of leftover tussah silk (a by-product of a silk reeling factory) into 10 liters of 0.05% sodium carbonate aqueous solution, boil for 0.5 hours, repeat the treatment three times, and remove the sericin around the fibroin to obtain pure tussah silk white;

[0023] 2. Put the pure tussah silk fibroin after natural drying into a ternary solution with a weight ratio of lithium thiocyanate: mercaptoethanol: urea of ​​80:5:5 by weight, at a temperature of 60°C Dissolved under high temperature for 20 hours, purified by filtration and ultrafiltration, ultrasonic defoaming for 1 hour, and dried to make pure tussah silk fibroin;

[0024] 3. Put the pure tussah silk fibroin protein in formic acid solution, dissolve it for 4 hours at a temperature of 30°C, the final concentration of the solution is 10%, filter, purify, and defoam to make spinning raw materials;

[0025] 4. Using the electrospinning method, the distance from the receiving plate to the spinneret is 10cm...

Embodiment 2

[0028] 1. Put 1 kg of cocoon layer into 20 liters of neutral soap solution with a concentration of 0.5%, boil for 2 hours, remove the sericin around the silk fibroin, wash it thoroughly with hot water to obtain pure tussah silk fibroin;

[0029] 2. Place the dried pure tussah silk fibroin in a ternary solution of lithium thiocyanate: mercaptoethanol: urea = 85:5:10 by weight, and treat it for 50 hours at a temperature of 40°C. Dissolved into tussah silk fibroin protein solution, purified by filtration, dialysis, and dried into pure tussah silk fibroin protein;

[0030] 3. Put the pure tussah silk fibroin and polylactic acid with a mass ratio of 90:10 in a formic acid solution with a concentration of 80%, dissolve them for 2 hours at a temperature of 50°C, purify by filtration, and defoam Make the spinning stock solution that the final concentration of the solution is 10%;

[0031] 4. Electrospinning is used to spray the above spinning stock solution according to conventional ...

Embodiment 3

[0034] 1, prepare pure tussah silk fibroin by the method for step 1 in embodiment one;

[0035] 2. Treat the dried pure tussah silk fibroin in a solution of lithium thiocyanate: mercaptoethanol: urea = 98:1:1 by weight at a temperature of 80°C for 5 hours, and obtain tussah silkworm after dissolving Silk fibroin solution, filtered, purified, and dried into pure tussah silk fibroin;

[0036] 3. Put pure tussah silk fibroin and gelatin in a mass ratio of 80:20 in hexafluoroisopropanol solution, dissolve at 20°C for 150 hours, filter, purify, and defoam The final concentration of the solution is 5% spinning raw material;

[0037] 4. Using the electrospinning method, the distance from the receiving plate to the spinneret is 15cm, and the voltage is 15KV to spray the above spinning stock solution to obtain a three-dimensional non-woven fabric composed of fibers with a tussah silk fibroin content of 80% and a gelatin content of 20%. Biomedical materials with spun mesh structure. ...

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Abstract

The invention discloses a biomedical engineering material and a preparation method thereof, in particular to a three-dimensional tissue repair material and a preparation method thereof, which belong to the technical field of biomedical materials. It uses natural tussah silk as raw material. After the tussah silk fibroin is dissolved, the electrostatic spinning method is used to obtain a three-dimensional non-woven network structure composed of tussah silk fibroin fibers. The fiber diameter is 50nm-20μm, and the pores between the fibers are 1-500μm. , the thickness of non-woven net is 50nm~20mm; the molecular weight of tussah silk fibroin is 10×104~20×105D. This biomedical engineering material has the characteristics of non-toxic and harmless, good biocompatibility, and excellent cell adhesion and growth performance. It is rich in raw materials and low in cost. It is expected to replace high-cost biomedical materials such as collagen for artificial skin, Repair materials such as tendon, cartilage, and dura mater, especially tissue cell induction materials.

Description

technical field [0001] The invention relates to a biomedical engineering material and a preparation method thereof, in particular to a three-dimensional tissue repair material and a preparation method thereof, which belong to the technical field of biomedical materials. Background technique [0002] Tussah silk contains a large amount of Arg-Gly-Asp (arginine-tyrosine-aspartic acid, RGD) tripeptide sequence, and this sequence has been proved to be beneficial for cell adhesion [Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y.Attachment and growth of fibroblast cells on silkfibroin.Biochem Biophys Res Commun, 1995,208(2):511-516; PierschbacherMD, Ruoslahti E, [J].Nature, 1984, (309):30- 33; Pierschbacher MD, Ruoslahti E. Pro Natl Acad Sci USA, 1984, (81): 5985-5988]. This point was proved in the in vitro cell culture experiment of Sofia [Sofia S, McCarthy MB, Gronowic G, et. al. Biomed Mater Res, 2001, (54): 139-148.]. The above disclosed research content shows that when the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/22A61L31/06
Inventor 左保齐
Owner SUZHOU UNIV
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