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Method for tissue culture quick breeding of sea-buckthorn

A technology of tissue culture rapid propagation and seabuckthorn, applied in the field of plant tissue culture, can solve the problems of slow growth, low survival rate, slow initiation of new shoots, etc., and achieve the effect of high rooting rate

Inactive Publication Date: 2011-06-01
尹伟伦 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because sea buckthorn is a dioecious plant, the offspring propagated by seeds are prone to variation, and the sex of the seedlings is difficult to distinguish, so it can only be used for the construction of ecological forests
And carry out vegetative propagation with seedling cutting, grafting and root tiller, have the shortcomings such as seedling rate is low, speed is slow, be difficult to adapt to the demand of market to nursery stock
With the continuous expansion of the planting area of ​​seabuckthorn, in order to meet the large demand for new strains of seedlings with excellent traits such as drought resistance, cold resistance, salt-alkali resistance, sand burial resistance, disease and insect resistance, high yield, large fruit without thorns, etc., The in vitro propagation technology of seabuckthorn has been widely reported in just over ten years, but its new shoots start slowly, the survival rate is low, and the reproduction coefficient is not high.
On the rapid propagation medium, it can be seen that there are lateral roots on the regenerated plants, which can survive independently after ramets, but grow slowly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Select plump seabuckthorn seeds, sterilize the surface and germinate on the germination medium; the germination medium contains 6.5g / L agar powder and 30g / L white granulated sugar. After the true leaves are fully grown, cut off 1.5-2.0cm shoot tips for proliferation culture, the basic medium is WPM; the additional plant hormone is: 0.3mg / L BA; it also contains 6.5g / L agar powder and 30g / L L white sugar. Cut out healthy single seedlings with a length of 3-4cm for rooting culture, the basic medium is 1 / 4SH; the additional plant hormones are: 0.3mg / L IBA and 0.03mg / L NAA; also contains 1.0g / LAC , 7.0g / L agar and 15g / L white sugar, pH 5.8; the culture temperature is 25±2°C, the light intensity is 1500-2000lx, and the photoperiod is 18h / d. When cultured to 50 days, the multiplication factor, average plant height, rooting rate, average root number and average root length were counted. The results showed that 3.19 adventitious buds were differentiated from each explant on av...

Embodiment 2

[0013]Select plump seabuckthorn seeds, sterilize the surface and germinate on the germination medium; the germination medium contains 6.5g / L agar powder and 30g / L white granulated sugar. After the true leaves are fully grown, cut off 1.5-2cm shoot tips for proliferation culture. The basic medium is WPM; the additional plant hormone is: 0.4mg / L BA; it also contains 6.5g / L agar powder and 30g / L White sugar. Cut out healthy single seedlings with a length of 3-4cm for rooting culture, the basic medium is 1 / 4SH; the additional plant hormones are: 0.4mg / L IBA and 0.04mg / L NAA; also contains 1.0g / LAC , 7.0g / L agar and 15g / L white sugar, pH 5.8; the culture temperature is 25±2°C, the light intensity is 1500-2000lx, and the photoperiod is 18h / d. When cultured to 50 days, the multiplication factor, average plant height, rooting rate, average root number and average root length were counted. The results showed that 4.25 adventitious buds were differentiated from each explant on average...

Embodiment 3

[0015] Select plump seabuckthorn seeds, sterilize the surface and germinate on the germination medium; the germination medium contains 6.5g / L agar powder and 30g / L white granulated sugar. After the true leaves are fully grown, cut off 1.5-2cm shoot tips for proliferation culture. The basic medium is WPM; the additional plant hormone is: 0.5mg / L BA; it also contains 6.5g / L agar powder and 30g / L White sugar. Cut out healthy single seedlings with a length of 3-4cm for rooting culture, the basic medium is 1 / 4SH; the additional plant hormones are: 0.5mg / L IBA and 0.05mg / L NAA; also contains 1.0g / LAC , 7.0g / L agar and 15g / L white sugar, pH 5.8; the culture temperature is 25±2°C, the light intensity is 1500-2000lx, and the photoperiod is 18h / d. When cultured to 50 days, the multiplication factor, average plant height, rooting rate, average root number and average root length were counted. The results showed that 5.39 adventitious buds were differentiated from each explant on averag...

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PUM

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Abstract

A tissue culture method for fast reproducing seabuckthorn, belonging to the field of plant tissue culture, includes such steps as choosing round seeds of seabuckthorn, disinfecting, germinating in germinating culture medium until the euphylla is fully grown, taking its stem tip (1.5-2.0 cm), reproductive culturing in the culture medium WPM containing BA (0.3-0.6 mg / L), taking healthy seedling with a length of 3-4 cm, outdoor rooting culturing in the culture medium 1 / 4 SH containing IBA (0.3-0.6 mg / L), NAA (0.03-0.06 mg / L) and AC of 1.9 g, and transplanting when the length of the root is 3-6 cm. The tissue culture method for fast reproducing seabuckthorn has the advantages of inducing seabuckthorn seedings in test tubes to take reproductive culturing rapidly to generate a plenty of adventitious buds with a 80% survival rate, and providing an efficient way for culturing seabuckthorn seedings on an enlarged scale.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method of seabuckthorn, which belongs to the field of plant tissue culture. Background technique [0002] Seabuckthorn (Hippophae rhamnoides L.) is a perennial deciduous fruit tree, shrub or small tree of the genus Hippophae L. in the family Elaeagnaceae. Its fruit, peel, leaf, bark and its processed products, such as fruit juice, pomace, and fruit oil, contain more than 280 physiologically active substances, which have strong anti-inflammatory, bactericidal, pain-relieving and special effects of promoting tissue regeneration. Many of the ingredients have shown miraculous therapeutic effects in killing and inhibiting tumor cells, anti-radiation, anti-coagulation, lowering blood pressure, preventing vascular embolism, anti-aging, anti-fatigue, enhancing body vitality and immunity, and are known as "Green Gold". [0003] At the same time, the branches and leaves of seabuckthorn are lush, the l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H3/00C12N5/04A01C1/00
Inventor 尹伟伦夏新莉刘翠琼
Owner 尹伟伦