Process of synthesizing gastrodiacin by microorganism cell biological transferring parhydroxy benzyl methylol

Abstract

The present invention discloses microbe cell synthesis process of converting p-hydroxy benzyl alcohol to synthesize gastrodine. The process is one converting reaction of wet cell of rmillaria luteo-virens ZJUQH in 1-10 g / 100ml and 0.22 micron microporous film filtered p-hydroxy benzyl alcohol in 0.1-3.1 mmol / L, in the culture liquid comprising sterilized potato in 2 wt% concentration and glucose in 0.5 concentration, at temperature of 20-25 deg.c and shaking table rotation speed of 50-200 rpm for 1-3 days. Compared with available plant cell conversion process, the microbe cell synthesis process has the advantages of short period, simple operation and easy control.

Description

technical field

[0001] The invention relates to high-density cultivation of plateau rare edible fungi by using bioengineering technology, and using the cultured cells to biosynthesize gastrodin with p-hydroxybenzyl alcohol as a substrate, and belongs to the field of bioengineering and microbial fermentation. Background technique:

[0002] Gastrodin, namely 4-hydroxymethylbenzene-β-D-glucopyranoside or p-hydroxymethylbenzene-β-D-glucopyranoside, is the main active ingredient of the Orchidaceae plant Gastrodia elata Blume , has sedative, anti-convulsant, anti-inflammatory and enhanced immune function. It is widely used clinically for vertigo (Ménière's disease, viral vertigo, vestibular neuronitis, occipital neuralgia, etc.), headache (neurasthenia and neurasthenia syndrome, vascular headache, tension headache, brain trauma syndrome) syndrome, migraine, etc.) and adjuvant treatment of epilepsy.

[0003] Biotransformation is a biochemical reaction that uses plant isolated cel...