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Electrode modified by chloride, oxide enzyme capable of catalyzing organic reaction in high effect

A technology of chloroperoxidase and modified electrodes, which is applied in the field of electrochemical catalysis, can solve the problems of reducing enzyme activity and achieve the effects of highly efficient catalysis of organic reactions, broad application prospects, good chiral selectivity and stability

Inactive Publication Date: 2007-07-25
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is also a very important problem of adding hydrogen peroxide to the reaction system with a constant flow pump, which requires buying an expensive constant flow pump. At the same time, once the amount of hydrogen peroxide added is greater than the amount required for the reaction when the activity of the enzyme is reduced

Method used

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  • Electrode modified by chloride, oxide enzyme capable of catalyzing organic reaction in high effect

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The gold disc electrode is polished on metallographic sandpaper first, and then 0.3 and 0.05μm Al 2 O 3 Powder polishing. Then, it was ultrasonically cleaned with ultrapure water, then washed with 50% aqua regia, taken out, rinsed with water, and then placed in 0.2mg / mL sodium mercaptopropane sulfonate (MPS) in ethanol solution for 16h, 3mg / mL poly 2 Assembled in allyl dimethyl ammonium chloride (PDDA) solution for 30 minutes, 3.95x10 -6 It was assembled in mol / L CPO (pH=5.0) for 10 hours, and then moved to 3mg / mL PDDA for 30 minutes. After rinsing with ultrapure water, a modified electrode with immobilized CPO is prepared, which is referred to as PDDA-CPO-PDDA-MPS / Au electrode, which can be used to catalyze organic chemical reactions.

[0041] Use this electrode as the working electrode, the saturated calomel electrode (SCE) as the reference electrode, and the platinum electrode as the auxiliary electrode. Under anaerobic conditions, perform cyclic voltammetric scanning an...

Embodiment 2

[0043] Take 8mL phosphate buffer solution (pH=2.75) of 0.1mmol / L monochlorodimethanone as the base solution, and use the PDDA-CPO-PDDA-MPS / Au electrode prepared in Example 1 as the working electrode. The mercury electrode (SCE) is the reference electrode, and the platinum electrode is the auxiliary electrode. Saturate with oxygen before the reaction, and maintain an oxygen atmosphere during the reaction. The electrolysis is carried out at a potential of -0.6V. The absorbance change before and after electrolysis was measured with an ultraviolet spectrum analyzer. The result is shown in Figure 2.

[0044] The experimental results of the modified electrode for the catalytic chlorination of monochlorodimethanone are shown in Figure 2. It can be clearly seen that curve a is the absorbance of the monochlorodimethone solution before electrolysis; the modified electrode without fixed CPO is the working electrode after 1h electrolysis in the monochlorodimethone solution, the characteristic U...

Embodiment 3

[0047] Use another polycation polyethyleneimine (PEI) to replace the PDDA in Example 1. The other steps are the same as in Example 1. The CPO modified PEI-CPO-PEI-MPS / Au electrode can also obtain the same Catalytic effect.

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Abstract

A myeloperoxidase modified electrode for effectively catalyzing organic reaction is prepared for utilizing molecular assembling film technique to fix trace amount of myeloperoxidase on electrode under electrostatic action, using obtained myeloperoxidase modified electrode as operation electrode and using chemical means to generate hydrogen peroxide at home position for greatly raising conversion efficiency of organic chemical reaction.

Description

Technical field [0001] The invention relates to the field of electrochemical catalysis, in particular to the preparation of a chloroperoxidase modified electrode capable of catalyzing organic reactions by using molecular assembly membrane technology. Background technique [0002] Generally speaking, if biological enzymes can be used to catalyze organic chemical reactions, less energy is required and the raw materials are less toxic, which meets the requirements of environmental protection. At present, peroxidase (peroxidase) is widely used in catalytic oxidation reactions. It has the advantages of being widely available and easily available. More importantly, it uses hydrogen peroxide and other peroxides as oxygen donors. The oxidation reaction can be completed without the participation of cofactors. However, systems catalyzed by peroxidase are generally carried out in a mixture of peroxidase and raw materials. After such a catalytic system completes the catalytic reaction, it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 吴霞琴陆中庆后文璟李和兴
Owner SHANGHAI NORMAL UNIVERSITY
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