Method of separating and purifying ursolic acid and oleanolic acid

A technology for the separation and purification of oleanolic acid, applied in organic chemistry, steroids, sugar derivatives, etc., can solve the problems of low separation efficiency and complicated separation and purification technology, achieve good chemical stability, and simplify extraction steps , The effect of chemical stability and antimicrobial ability

Inactive Publication Date: 2010-09-29
JIANGSU JIUWU HITECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: in order to overcome the deficiencies such as existing separation and purification technology is complicated and separation efficiency is not high, a kind of process is simple and easy, the separation and purification ursolic acid that separation efficiency is high with the combination of water extraction and alcohol extraction, The Oleanolic Acid Method

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  • Method of separating and purifying ursolic acid and oleanolic acid
  • Method of separating and purifying ursolic acid and oleanolic acid

Examples

Experimental program
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Effect test

experiment example 1

[0027] A) Take 5 g of Kuding tea powder, add 100 mL of pure water as the extract, and extract for 1 hour in a boiling water bath, filter, and use 100 mL of anhydrous ethanol solution as the solvent to extract the two residues in a boiling water bath. Each time, the extract was filtered for 2 hours. The filtrate was clarified with a 500nm ceramic membrane at an operating temperature of 40°C, a membrane surface flow rate of 3m / s, and an operating pressure of 0.18MPa. The concentration of ursolic acid in the supernatant was determined to be 0.428mg. / mL, the concentration of oleanolic acid is 0.149mg / mL, the clarified liquid is concentrated 10 times by a nanofiltration membrane with a molecular weight cut-off of 400, and the concentration of ursolic acid in the concentrated solution is 3.878mg / mL and the concentration of oleanolic acid is 1.246mg / mL;

[0028] B) Inject the concentrated solution into the macroporous adsorption resin chromatography, rinse with ethanol, collect the et...

experiment example 2

[0031] A) Take 3 g of Kuding tea powder, add 100 mL of pure water as the extract, extract in boiling water bath for 1 hour, filter, and use 100 mL of anhydrous ethanol solution as solvent for the filter residue to extract three times in boiling water bath , 2 hours each time, the extract is filtered. The filtrate is clarified with a 500nm ceramic membrane at an operating temperature of 40°C, a membrane surface flow rate of 2.5m / s and an operating pressure of 0.15MPa. The concentration of ursolic acid in the clarified liquid is 0.286mg. / mL, the concentration of oleanolic acid is 0.101mg / mL, and the clear liquid is concentrated 10 times by a nanofiltration membrane with a molecular weight cut-off of 300 to obtain a concentration of ursolic acid of 2.437 mg / mL and a concentration of oleanolic acid of 0.923 mg. / mL;

[0032] B) Inject the concentrated solution into the macroporous adsorption resin chromatography, rinse with ethanol, collect the ethanol eluate, and then concentrate a...

experiment example 3

[0035] A) Take 5 g of Kuding tea powder, add 100 mL of pure water as the extract, extract in boiling water bath for 1 hour, filter, and use 100 mL of anhydrous ethanol solution as solvent for the filter residue to extract once in boiling water bath , 2 hours each time, the extract is filtered. The filtrate is clarified with a 500nm ceramic membrane at an operating temperature of 40°C, a membrane surface flow rate of 2m / s and an operating pressure of 0.1MPa. The concentration of ursolic acid in the clarified liquid is detected to be 0.147mg / mL, the concentration of oleanolic acid is 0.191mg / mL, and the clarified liquid is concentrated 10 times by a nanofiltration membrane with a molecular weight cut-off of 500. The concentration of ursolic acid in the concentrated solution is 1.025mg / mL and the concentration of oleanolic acid is 1.306mg / mL. mL;

[0036] B) Inject the concentrated solution into the macroporous adsorption resin chromatography, rinse with ethanol, collect the ethano...

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Abstract

The invention discloses an extracting method of urson and oleanolic acid from bitter leaves, which comprises the following steps: adopting bitter leaves from Hainan as raw material; extracting through pure water; filtering; refluxing filter slag to extract through alcohol; adopting inorganic film filtering method to remove macromolecular impurity in the extract; hyperfiltering; condensing the penetration liquid through nanofiltering; adsorbing and eluting through macromolecular resin chromatographic column; condensing eluent to crystallize; obtaining rough product of total triterpenic acid; washing the rough product; dissolving in the alcohol; alkalining; acidifying; washing again; recrystallizing; obtaining the total triterpenic acid; dissolving the total triterpenic acid in the carbinolto boil; filtering; condensing filtrate; evolving; obtaining the monomer product of oleanolic acid; washing the sediment; dissolving the crystal through alcohol; obtaining the monomer product of urson; hyperfiltering and clarifying filtrate of bitter leaves; nanofiltering; condensing; adsorbing through polyamide resin selectively; eluting; condensing the eluent; obtaining the monomer of tea polyphenol with certain purity.

Description

Technical field [0001] The invention belongs to the technical field of drug separation. Specifically, it relates to a method for extracting and purifying its main active components ursolic acid and oleanolic acid from Kuding tea, and at the same time obtaining a certain purity tea polyphenol by-product. Background technique [0002] Kuding tea is a kind of traditional botanical herbal tea in our country. It has been drinking for more than 2000 years in our country. Modern medical research shows that Kuding tea has cardiovascular effect, antioxidant effect, blood lipid lowering effect and blood sugar lowering effect. , Immune function enhancement, anti-stress, anti-fatigue effect, antibacterial effect, health care and medicinal function of trace elements, antagonism of bronchial smooth muscle contraction, toxicological effect and many other aspects have good curative effect, so it has better Good development prospects. [0003] The composition of Kudingcha is more complicated. In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07D311/58C07H17/06C07D311/22
Inventor 邢卫红徐南平王珏
Owner JIANGSU JIUWU HITECH
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