Micro-fluidic chip and application thereof
A microfluidic chip and substrate technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of false positive test results, non-specific interference, long detection time, etc., and achieve simple manufacturing, simple chip structure, simple form effect
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Embodiment 1
[0031] Chip Design and Fabrication
[0032] (1) Fabricate a microfluidic chip according to the structural design shown in Figure 1.
[0033] On the glass substrate, a 1-3mm separation buffer pool 1, a sample waste liquid pool 2, a sample pool to be tested 3, a separation waste liquid pool 4, and a luminescent substrate sample pool 5 were respectively drilled with a diamond drill; the pools were separated by a wide 100 μm and 30 μm deep (weak acid glass etching solution BOE solution, obtained by wet etching for 1 hour) are connected; the length of the straight-through separation channel 8 from the sample pool 3 to the separation waste pool 4 is 4 cm; The sample pool 3 and the separation channel 8 are connected by the sampling channel 7 of the sample to be tested, and the intersection point where the sample injection channel 7 of the sample to be tested communicates with the separation channel 8 is the intersection 10 of the microfluidic chip; The window 6 is located on the sep...
Embodiment 2
[0037] Chip design and fabrication are the same as in Example 1.
[0038] Taking carcinoembryonic antigen (CEA), a marker of colorectal cancer as an example, the tumor marker has a molecular weight of about 180KD. First, the serum containing CEA was reacted with mAb-ALP (incubated at 37° C. for 30 min), and then the reacted solution was taken for separation. 3.75mM borax solution, 20mM NaCl, 0.01% Tween 20 (pH=9.68) were selected as the buffer system for separation. The injection electric field is 300V / cm, the separation electric field is 400V / cm, and the injection electric field of the chemiluminescence substrate is 400V / cm. The sampling method based on "time" is adopted: open the electrode of the sample cell to be tested and the electrode of the separation waste liquid cell, and at the same time suspend the electrode of the sample waste liquid cell and the electrode of the separation buffer cell, inject the sample for 5 seconds, and the sample to be tested will start from t...
Embodiment 3
[0040] Chip design and fabrication are the same as in Example 1.
[0041] Firstly, the CEA-containing serum and AFP serum were respectively reacted with their corresponding mAb-ALP (incubated at 37° C. for 30 min), then the two solutions were mixed, and the mixed solution was taken for separation. 3.75mM borax solution, 20mM NaCl, 0.01% Tween 20 (pH=9.68) were selected as the buffer system for separation. The injection electric field is 300V / cm, the separation electric field is 400V / cm, and the injection electric field of the chemiluminescence substrate is 400V / cm. The sampling method based on "time" is adopted: open the electrode of the sample cell to be tested and the electrode of the separation waste liquid cell, and at the same time suspend the electrode of the sample waste liquid cell and the electrode of the separation buffer cell, inject the sample for 5 seconds, and the sample to be tested will start from the sample to be tested. The sample pool 3 enters the intersect...
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