Gene for controlling paddy tillering and usage

A gene and rice technology, applied in the direction of genetic engineering, plant gene improvement, application, etc., can solve the problems of unclear molecular mechanism of plant tillering, hovering rice production, etc., and achieve fast and high efficiency

Inactive Publication Date: 2007-09-19
HUAZHONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dwarf breeding in the 1950s and 1960s and hybrid rice breeding in the 1970s were two revolutions in increasing rice production, but in recent years the increase i

Method used

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  • Gene for controlling paddy tillering and usage
  • Gene for controlling paddy tillering and usage
  • Gene for controlling paddy tillering and usage

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Example 1: Isolation and cloning of Osa-MIR156e gene

[0043] 1. Create a mutant library (T0 generation)

[0044] The vector pSMR-J18R was donated by CAMBIA (Center for the Application of Molecular Biology to International Agriculture) in Australia. As shown in Figure 1, the vector pSMR-J18R was introduced in the japonica rice variety Zhonghua 11 (Oryza sativa L.subsp.japonica) in a manner mediated by Agrobacterium EHA105. cv. Zhonghua 11) Randomly insert T-DNA (including Enhancer Trap) (Springer PS. Gene Traps: Tools for plant development and genomics. Plant Cell, 2000, 12: 1007-1020) into the genome to create a mutant library. The mutant library was constructed according to the method described by Wu et al. (Wu C et al., Development of enhancer trap lines for functional analysis of the rice genome. Plant J, 2003, 35:418-427). At present, about 100,000 independent transformants have been obtained.

[0045] 2. Field Screening of T1 Generation Tiller Characters

[0046] 3000...

Example Embodiment

[0071] Example 2: Functional verification and application of Osa-MIR156 gene

[0072] 1. Construction of genetic transformation vector

[0073] The vector used is pU1301 constructed in our laboratory. pU1301 is a commonly used plant genetic transformation vector pCAMBIA1301 (Sun et al., 2004, Xa26, a gene conferring resistance to Xanthomonas oryzaepv.oryzae in rice, encoding a LRR receptor kinase-like protein. Plant Journal. 37: 517-527) A modified Agrobacterium-mediated genetic transformation vector carrying a corn ubiquitin promoter with constitutive and overexpression characteristics (Figure 7). The pCAMBIA1301 vector was donated by the Australian CAMBIA Laboratory (Center for the Application of Molecular Biology to International Agriculture). Use PCR method to expand the target gene SEQ.ID.No.1 directly from the rice genome, the total volume of the PCR reaction system is 20μl, rice genomic DNA template 1ul (about 50ng), 1×Taq enzyme reaction buffer, 25mM MgCL 2 1.2ul, 2mM dNTP...

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Abstract

The invention relates to plant gene engineering field, specially relates to a isolation and cloning of Osa-MIR156e gene for controlling rice tillering and application therefor. The Osa-MIR156e gene has a plurality of orthologous genes in rice gene groups in gene family MIR156 same with arabidopsis ath-MIR156 gene. The Osa-MIR156e gene has function of controlling rice tillering, raising or abating expression amount of Osa-MIR156e gene to control plant tillering or branching amount to generate ideal plant type, so the object of increase production is accessed.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. It specifically relates to a gene Osa-MIR156e controlling rice tillering, and also relates to the application of the gene in transgenic rice or transgenic rice seeds. The present invention adopts the method of forward genetics, screens the T-DNA insertion rice mutant library, and clones the rice tiller gene Osa-MIR156e to control it. Co-segregation detection shows that the gene is closely linked with the multi-tiller mutant phenotype, and through Overexpression of the Osa-MIR156e gene resulted in a phenotype of more tillers in normal rice, which confirmed the function of the gene. It also relates to the carrier containing the gene and other members of the gene family with the core sequence of the gene, as well as other DNA fragments containing the core sequence and homologous genes of other species and relates to using the gene or its functional analogue to regulate tillering of plants ab...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82
Inventor 张启发陈志辉吴昌银
Owner HUAZHONG AGRICULTURAL UNIVERSITY
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