98 results about "Homeotic gene" patented technology

The invention relates to the technical field of plant genetic engineering and discloses clone and application of semi-dominant gene qGL3 capable of controlling grain length and grain weight of rice kernel. In the invention, a main-effective QTL (quantitative trait loci) SEQ ID NO:1 which is capable of simultaneously controlling the grain length and grain weight of rice kernel and a semi-dominant allelic gene SEQ ID NO:3 having advantageous function are separated and cloned; the two genes have the capacities of modifying the yield and appearance quality of rice; and at the same time, the two homologous genes of the two genes in rice are cloned and a fact that the two homologous genes have regulation effect on the length of rice kernel as well is proved. Thus, the gene qGL3 and the advantageous allelic gene qGL3-D thereof as well as the two homologous genes of the gene qGL3 all can be applied to crop genetic modification.

The present invention relates to a method, in particular an in vitro method, for pan-cancer diagnostics, comprising identifying the amount and/or proportion of stable regulatory T cells in a patient suspected of having cancer through analyzing the methylation status of at least one CpG position in the gene foxp3 and/or the gene camta1 or orthologous or paralogous genes thereof, wherein an increased amount and/or proportion of stable regulatory T cells in said patient is indicative for an unspecific cancerous disease. In a second aspect thereof, the present invention relates to a method for diagnosing the survival of a cancer patient, comprising identifying the amount and/or proportion of stable regulatory T cells in said cancer patient through analyzing the methylation status of at least one CpG position in the gene foxp3 and/or the gene camta1 or orthologous or paralogous genes thereof, wherein a demethylation in the gene foxp3 and/or the gene camta1 or orthologous or paralogous genes thereof, is indicative of a stable regulatory T cell, and wherein an increased amount and/or proportion of stable regulatory T cells in said cancer patient is indicative for a shorter survival for said cancer patient. Furthermore, the present invention relates to an improved treatment of cancers based on the inventive methods, and a kit for performing the above methods as well as respective uses.

Methods for transforming forage legumes or woody plants with a DNA construct comprising at least one open reading frame encoding for a caffeoyl CoA 3-O-methyltransferase enzyme or a Medicago sativa caffeic acid 3-O-methyltransferase enzyme or a fragment thereof in either a sense or antisense orientation under a lignification-associated tissue specific promoter have been found, resulting in the down-regulation of the corresponding homologous gene either through antisense inhibition or sense suppression, as well as reduced lignin content and modified lignin composition in the transgenic plants. The expression of the caffeoyl CoA 3-O-methyltransferase transgene produces an increased syringyl lignin to guaiacyl lignin ratio in the transformed plant, and greatly improved forage digestibility.

The invention relates to the field of plant genetic engineering and specifically relates to an application of an osa-MIR167a gene for regulating and controlling plant type of paddy rice. The osa-MIR167a gene has several homologous genes in paddy rice genome and belongs to a same gene family MIR167 as an arabidopsis thaliana ath-MIR167 gene. The osa-MIR167a gene has a function of regulating and controlling the plant type of paddy rice and the nucleotide sequence thereof is shown in the sequence table SEQ ID NO:1 while the core sequence formed by transcription and splicing is shown in the sequence table SEQ ID NO:3. An ideal plant type and increased yield are achieved by changing the expression models of the osa-MIR167a gene or other members in the osa-MIR167a gene family through gene engineering and regulating and controlling the tillering angle, the height and the effective tillering number of the plant. The invention discloses the application of the osa-MIR167a gene for regulating and controlling the plant type of paddy rice.

The invention provides a pollen and anther tapetum specific efficient promoter, the nucleotide sequence of which is shown as the sequence table SEQ ID No.1. The promoter sequence is cloned from a maize genome by screening in a maize genome library and can propel a target gene to have specific expression in the anther tapetum and the pollen. The invention comprises a plant gene engineering method for creating male sterileness by using the promoter sequence, namely, the promoter downstream is joined with a heterologous gene or a homologous gene, and a plant expression vector is constructed, a host plant is transformed, the promoter sequence can propel the downstream genes thereof to express the target protein in the anther tapetum and the pollen in a efficient and specific way to realize creating plant male sterileness or modifying the safety of transgenic plants.

The invention relates to key genes, microRNAs and other non-coding RNAs, or a combination thereof used for identifying or regulating cell pluripotency. The invention is characterized in that the key genes, microRNAs, other non-coding RNAs or a combination is highly expressed in the stem cell with complete pluripotency and the expression is obviously repressed or silent in the stem cell without complete pluripotency. The genes, microRNAs and other non-coding RNAs are the genes, microRNAs and other non-coding RNAs positioned in the chromosome imprinting region named as Dlk1-Dio3 on the long arm of mouse chromosome 12 and the genes, microRNAs and other non-coding RNAs in the genome collinearity regions of other mammals, which have 70%-100% of homology. The invention also relates to applications of the genes, microRNAs and other non-coding RNAs, or the combination thereof used for identifying the pluripotency of the stem cell and regulating cell pluripotency, applications in stem cell typing, applications for regulating the cell pluripotency and the pluripotency state and level of the cell, applications in disease treatment, and applications in the drug target development of tumor treatment or the development of antitumor drugs.

The invention relates to a fusion detection method based on homologous genes of differential SNP markers. The fusion detection method utilizes differential SNP signals of two genes to distinguish, bypasses the difference in sequencing depth, and performs consistency comparison on each sequencing reads sequence and the homologous gene sequence by using abnormal inserted fragment length of double-ended reads and the soft clip signal of single-end reads, so as to look for a continuous consistency SNP mark and infer the breakpoint interval. The fusion detection method based on homologous genes ofdifferential SNP markers can obtain the interval where the breakpoint is located, that is, the last site of the first half and the first site of the second half, and the separation distance of the interval depends on the physical distance of the detected two sites, thus avoiding the problem that the conventional structural variation detection method encounters in the detection of repeated sequences.

The invention relates toOmega3- fatty acid dehydrogenase promoter sequence separated from Pichia Pastoris and its application. The invention comprises SEQ ID NO:1. The invention includes the microbiological gene engineering method of changing microbiological cells protein components with the promoter sequence, and comprises: connecting heterogenic or homologous gene to the promoter downstream, transferring into microbiological expression vector, transforming microorganism host for the expression of target protein in its cells to realize quality improvement or medical use. Omega3- fatty acid dehydrogenase promoter sequence separated from Pichia Pastoris can be used in all the transgenic microorganisms.

The invention discloses a malus hupehensis MhWRKY40a gene and applications thereof. The nucleotide sequence of the malus hupehensis MhWRKY40a gene is shown as the SEQ ID NO.1. The MhWRKY40a gene is capable of applying to the production of novel species, which are salt resistant and low temperature resistant, and/or plant variety improvement. A novel MhWRKY40a gene is cloned from malus hupehensis for the first time, and the homology between the gene sequence and the arabidopis thaliana, grape and barley is 50.89%. But the protein tertiary structure of the MhWRKY40a is prominently different from the homologous gene of the model plant arabidopis thaliana; the former has 5 beta-folds, while the latter has 4 beta-folds. The MhWRKY40a transgenetic tobacco has the properties of salt resistance and low-temperature resistance, raises the resistance-associated gene expression, and therefore the fact, which the MhWRKY40a gene has the function of improving the salt resistance and low-temperature resistance properties of the plants, is testified.

According to the present invention, the ability to produce a desired product is significantly improved and the growth rate and the fermentation rate are maintained at excellent levels for yeast upon production of a desired product with the use of yeast

A yeast used as a host is introduced with a foreign gene that encodes an enzyme involved in the production of a desired product and the HAP4 gene that can be constitutively expressed or a homologous gene thereof.

Preferably a yeast mutant is a mutant strain having lowered alcohol productivity than that of a wild-type strain.

The invention discloses a method for batch computing of the evolutionary rate of orthologous genes of a genome. According to the method for batch computing of the evolutionary rate of the orthologous genes of the genome, an InParanoid program for clustering searching of orthologous genes after a paired comparing result is obtained based on Blast, an MUSCLE program based on multi-sequence comparative analysis and a PAML software package yn00 program for evaluating the synonymous and non-synonymous substitution ratio based on comparison between coding protein DNA sequences are comprehensively used, and the methods such as Perl scripting language programming are combined. It is proved through experiments that a method comparing system for batch computing of the evolutionary rate of the orthologous genes of the genome is good in repeat effect on various parameter values for detecting the genome and computing the evolutionary rate of the orthologous genes of the genome, high in speed and capable of easily achieving batch computing, automation and processing.

The invention discloses a gene sequence of Lygus lucorum polygalacturonase (PG) and a method for preparing the Lygus lucorum polygalacturonase. The method comprises the following steps of: extracting total RNA (ribonucleic acid) of Lygus lucorum; designing a primer according to the conserved sequences of the Lygus lucorum polygalacturonase; amplifying by utilizing an RT-PCR (reverse transcriptase-polymerase chain reaction) method to obtain homologous gene sequences of three PGs; obtaining 5' and 3' unknown sequences by utilizing an RACE experiment; and finally, respectively carrying out the recombinant expression, purification and property analysis of the Lygus lucorum polygalacturonase by utilizing an Escherichia coli expression system and a Pichia pastoris expression system. By using the method, the obtaining problem of the gene sequence of the polygalacturonase of the Lygus lucorum is solved firstly, and the recombinant expression problem of the polygalacturonase derived from insects is also solved for the first time. The gene disclosed by the invention has the application prospect in the aspects of food (fruit juice squeezing), oil material extraction, traditional Chinese medicinal material treatment, paper-making industry, oligo-pectin health care products and the like. In addition, an inhibitor aiming at the Lygus lucorum polygalacturonase can be used as a policy for preventing and controlling a piercing-sucking type pest, thereby achieving the prevention and control on the target pest.

The invention relates to a rapid cloning method of plant flowering related gene LFY/FLO homologous fragments and special primers thereof, and relates to the technical field of rapid isolation and cloning of plant homologous genes. The method is characterized in that the used special primer LYF has a nucleotide sequence of 5'-TAYATIAAYAARCCIAARATG-3', and LYR has a nucleotide sequence of 5'-ARIYKIGTIGGIACRTACCA-3'; the primer concentration is 25 muM, and the annealing temperature is 44 DEG C. The method of the invention can clone to obtain homologous fragments of LFY/FLO genes of plants from bryophytes to spermatophytes, and has a wide range of the kinds of applicable plants; the potential problem that a gene fragment is difficult to be amplified due to no expression or low expression of homologous fragments of the LFY/FLO gene when cDNA is used as a template is well avoided; and the method is rapid, simple, economical, and effective.

The invention discloses a nitraria tangutorum CBL-interacting protein kinase 9 (NtCIPK9) gene, expressed protein of the NtCIPK9 gene and application of the NtCIPK9 gene. The nucleotide sequence of the NtCIPK9 gene is shown as the SEQ ID NO.1, and the amino acid sequence of the NtCIPK9 gene is shown as the SEQ ID NO.2. According to the NtCIPK9 gene, a full-length gene related to stress resistance of nitraria tangutorum is cloned in a homological mode by using leaf blade tissue of the nitraria tangutorum on the basis of a part of existing transcriptome data according to the stress resistance characteristic of the nitraria tangutorum, and is named NtCIPK9 according to a homologous gene in arabidopsis. The function of plant salt tolerance of the NtCIPK9 gene is proved through analysis of salt tolerance of an NtCIPK9 gene homozygous arabidopsis plant, and resources are increased for a plant stress resistance gene pool.

The invention belongs to the field of biotechnology, and particularly relates to a SNP molecular marker for detecting lobed-leaf trait of brassica campestris vegetables and applications thereof. The SNP molecular marker is selected from: (a) the 358th locus of a template strand of Bra009510 gene, wherein the rna polymorphism is G or A; and (b) a homologous gene homologous to the Bra009510 in a brassica campestris vegetable variety with an integrifolious trait maintained, wherein the rna polymorphism corresponding to the 358th locus of SEQ ID NO.1 is G, and/or a homologous gene homologous to the Bra009510 in a brassica campestris variety with a lobed-leaf trait, wherein the rna polymorphism corresponding to the 358th locus of the SEQ ID NO.1 is A. The brassica campestris vegetable variety with an integrifolious trait maintained and/or the brassica campestris variety with a lobed-leaf trait are selected from: natural varieties, hybrid varieties and artificial mutagen varieties. The sequence of the template strand of the Bra009510 gene is as shown in the SEQ ID NO.1. The molecular marker provided by the invention and corresponding primers have a selection accuracy of 100% whether in lobed-leaf or integrifolious materials, and can be used for molecular marker-assisted selection breeding.

The invention relates to a method for constructing a B6-Chr1<ZZ2>/Xiao mouse model through replacing the No.1 chromosome based on a ZZ2 mus musculus source. The method comprises the steps as follows: a ZZ2 mus musculus is hybridized with a mouse C57BL/6J, so as to obtain a F1-generation mouse; secondly, the F1-generation mouse is backcrossed with the C57BL/6J, and a F2-generation mouse is obtained; thirdly, a mouse of which the No. 1 chromosome is in a heterozygosis state is screened out through genotyping; fourthly, the F2-generation mouse is backcrossed with the C57BL/6J; fifthly, the same method is adopted for backcross until a 10-generation is screened out, and a mouse group of which the No.1 chromosomes are in the heterozygosis state while the other chromosomes are all pure C57BL/6J genotypes is formed; sixthly, the DNA of the genes of the backcross mouse is identified; finally, when the backcross mouse with the No.1 chromosome in the heterozygosis state of ZZ2 and B6 is subjected to the 10-generation backcross, homologous genes carrying the No.1 chromosome of the ZZ2 mus musculus is bred and introduced into an inbred strain, and the mouse model is obtained through brother-sister selfing and gene identification.