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Moxa leaf polysaccharide and its use

A technology of mugwort leaf polysaccharide and xylose, applied in the field of mugwort leaf polysaccharide and its application in the preparation of medicines or health care products for the treatment or prevention of cancer, can solve the problem of lack of in-depth research on the chemical structure of anticancer active ingredients, undetermined active ingredients, and clinical efficacy Instability and other problems, to achieve the effect of enhancing the body's immune drugs or health products, stable curative effect, and meeting the needs of daily consumption and clinical medication

Inactive Publication Date: 2007-11-07
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Some people use Ye AI injection and Ye AI tablets to treat digestive tract tumors and breast cancer ("Anti-Cancer Chinese Herbal Medicine", 1981, People's Health Publishing House: 144), but the clinical efficacy is not stable because the active ingredients have not been determined.
[0004] In recent years, it has been found that the water extract of Folium Artemisiae Argyi has the effect of inhibiting the proliferation of certain tumor cells (PhytotherapyResearch, 2005, 19, 649), and Chinese patent CN1962698 ("Use of Folium Artemisiae Artifact Polysaccharide Extract") found that the total polysaccharide extract of Folium Artemisiae Artifacts not only has the effect on It has obvious inhibitory effect on tumors in vivo, and also has immune regulation effect, but the chemical structure of anti-cancer active ingredients is still lack of in-depth research

Method used

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  • Moxa leaf polysaccharide and its use
  • Moxa leaf polysaccharide and its use
  • Moxa leaf polysaccharide and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Take 10.0g of total polysaccharides from Artemisia argyi leaves, add 50ml of water to fully dissolve them, put them on the well-balanced ion-exchange chromatography column DEAE-02-cellulose, elute with water and 0.1mol / L NaCl solution in sequence, and track and detect with sulfuric acid-phenol method For polysaccharide components, the same chromatographic peak components were combined, and the eluent was concentrated under reduced pressure at 50°C to 10mL, then put on the GEL 01 column, eluted with water and 0.1mol / L NaCl solution in sequence, and the sulfuric acid-anthraquinone method was followed to detect the polysaccharide components , combined the components of the same chromatographic peak, freeze-dried, and obtained a total of 4.9 g of the polysaccharide fraction AYP01 with an average molecular weight of about 6,800 Da.

[0042] The purity of polysaccharide AYP01 was determined by HPLC, the chromatographic column was DEAE-sepharose, the mobile phase was gradient e...

Embodiment 2

[0054] Take 10.0g of the total polysaccharide AYTP of Artemisia argyi leaves, add 50ml of water to fully dissolve it, put it on the well-balanced ion-exchange chromatography column DEAE-sepharose F.F., elute with water and 0.1mol / L NaCl solution in sequence, and track and detect the polysaccharide with sulfuric acid-phenol method Components, the same chromatographic peak components were combined, the eluent was concentrated under reduced pressure at 50°C to 20mL, put on a Sephadex G-25 column, eluted with water and 0.1mol / L NaCl solution in sequence, and the sulfuric acid-anthraquinone method was followed to detect the polysaccharide components , combined the components of the same chromatographic peak, freeze-dried, and obtained a total of 5.1 g of the polysaccharide fraction AYP01 with an average molecular weight of about 6,800 Da.

[0055] Carry out relevant antitumor pharmacodynamics, immune effect, antioxidative effect and toxicology research on the polysaccharide AYP01 of...

Embodiment 3

[0057] The inhibitory effect of AYP01 on HL60 cells was determined by MTT method:

[0058] HL60 cells in the logarithmic growth phase were centrifuged (Institute of Cells, Shanghai Academy of Biological Sciences, Chinese Academy of Sciences), diluted into a cell suspension, and seeded on a 96-well culture plate, 100 μl per well. After the cells were inoculated, 100 μl of drugs (including blank control) of different concentrations were added. Set up 3 replicate wells for each group of concentration, in 5% CO 2 , and cultured at 37°C for a certain period of time. 24 hours after adding the drug, 10 μl of MTT working solution was added to each well with a final concentration of 10%, that is, 500 μg / ml, and incubated for 4 hours under cell culture conditions. Take out the culture plate, centrifuge at 1000 rpm for 5 minutes, carefully suck off the supernatant, add 100 μl of DMSO, place in the incubator and incubate for 5-10 minutes, select the wavelength required for detection on ...

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Abstract

The present invention discloses moxa leaf polysaccharide and its use. The compound has obvious tumor inhibiting effect, capacity of enhancing body's immunological function and powerful antioxidation effect, and may be used for the auxiliary treatment of tumor and taken by persons with weak health. It has reliable curative effect, low cost and no toxic side effect.

Description

technical field [0001] The invention relates to a polysaccharide extracted from leaves of mugwort and its use, in particular to a polysaccharide from leaves of mugwort and its application in preparing medicines or health care products for treating or preventing cancer. Background technique [0002] Folium Artemisia Argyi (Folium Artemisia Argyi) is a traditional Chinese medicine in my country. Its main functions are: dispelling cold and relieving pain, warming meridian and stopping bleeding ("Chinese Pharmacopoeia", 2005, Chemical Industry Press: 120). The chemical components mainly contain volatile oil, adenine, choline, tannins, flavonoids, sterols, terpenes, polysaccharides, trace elements and other organic components ("Chinese Materia Medica", 1999, Shanghai Science and Technology Press: 1864). [0003] Some people use Ye AI injection and Ye AI tablets to treat digestive tract tumors and breast cancer ("Anti-Cancer Chinese Herbal Medicine", 1981, People's Health Publishi...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K36/282A61P35/00A61P37/02A61P39/06
Inventor 蓝闽波何正有郁荣华袁慧慧郭晶张艳红赵红莉王越刘建文
Owner EAST CHINA UNIV OF SCI & TECH
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