Moxa leaf polysaccharide and its use

A technology of mugwort leaf polysaccharide and xylose, applied in the field of mugwort leaf polysaccharide and its application in the preparation of medicines or health care products for the treatment or prevention of cancer, can solve the problem of lack of in-depth research on the chemical structure of anticancer active ingredients, undetermined active ingredients, and clinical efficacy Instability and other problems, to achieve the effect of enhancing the body's immune drugs or health products, stable curative effect, and meeting the needs of daily consumption and clinical medication

Inactive Publication Date: 2007-11-07
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Some people use Ye AI injection and Ye AI tablets to treat digestive tract tumors and breast cancer ("Anti-Cancer Chinese Herbal Medicine", 1981, People's Health Publishing House: 144), but the clinical efficacy is not stable because the active ingredients have not been determined.
[0004] In recent years, it has been found that the water extract of Folium Artemisiae Argyi has the effect of inhibiting the proliferation of cer

Method used

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  • Moxa leaf polysaccharide and its use
  • Moxa leaf polysaccharide and its use
  • Moxa leaf polysaccharide and its use

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0040] Example 1

[0041] Take 10.0g of the total polysaccharide of mugwort leaves, add 50ml of water to fully dissolve it, and apply it to the ion exchange chromatography column DEAE-02- cellulose that has been equilibrated, eluting with water and 0.1mol / L NaCl solution in turn, followed by the sulfuric acid-phenol method. The polysaccharide components are combined with the same chromatographic peak components. The eluent is concentrated under reduced pressure at 50°C to 10mL, and then applied to the GEL 01 column, followed by elution with water and 0.1mol / L NaCl solution. The polysaccharide components are tracked and detected by the sulfuric acid-anthraquinone method , Combine the same chromatographic peak components and freeze-dry to obtain 4.9 g of AYP01 polysaccharide component AYP01 with an average molecular weight of about 6,800Da.

[0042] The purity of Artemisia argyi polysaccharide AYP01 was determined by HPLC, the column was DEAE-sepharose, th...

Example Embodiment

[0053] Example 2

[0054] Take 10.0g of AYTP total polysaccharide from Mugwort leaves, add 50ml of water to fully dissolve it, put it on a well-balanced ion exchange chromatography column DEAE-sepharose FF, eluting with water and 0.1mol / L NaCl solution in turn, and use sulfuric acid-phenol method to track the polysaccharide The components are combined with the same chromatographic peak components. The eluent is concentrated under reduced pressure at 50°C to 20 mL, and applied to a Sephadex G-25 column, eluted with water and 0.1 mol / L NaCl solution in turn, followed by sulfuric acid-anthraquinone method for tracking and detecting polysaccharide components , Combine the same chromatographic peak components and freeze-dry to obtain 5.1 g of the AYP01 polysaccharide component AYP01 with an average molecular weight of about 6,800 Da.

[0055] The anti-tumor pharmacodynamics, immune effect, antioxidant effect and toxicology studies on the wormwood polysacchari...

Example Embodiment

[0056] Example 3

[0057] MTT method to determine the inhibitory effect of mugwort leaf polysaccharide AYP01 on HL60 cells:

[0058] Centrifuge the HL60 cells (Institute of Cell Research, Shanghai Academy of Biological Sciences, Chinese Academy of Sciences) in the logarithmic growth phase, dilute them into a cell suspension, and inoculate them on a 96-well culture plate with 100 μl per well. After cell inoculation, 100 μl of drugs of different concentrations (including blank control) were added. Set 3 replicate holes for each group concentration at 5% CO 2 , Incubate for a certain period of time at 37°C. After adding the drug for 24 hours, add 10 μl of MTT working solution to each well, the final concentration is 10%, that is, 500 μg / ml, and incubate for 4 hours under cell culture conditions. Take out the culture plate, centrifuge at 1000 rpm for 5 minutes, carefully aspirate the supernatant, add 100μl of DMSO, incubate in the incubator for 5-10 minutes,...

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Abstract

The present invention discloses moxa leaf polysaccharide and its use. The compound has obvious tumor inhibiting effect, capacity of enhancing body's immunological function and powerful antioxidation effect, and may be used for the auxiliary treatment of tumor and taken by persons with weak health. It has reliable curative effect, low cost and no toxic side effect.

Description

technical field [0001] The invention relates to a polysaccharide extracted from leaves of mugwort and its use, in particular to a polysaccharide from leaves of mugwort and its application in preparing medicines or health care products for treating or preventing cancer. Background technique [0002] Folium Artemisia Argyi (Folium Artemisia Argyi) is a traditional Chinese medicine in my country. Its main functions are: dispelling cold and relieving pain, warming meridian and stopping bleeding ("Chinese Pharmacopoeia", 2005, Chemical Industry Press: 120). The chemical components mainly contain volatile oil, adenine, choline, tannins, flavonoids, sterols, terpenes, polysaccharides, trace elements and other organic components ("Chinese Materia Medica", 1999, Shanghai Science and Technology Press: 1864). [0003] Some people use Ye AI injection and Ye AI tablets to treat digestive tract tumors and breast cancer ("Anti-Cancer Chinese Herbal Medicine", 1981, People's Health Publishi...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K36/282A61P35/00A61P37/02A61P39/06
Inventor 蓝闽波何正有郁荣华袁慧慧郭晶张艳红赵红莉王越刘建文
Owner EAST CHINA UNIV OF SCI & TECH
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