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Industrial process of producing xylanase with Aspergillus usamii

A technology of xylanase and solid-state fermentation, applied in the field of enzyme engineering, can solve the problems of high production and application cost of xylanase, low activity of xylanase, no xylanase, etc., and achieve low production cost , Simple equipment, environment-friendly effect

Inactive Publication Date: 2007-11-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, whether it is liquid fermentation or solid-state fermentation, the activity of microbial xylanase is low, resulting in high production and application costs of xylanase, which limits the wide application of this enzyme, especially in the feed industry and xylo-oligosaccharide production and other fields; and there is no literature or patent report on the industrial production of xylanase by solid-state fermentation of Aspergillus usami

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Strain: Aspergillus usami E001.

[0030] Solid state fermentation equipment: 30M 3 Solid-state fermenter with automatic temperature control blowing device, mechanical turning device, mechanical water replenishing device and mechanical discharging device, etc. The diameter of the tank is 4.5m, the height of the tank is 2.0m, and 1000kg of base material (calculated as dry material) is actually loaded, and the accumulation thickness of the fermentation medium is 30cm.

[0031] Solid-state fermentation medium: 1000kg solid-state fermentation base material (bagasse 450kg+ bran 450kg+ bean cake powder 100kg), (NH 4 ) 2 SO 4 20kg, KH 2 PO 4 5kg, CaCl 2 0.5kg, MgSO 4 0.5kg, CoCl 2 0.05kg, FeSO 4 ·7H 2 O 0.05kg, ZnCl 2 0.05kg, Tween-80 4.0kg, tap water 1800kg, pH 6.0.

[0032] Solid-state fermentation conditions: add fermentation medium to 6M 3 In a high-pressure cooker, heat it to 121°C with steam, keep it warm for 40 minutes, cool it to 36-38°C, inoculate 3kg...

Embodiment 2

[0035] On the basis of Example 1, in order to facilitate the growth of bacteria, achieve the purpose of shortening the fermentation cycle and controlling the contamination of bacteria, this example adopts a variable temperature control mode in which the solid-state fermentation temperature is high before and then low.

[0036] Embodiment 1 Fermentation medium is placed in 30M after sterilization, cooling, inoculation 3 solid state fermenter. (1) Early stage of fermentation (0-20h): 0-8h, close the air inlet and outlet, and set the temperature controller at 32°C; 8-12h, open 1 / 3 of the air inlet and outlet, and then gradually Open the air inlet and outlet until they are all open, and the temperature is still controlled at 32°C. (2) Middle stage of fermentation (20-48h): Turn the koji once every 20 hours and add 180kg of sterile water at the same time, and lower the set temperature to 31°C; turn the koji once again at 36 hours and add 180kg of sterile water at the same time, S...

Embodiment 3

[0039] Change the ratio of bagasse and bean cake powder in the solid-state fermentation base material, and the other formulas are the same as in Example 2.

[0040] 1000kg base material (bagasse 350kg+bran 450kg+bean cake powder 200kg), (NH 4 ) 2 SO 4 20kg, KH 2 PO 4 5kg, CaCl 2 0.5kg, MgSO 4 0.5kg, CoCl 2 0.05kg, FeSO 4 ·7H 2 O 0.05kg, ZnCl 2 0.05kg, Tween-80 4.0kg, tap water 1800kg, pH 6.0. After the fermentation medium is sterilized, cooled and inoculated, it is placed at 30M 3 In the solid-state fermenter, the fermentation conditions are the same as in Example 2.

[0041] The above-mentioned industrial production test results repeated for 3 batches showed that the average xylanase activity of the mature fermented koji material reached 9616IU / g dry koji after hot air drying at 45-50°C (dried koji water content was about 8%).

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Abstract

The industrial process of producing xylanase with Aspergillus usamii belongs to the field of enzyme engineering technology. In the industrial process of producing xylanase, bagasse, corn cob, bran and other crop leftovers are used as the fermenting base material, and proper amount of inorganic salt, surfactant, water, etc are added for solid fermentation in a 30M3 solid fermenting tank with Aspergillus usamii E001 strain. The mature fermented material is stoved with hot blast at 45-50 deg.c to obtain xylanase product possessing xylanase activity of 7783-9616 IU / g. The present invention provides also the recipe of fermenting culture medium and the fermenting conditions.

Description

technical field [0001] The invention relates to a method for industrially producing xylanase by solid-state fermentation of Aspergillus usami E001 strain using bagasse, corncobs, bran, bean cake powder and other agricultural wastes as base materials, and belongs to the technical field of enzyme engineering. technical background [0002] Xylan (xylans) is a heterogeneous polysaccharide that is composed of multiple xylopyranose residues linked by β-1,4-xylosidic bonds into a main chain, and has a variety of different substituents on its side chains. It is the main component of plant hemicellulose, which is the second most abundant renewable resource in nature after cellulose. [0003] Xylanase (xylanases) is a multi-component enzyme system that acts synergistically in the process of xylan degradation, belonging to hemicellulase, usually refers to β-1,4-D-xylan endocuticle Enzyme (β-1,4-D-endoxylanase, EC3.1.2.8), which can randomly cut the xylosidic bond from the inside of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N1/14C12R1/66
Inventor 邬敏辰李剑芳朱劼周晨妍王瑾李东峰符丹丹邓超
Owner JIANGNAN UNIV
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