Industrial process of producing xylanase with Aspergillus usamii

Abstract

The industrial process of producing xylanase with Aspergillus usamii belongs to the field of enzyme engineering technology. In the industrial process of producing xylanase, bagasse, corn cob, bran and other crop leftovers are used as the fermenting base material, and proper amount of inorganic salt, surfactant, water, etc are added for solid fermentation in a 30M3 solid fermenting tank with Aspergillus usamii E001 strain. The mature fermented material is stoved with hot blast at 45-50 deg.c to obtain xylanase product possessing xylanase activity of 7783-9616 IU / g. The present invention provides also the recipe of fermenting culture medium and the fermenting conditions.

Description

technical field

[0001] The invention relates to a method for industrially producing xylanase by solid-state fermentation of Aspergillus usami E001 strain using bagasse, corncobs, bran, bean cake powder and other agricultural wastes as base materials, and belongs to the technical field of enzyme engineering. technical background

[0002] Xylan (xylans) is a heterogeneous polysaccharide that is composed of multiple xylopyranose residues linked by β-1,4-xylosidic bonds into a main chain, and has a variety of different substituents on its side chains. It is the main component of plant hemicellulose, which is the second most abundant renewable resource in nature after cellulose.

[0003] Xylanase (xylanases) is a multi-component enzyme system that acts synergistically in the process of xylan degradation, belonging to hemicellulase, usually refers to β-1,4-D-xylan endocuticle Enzyme (β-1,4-D-endoxylanase, EC3.1.2.8), which can randomly cut the xylosidic bond from the inside of th...