Molecule substitution label sequencing parallel detection method-oligomictic nucleic acid coding label molecule library micro-sphere array analysis

Abstract

Molecular displacement label sequence parallel inspection - oligonucleo coded label molecular pool micro-ball array analysis includes: using artificial sequential oligo nucleo-labeled DNA coded molecular pool, coating micro-balls with ligands in correspondent pools to form catching micro-balls, caught ligands combined with labeled ciding molecular pools to form labeled micro-balls array pools. Sample molecules competitively combined with micro-balls catching ligands when sample contains molecules same as those in labeled micro-balls array pools, calculating amount of displaced coded molecular sequence computing kinds and mounts of molecules to be tested. It verifies high flux of inspection with good repeatability, high reliability and sensitivity, so that it is suitable to precisely parallel quantitative analysis concerned with cancer-inhibiting genes and cancer genes pools, cell factor pools, etc.

Description

technical field [0001] The technical field that the invention relates to is a microsphere suspension array system for high-throughput parallel molecular detection, also known as a liquid biochip. In particular, it is a parallel detection method for competitive displacement of a library of oligonucleotide-encoded tag molecules bound on the surface of microspheres. Background technique [0002] With the completion of human genome sequencing and the entry into the era of functional proteomics, in the face of an extremely large number of biomolecules that need to be detected simultaneously in batches of samples, high-throughput parallel detection technologies for many indicators will replace traditional molecular-by-molecule detection methods. The concept of biochip (also known as microarray) came into being from computer chips. The essence of the biochip is that a series of addressable recognition molecules fixed at a certain position are arranged in an orderly array on the su...

AI Technical Summary

Problems solved by technology

However, there are still some inherent defects in planar chips: (1) The chip processing in the physical sense is cumbersome, and the efficiency of a large number of industrial production is not high and expensive.

(2) There are certain differences between the chip array sites due to physical processing, resulting in differences between different sites and differences between different chips, resulting in nearly 10% background deviation and poor repeatability

(3) Insufficient binding kinetics between molecules and the surface of the chip array, such as slow mixing of molecular reaction solution, slow diffusion and uneven distribution of reaction solution molecules due to the size of the chip surface

(4) Physical analog signal conversion is prone to distortion

These developing chip technologies are not very practical

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