Anti-interference food bacteria total number quick detection method and reagent

Abstract

The utility model discloses an anti-interference fast detection method and corresponding reagent for total food bacteria amount. First, prepare the food specimen in proper sample solution according to the national standard principle; and add the non-bacteria ATP eliminator to reduce the interferences to the detection from the somatic cell ATP, the dissociated ATP and other impurities; and then carry out ATP luminous detection after orderly adding the bacteria ATP extracting agent and the bioluminescence agent; carry out the ATP standard solution luminous detection at the same time to gain the logarithm relation of the ATP density and the luminous value; at last, combine the luminous value of the food specimen to calculate the ATP density and the total bacteria amount in the sample. The method in the utility model has the advantages that the interferences of the non-bacteria ATP are completely eliminated by chemical agents without the process of bacteria increase or filtration. The method is characterized in convenient operation, rapid detection, high sensitivity, wide detection range and low detection cost; and thus is applicable to rapid detection of the total bacteria amount in food raw material, product and processing device surface as well as in the application of food quality control.

Description

technical field [0001] The invention belongs to the field of microbial detection, relates to food hygiene quality control, in particular to an anti-interference rapid detection method and a reagent for the total number of food bacteria. Background technique [0002] The total number of bacteria is a must-check microbial index in most food hygiene quality, which can be used to measure the degree of food contamination by bacteria. Eating food with a total number of bacteria exceeding the standard may cause harm to human health. However, the existing detection of the total number of bacteria in food mainly relies on the food hygiene microbiological inspection method specified in the national standard GB / T4789-2003. This method needs to culture the microorganisms for more than 24 hours, the operation is cumbersome, the detection cycle is long, and professional skills are required It is far from meeting the needs of food companies and food hygiene quality supervision agencies to ...

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Problems solved by technology

In recent years, many research institutes in China have also started relevant researches. For example, CN1730664 discloses a rapid detection reagent for the total number of bacteria, which needs to be filtered with corresponding filter cuvettes during use, and the operation is complicated; CN1680804 discloses a rapid anti-interference reagent for microorganisms. The detection method needs to be combined with different culture media to enrich the sample with the liquid large sample method or the closest approximation method. The operation is relatively complicated and the detection time is long, so it is not suitable for rapid detection and on-site supervision of food quality.

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