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Method for using ultrasound wave auxiliary enzyme to extract apple seed oil

An apple seed and ultrasonic technology, applied in the field of oil extraction and apple seed oil extraction, can solve the problems of loss of nutrients, low oil yield, short extraction time, etc. active effect

Inactive Publication Date: 2008-07-09
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the ultrasonic assisted extraction method has a short extraction time and good oil quality, but the oil yield is low; the supercritical extraction method has a high oil yield and good quality, but it is not suitable for large-scale production; Its oil has deep color, poor oil quality, low oil yield, and is easy to cause the loss of oil nutrients; the complex enzymatic processing conditions are mild, and high-quality oil can be obtained, but the time is long, and the oil yield needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] The present invention is implemented in the following specific ways:

[0009] a. Apple seed pretreatment

[0010] a.1 Apple seeds are broken and shelled,

[0011] a.2 pass through a 60-mesh sieve to obtain apple seed powder;

[0012] b.Add buffer

[0013] b.1 The buffer solution is prepared by mixing 0.1mol / L citric acid and 0.1mol / L sodium citrate, the pH value is 4-5.5,

[0014] b.2 The mass mixing ratio of buffer solution and apple seed kernel powder is 6:1~10:1,

[0015] b.3 First pour the apple seed kernel powder into the container, then add the buffer solution according to the mass mixing ratio and mix well to form a material-liquid mixture;

[0016] c. heating

[0017] c.1 Keep the temperature constant when the temperature of the feed-liquid mixture is heated to 40°C-60°C;

[0018] d. Add compound enzyme

[0019] d.1 Add cellulase and acid protease to the feed liquid mixture respectively,

[0020] d.1.1 The amount of cellulase added is 0.2% to 0.8% of the...

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Abstract

The invention discloses an extraction method of apple seed oil by using enzyme aided by ultrasonic wave and overcomes the shortcomings of the existing various extraction methods of seed oil. The technical proposal comprises pretreatment of apple seed, adding buffer solution, heating, adding compound enzyme, ultrasonic treatment, killing enzyme, adding petroleum ether, stirring, centrifugal separation, recovery of petroleum ether, vacuum drying and obtaining the apple seed oil. By utilizing the role of hole and high frequency oscillation, on one hand the invention increases the contact number of enzyme, substrate and products in the reaction solution, meanwhile accelerates the release velocity of products, on the other hand enables the conformation and the structure of enzyme molecule to change for promoting the catalytic activity of enzyme, and the extraction rate of the apple seed oil can reach 24 percent.

Description

[0001] The invention relates to oil extraction from oil-containing materials, in particular to a method for extracting apple seed oil, which belongs to the field of food processing. Background technique [0002] my country is now the world's largest producer and exporter of apple juice concentrate. In 2006, the output of apple juice concentrate reached more than 700,000 tons, and the export of apple juice concentrate was 635,000 tons. It is expected that the export will increase to 800,000 tons in 2010. According to statistics, every 1 ton of fruit juice can produce 1.2 tons of pomace, which contains 14.4 kilograms of apple seeds, so the number of apple seeds produced every year is huge. The oil content of apple seeds is about 24.3%, and the content of unsaturated fatty acids (oleic acid and linoleic acid) in seed oil accounts for about 90% of the total fatty acids. Apple seed oil can lower blood pressure and cholesterol, eliminate toxins in the body, and promote collagen produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23D7/02A23L1/28A23L1/29A23L1/212A23L19/00A23L33/00
Inventor 赵武奇仇农学肖旭霖赵晓春
Owner SHAANXI NORMAL UNIV
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