Method for preparing D-mannitol from konjak refined powder

A technology of konjac powder and mannitol, which is applied in the direction of fermentation, etc., can solve the problems of troublesome operation, large environmental pollution, repeated operations, etc., and achieve the effects of short production cycle, less environmental pollution and simplified process

Inactive Publication Date: 2012-03-21
广西南宁化学制药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses a simulated moving bed to separate and purify mannose. Although the yield is high, the equipment investment is large, the separation operation steps are many, and the operation is cumbersome.
The above-mentioned publicly reported processes all have the disadvantages of repeated operations, large equipment investment, low yield, and large environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take by weighing the konjac fine powder 100.0g that contains glucomannan 75%, slowly add the beta-mannanase aqueous solution of 240ml concentration 0.002% (W / W) under stirring at 170 rev / mins, temperature is 45 ℃, The pH was 6.0 and the hydrolysis was 6 hours. After adding, increase the rotation speed to 285 rpm, adjust the pH to 2.1 with 0.5% sulfuric acid, then increase the pressure to 0.15Mpa, keep the temperature at 126°C for 35 minutes, release the pressure, and cool down to 65°C Use 120 mesh filter nets to filter and remove residue, obtain reddish-brown sugar mixture filtrate 300ml, adjust the pH value of the sugar mixture to 6.0 with 5% (V / V) sodium hydroxide solution, decolorize until the solution is light yellow with activated carbon, and then Perform ion exchange and concentrate in vacuo to 45%. High performance liquid chromatography detected that the mannose content in the concentrated mixed sugar solution was 61.83%. Use 1% sodium hydroxide solution to adj...

Embodiment 2

[0035] Take by weighing the konjac powder 120.0g that contains glucomannan 70%, slowly add to the beta-mannanase aqueous solution of 250ml concentration 0.003% (W / W) under stirring at 180 rev / mins, temperature is 50 ℃, The pH is 5.5 and the hydrolysis takes 5 hours. After adding, increase the rotation speed to 270 rpm, adjust the pH to 2.3 with 2.5% (V / V) hydrochloric acid, then increase the pressure to 0.12Mpa, and keep the temperature at 124°C for 45 minutes before releasing the pressure and cooling down Remove the slag by filtering with a 120-mesh filter at 60°C to obtain 350ml of reddish-brown sugar mixture filtrate, adjust the pH value of the sugar mixture to 6.5 with 2.5% (V / V) sodium hydroxide solution, and decolorize it with activated carbon until the solution is light Yellow, then ion exchanged, concentrated in vacuo to 50%. The mannose content in the concentrated sugar mixture liquid was detected to be 64.5% by high performance liquid chromatography. Use 3% sodium ...

Embodiment 3

[0037] Take by weighing the konjac powder 150.0g that contains glucomannan 78%, slowly add to the beta-mannanase aqueous solution of 320ml concentration 0.004% (W / W) under stirring at 150 rev / mins, temperature is 50 ℃, The pH was 5.0 and the hydrolysis took 4 hours. After adding, increase the rotation speed to 260r / min, adjust the pH to 2.5 with 1.0% (V / V) hydrochloric acid, then increase the pressure to 0.1Mpa, and the high temperature to 121°C, keep this state for 50 minutes, release the pressure, and cool down to 50 ℃ with a 120-mesh filter screen to filter and remove the residue to obtain 370ml of reddish-brown sugar mixture filtrate, adjust the pH value of the sugar mixture to 6.8 with 1.0% (V / V) sodium hydroxide solution, and decolorize until the solution is pale yellow with activated carbon. It was then ion-exchanged and concentrated to 50% in vacuo. High performance liquid chromatography detected that the mannose content in the concentrated sugar mixture was 65.1%. U...

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Abstract

The invention relates to a process for preparing D-mannitol, which uses konjaku flour as raw material. The process for preparation comprises following steps: selecting konjaku fine flour whose glucomannan content is more than 70-80%, firstly, using beta-mannase to hydrolyze the konjaku fine flour under 45-50 DEG C, secondly, using mineral acid 1-3%(V / V) as catalyst to hydrolyze oligosaccharide inhydrolysate of the first step and obtain mannose-glucose mixture, obtaining the mixture whose mannose content is 58-70% through refining, hydrogenating, and then generating mannitol from the mannose, wherein the mannitol content in mixed alcohol is more than 60%, D-mannitol product can be obtained through refining again and drying, and the yield of the mannitol is more than 50% of total material. The invention is a method for producing the D-mannitol with simple operation and high yield and the raw material which is adopted is renewable natural resource.

Description

(1) Technical field [0001] The invention relates to a method for preparing D-mannitol in a biochemical industry, in particular to a method for preparing D-mannitol by using konjac powder. (2) Background technology [0002] Konjac Glucomannan (Konjac Glucomannan) is a very rich natural renewable polymer resource after starch and cellulose. Biodegradable, soluble in water, with good physical and chemical properties, it is a good raw material for chemical industry, medicine, food and functional materials. [0003] D-mannitol (D-mannitol) molecular weight 182.17, white needle-like crystal or crystalline powder, odorless, sweet taste. The density is 1.489g / cm3. The melting point is 166-170°C. The boiling point is 290-295°C (15.7×103Pa). Optical rotation +23~+24 degrees, non-deliquescence, easily soluble in hot water, hot ethanol, dilute acid, dilute solution, hardly soluble in cold ethanol, insoluble in ether. Appropriate sweetness, low calories, no toxic side effects, metab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12P19/02
Inventor 韦振雷米运宏邓梁华梁智徐斌元罗鸣冯忠厚韦海涛
Owner 广西南宁化学制药有限责任公司
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