Process for preparing and separating methyl docosapentaenoate and methyl docosahexenoate
A kind of technology of methyl docosahexaenoate and methyl docosahexaenoate, applied in the field of preparation and separation of methyl docosahexaenoate and methyl docosahexaenoate , which can solve the problems of complicated brine shrimp meal process and lower yield, and achieve the effect of short heating time, avoided loss and high yield.
Active Publication Date: 2008-09-17
NANGTONG HAODI ANTICORROSION EQUIP
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The invention relates to a preparation and separation method of docosapentaenoic acid methyl ester and docosahexaenoic acid methyl ester. Microalgae oil is used as raw material to prepare docosapentaenoic acid and docosahexaenoic acid which are mixed with fatty acid to prepare the docosapentaenoic acid methyl ester and the docosahexaenoic acid methyl ester. The separation method of the the docosapentaenoic acid methyl ester and the docosahexaenoic acid methyl ester provided by the method adopts a silver nitrate column method of separating. In the silver nitrate column method, the quality of the methyl ester sample introduction of the mixed fatty acid is 0.5 percent to 10 percent of the quality of filled silver nitrate blangel; elution gradient is n-hexane solution containing acetone the content of which is 0.5 percent to 15 percent; the elution speed is 0.5 to 5ml/min. The preparation and separation method utilizes the silver nitrate column method to obtain the high purity docosapentaenoic acid methyl ester and the high purity docosahexaenoic acid methyl ester, wherein, the content of DPA is raised from 20.32 percent in raw oil to 99.61 percent, the content of DHA is raised from 44.83 percent to 99.02 percent, and the yield rate by using the silver nitrate column method can reach more than 90 percent.
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