HPLC-ESI-MS/MS measuring method for simultaneously detecting 19 kinds of carbostyril medicaments

A HPLC-ESI-MS, quinolones technology, applied in the field of antibiotic drug analysis, achieves the effects of simple operation, simple methodological indicators, and strong detection specificity

Inactive Publication Date: 2008-12-03
JIANGNAN UNIV
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Problems solved by technology

Tissue samples using o

Method used

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  • HPLC-ESI-MS/MS measuring method for simultaneously detecting 19 kinds of carbostyril medicaments
  • HPLC-ESI-MS/MS measuring method for simultaneously detecting 19 kinds of carbostyril medicaments
  • HPLC-ESI-MS/MS measuring method for simultaneously detecting 19 kinds of carbostyril medicaments

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Experimental program
Comparison scheme
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Embodiment Construction

[0046] 1. Experimental part

[0047] 1. Instruments and reagents

[0048]Accela high-performance liquid chromatography (U.S. Finnigan Company), including Autosampler and Accela pump; TSQ QuantumAccess mass spectrometer (U.S. Finnigan Company); BP211D electronic analytical balance (German Sartorius sartorius); adjustable micro-sampler series (Thermo Labsystems); KQ-250DE ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); nitrogen blowing instrument (OrganomationAssociates, Jnc.); WH-861. ); RJ-LD-IIB low-speed desktop centrifuge (Wuxi Ruijiang Analytical Instrument Co., Ltd.); enrofloxacin, ciprofloxacin, norfloxacin and other 19 kinds of quinolones (Sigma Aldrich); others The chemical reagents were all analytically pure.

[0049] 2. Preparation of standard solution

[0050] Weigh an appropriate amount of enrofloxacin, ciprofloxacin, norfloxacin, ofloxacin, flumequine, oxquinic acid, difloxacin, sarafloxacin, sparfloxacin, and danofloxacin , fleroxacin, marboflox...

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Abstract

A detection method for detecting HPLC-ESI-MS/MS of 19 species of quinolone drugs simultaneously belongs to the antibiotic drug analysis technology field. The detection method comprises three steps: sample treatment, liquid-phase separation and mass spectrometric detection, wherein the 19 species of quinolone antibiotics in a tissue fluid is subjected to positive ion MRM scan; norfloxacin-D5 is taken as the internal standard; C18 chromatographic columns are adopted for separation; water-methanol containing formic acid is taken as the mobile phase for gradient elution; and the eluent is subjected to mass spectrometric detection. The detection method is characterized in that the organic solvent extraction method is adopted for carrying out the sample pretreatment according to the practical situation of the sample and the different detection sensitivity requirements; norfloxacin-D5 is taken as the internal standard; the C18 chromatographic columns are adopted for separation; and the water-methanol containing formic acid is taken as the mobile phase for gradient elution. The pretreatment process of the method is rapid and convenient, the detection result is reliable and sensitive, and the method is suitable for the analysis and the detection of the quinolone drug residues in animal food.

Description

technical field [0001] The invention relates to a method for simultaneously detecting multiple quinolone antibiotics in animal food, and more particularly relates to an HPLC-ESI-MS / MS method capable of simultaneously quantitatively determining 19 quinolone antibiotics in chicken and fish. The invention belongs to the technical field of antibiotic drug fractionation. Background technique [0002] Quinolones are a class of synthetic antibiotic drugs, which are chemically related to nalidixic acid. Their mechanism of action is to directly act on the nucleus of bacteria and inhibit the DNA gyrase of bacteria, so that the enzyme cannot introduce nicks on the double strand of DNA and destroy bacteria. Metabolism and reproduction, quickly kill bacteria. Originally used to treat urinary tract infections, these drugs have now been developed to treat systemic infectious diseases, and are commonly used as preventive and therapeutic drugs in animal feeding. In recent years, the residu...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N33/15
Inventor 胥传来李雅丽
Owner JIANGNAN UNIV
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