Sperm nucleoprotein lysate

Abstract

The invention discloses a sperm nuclear protein lysate; every 100ml of distilled water contains 4.844g of trishydroxymethylaminomethane, 11.7g of sodium chloride, 1.86g of dithiothreitol, 8 to 15g of Gleditsia Sinensis Lam as well as 12.32g of ethylenediaminetetraacetic disodiumsalt; the pH value is neutral. The Gleditsia Sinensis Lam extractive contained in the lysate of the invention does not lead the protein to be modified and does not damage the tail part of the sperm. The lysate of the invention can simply, fast and accurately detecte the DNA of the sperm with low cost and high resolution.

Description

technical field [0001] The invention relates to a reagent used in clinical medicine, which is a sperm nucleoprotein lysate. Background technique [0002] At present, many countries in the world are studying the abnormality of DNA integrity caused by the formation of DNA fragments due to the influence of internal and external environments during sperm maturation, resulting in abnormal sperm morphology and function. Since the integrity of DNA in male infertility testing is a routine test of semen and the main indicator for evaluating male infertility, how to detect sperm DNA integrity is one of the important topics in the field of reproductive medicine. The leading detection method in foreign countries is the sperm chromatin diffusion test, its English name is spermchromatin dispersion, referred to as SCD, and its commercial test kit has been widely used. The SCD detection method has many advantages, such as: simple, fast, accurate, high resolution, etc. However, because the ...

AI Technical Summary

Problems solved by technology

The SCD detection method has many advantages, such as: simple, fast, accurate, and high resolution. However, because the kit contains intellectual property rights, the kit is expensive. Due to this limitation, it has not been widely used in my country at present, and this The sperm nucleoprotein lysate in this kit uses ingredients such as polyethylene glycol octylphenyl ether, sodium dodecyl iodate, etc. These ingredients are all chemical reagents, and their peculiar smell, irritation and corrosiveness are relatively large, and they are not Has toxic ingredients, pollutes the environment, etc.

Images