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Bacillus bifidus freeze concentration leaven and method for preparing same

A bifidobacterium, freeze-concentration technology, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of short storage period, difficult cultivation of bifidobacteria, low activity of starter, etc., and achieve long shelf life , Improve labor productivity and product quality, and have high fermentation activity

Inactive Publication Date: 2009-01-07
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Bifidobacterium freeze-concentrated starter and its preparation method, to solve the problems that Bifidobacterium is not easy to cultivate, the starter has low vigor, and the storage period is short.

Method used

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  • Bacillus bifidus freeze concentration leaven and method for preparing same
  • Bacillus bifidus freeze concentration leaven and method for preparing same

Examples

Experimental program
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Effect test

Embodiment 1

[0040] The medium consists of 15g peptone, 10g beef extract, 3g tryptone, 1g yeast extract powder, 15g glucose, K 2 HPO 4 5g, sodium acetate 0.5g, diammonium hydrogen citrate 0.5g, ZnSO 4 ·7H 2 O 0.1g, MgSO 4 ·7H 2O 0.05g, L-cysteine ​​hydrochloride 0.1g, fructooligosaccharide 0.5g, CaCO 3 0.5g, Tween-80 5g and water 1000g. Adjust the pH of the culture medium to 7.5, and sterilize with 0.05Mpa damp heat for 30 minutes before use.

[0041] Before the strain activation, place the Bifidobacterium longum ampoule stored at 4°C at room temperature for 2 hours, then wipe the surface of the ampoule with 75% alcohol cotton ball in a sterile room for disinfection, and open the ampoule , use a sterile pipette to draw 0.2ml of medium into the ampoule bottle, inoculate the bacteria in the ampoule bottle into the medium at an inoculation amount of 5% (volume percentage), culture in a test tube, and cultivate under anaerobic conditions at 37.5°C for 36h After that, the culture was t...

Embodiment 2

[0049] The medium consists of 15g of peptone, 10g of beef extract, 3g of casein peptone, 1g of yeast extract powder, 15g of lactose, 5g of Tween-80, K 2 HPO 4 5g, sodium acetate 0.5g, diammonium hydrogen citrate 0.5g, ZnSO 4 ·7H 2 O 0.1g, MgSO 4 ·7H 2 O 0.05g, L-cysteine ​​hydrochloride 0.1g, fructooligosaccharide 0.5g, CaCO 3 0.5g and water 1000g. Adjust the pH of the culture medium to 6.0, and sterilize with 0.14Mpa damp heat for 15 minutes before use.

[0050] Before the strain activation, place the Bifidobacterium infantis ampoule stored at 4°C at room temperature for 3 hours, then wipe the surface of the ampoule with 75% alcohol cotton ball in a sterile room for disinfection, and open the ampoule , use a sterile pipette to draw 0.3ml of medium into the ampoule, inoculate the bacteria in the ampoule into the medium at an inoculation amount of 3% (volume percentage), culture in test tubes, and cultivate under anaerobic conditions at 42°C After 24 hours, the culture...

Embodiment 3

[0057] The medium consists of 5g of peptone, 5g of beef extract, 10g of casein peptone, 5g of yeast extract powder, 10g of lactose, 1g of Tween-80, K 2 HPO 4 2g, sodium acetate 5g, diammonium hydrogen citrate 0.2g, ZnSO 4 ·7H 2 O0.3g, MgSO 4 ·7H 2 O 0.1g, L-cysteine ​​hydrochloride 0.5g, fructooligosaccharide 2.5g, CaCO 3 1g, and 1000g of water. Adjust the pH of the culture medium to 7.0, and sterilize with 0.1Mpa damp heat for 25 minutes before use.

[0058] Before the strain activation, place the Bifidobacterium adolescentis ampoule stored at 4°C at room temperature for 2.5 hours, then wipe the surface of the ampoule with 75% alcohol cotton ball in a sterile room for disinfection, and open the ampoule bottle, use a sterile pipette to draw 0.3ml of medium into the ampoule bottle, inoculate the bacteria in the ampoule bottle into the medium at an inoculation amount of 2% (volume percentage), and culture in test tubes at 30°C under anaerobic conditions After 48 h of cu...

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Abstract

The invention discloses a bifidobacterium freezing concentration starter and a preparation method thereof. The preparation method can solve the problems of difficult culturing of the bifidobacterium, lower activity of the starter and short preservation period. The preparation method includes: culturing a strain activation tube, culturing the strain of first grade by a triangle bottle, culturing the strain of second grade with a seed pot, culturing in a fermentation pot, concentrically collecting the strain, adding a protective agent, packaging in vacuum and storing in a freezing way. The starter is mainly characterized by high live bacteria content, longer shelf life, less inoculation dosage, being capable of stabilizing each batch of fermented dairy products, preventing the strain from degradation and pollution as well as greatly improving the working production rate and the product quality of the fermented dairy product industry. Compared with the starter prepared by the vacuum freezing drying method, the freezing concentration starter prepared by the invention has the advantages of simple method, less device investment, lower cost and easy realizing.

Description

technical field [0001] The invention relates to a bifidobacteria freeze-concentrated starter and a preparation method thereof. Background technique [0002] Bifidobacteria are normal physiological bacteria in the human body. They colonize the intestinal tract and are the dominant flora in the intestinal tract. The number of bifidobacteria is closely related to human health. Sexual beneficial bacteria. The main physiological functions of bifidobacteria are: 1. biological barrier and biological antagonism; 2. enhancing immunity and anti-tumor effect; 3. nutritional effect; 4. inhibiting the production of endotoxin; With the increase of age, the influence of environmental pollution and the use of antibiotics, the number of bacteria in the human intestinal tract gradually decreases, resulting in the destruction of the micro-ecological balance in the intestinal tract, leading to the occurrence of various diseases and aging. [0003] The most direct way to replenish bifidobacter...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 邵建宁麻和平彭章普赵昊星刘彩云慕婷婷龚伟中
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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