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Effective component of Clematis chinensis and preparation and use thereof

An effective component, the technology of Clematis, is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve problems involving normal cells and side effects

Inactive Publication Date: 2009-01-21
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of Clematis chinensis and preparation and use thereof
  • Effective component of Clematis chinensis and preparation and use thereof
  • Effective component of Clematis chinensis and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] The preparation of the effective component of embodiment 1 Clematis clematis

[0089] Take 250 g of Clematis clematis, crush it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into extract to obtain 8.8g, the extract and silica gel are mixed as a sample, and the normal phase silica gel column is used for separation, at first using sherwood oil and ethyl acetate (50: 1) as mobile phase to obtain eluent I, Then, chloroform and methanol (10:1) were used as the mobile phase to obtain the eluent II, which was concentrated and dried to obtain 1.6 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is an Agient preparative column (Zorbax SB-C 18 ; 21.2mm × 250mm), the mobile phase is water (A) and acetonitrile (B), and the gradient e...

Embodiment 2

[0092] The analysis of embodiment 2 Clematis effective components

[0093] The HPLC-ELSD joint analysis to the effective component of Clematis clematis of embodiment 1

[0094] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glaci...

Embodiment 3

[0097] Embodiment 3 Clematis clematis active ingredient preparation

[0098] Take 0.5 g of the effective components of Clematis in Example 1 and mix them evenly with 10.5 g of polyethylene glycol-6000, heat and melt, and then transfer the material to drip irrigation, and drop the drug solution into liquid paraffin at 6-8°C. Degreasing, made 400 dropping pills.

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Abstract

The invention relates to an effective component of clematis root, a preparation method and application thereof; the preparation process of the effective component of clematis root in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting clematis root; step 2, the extract is processed with chromatographic column chromatography to obtain eluate; step 3, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 35.6-40.2 min is collected to obtain the effective component.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Clematis clematis, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/716A61P35/00A61K125/00
Inventor 贺庆刘雳史强程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD