Method for estimating aptness of royal jelly storage condition

A technology of royal jelly and suitability, which is applied in the field of food quality evaluation, can solve the problems of difficult evaluation, uneven quality of royal jelly, lack of evaluation methods, etc., and achieves the effects of high sensitivity and simple analysis method.

Inactive Publication Date: 2009-02-11
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of corresponding evaluation methods, quality supervision departments and processing and trading companies have so far been unable to effectively supervise and control the production and circulation of royal jelly, and it is difficult to make a true evaluation of the storage conditions and quality experienced by royal jelly, resulting in The quality of royal jelly varies in the market

Method used

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  • Method for estimating aptness of royal jelly storage condition
  • Method for estimating aptness of royal jelly storage condition
  • Method for estimating aptness of royal jelly storage condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Commercially available 26 amino acid standard samples: histidine (His), serine (Ser), arginine (Arg), glycine (Gly), aspartic acid (Asp), glutamic acid (Glu), threonine amino acid (Thr), alanine (Ala), hydroxyl lysine (Hylys), proline (Pro), cysteine ​​(Cys), lysine (Lys), tyrosine (Tyr), Methionine (Met), Valine (Val), Isoleucine (Ile), Leucine (Leu), Phenylalanine (Phe), Taurine (Tau), Gamma-Aminobutyric Acid (GABA) , aminoisobutyric acid (AABA), ornithine (Orn), glutamine (Gln), asparagine (Asn), hydroxyproline (Hypro) and tryptophan (Trp) (sigma company) dissolved in In purified water, the final concentration is 5pmoles / μL (wherein Cys is 2.5pmoles / μL), measured with an ultra-high performance liquid chromatograph to obtain a standard liquid chromatogram, see figure 1 .

[0024] The newly harvested royal jelly (Camellia royal jelly, Zhejiang) was divided into 5 parts, stored at 25°C, and the free amino acids were measured on the day of harvest, 1 month, 3 months, 6...

Embodiment 2

[0028] The newly harvested royal jelly (rape royal jelly, Henan) was divided into 3 parts and stored at -18°C, 4°C and 25°C respectively, and the free amino acids were determined during the storage period of 6 months.

[0029] Weigh 1.0g royal jelly in a 50mL plastic centrifuge tube, accurate to 0.01g. Add 25 mL of 90% ethanol solution, vortex for 2 min, ultrasonically extract for 3 min, centrifuge at 5000 rpm for 10 min, and take the supernatant. Add 25 mL of 90% ethanol solution to the precipitate again, repeat the above steps twice, and combine the supernatant. Evaporate the liquid to dryness with a rotary evaporator at 40°C, dissolve the residue with ultrapure water, and set the volume to 50 mL, which is the analytical sample liquid. After filtering with a 0.22 μm filter membrane (Millipore, MA, USA), it was determined by ultra-high performance liquid chromatography ( image 3 ).

[0030] The results showed that when the storage period was 6 months, the contents of free...

Embodiment 3

[0032] Collect 50 samples of Camellia royal jelly (Zhejiang), Vitex twig royal jelly (Beijing), Acacia royal jelly (Shandong) and Rapeseed royal jelly (Qinghai) respectively, 200 samples in total, and store them at 25°C for 1 month. Then weigh 1.0g of royal jelly in a 50mL plastic centrifuge tube, accurate to 0.01g. Add 25 mL of 90% ethanol solution, vortex for 2 min, ultrasonically extract for 3 min, centrifuge at 5000 rpm for 10 min, and take the supernatant. Add 25 mL of 90% ethanol solution to the precipitate again, repeat the above steps twice, and combine the supernatant. Evaporate the liquid to dryness with a rotary evaporator at 40°C, dissolve the residue with ultrapure water, and set the volume to 50 mL, which is the analytical sample liquid. After filtration with a 0.22 μm filter membrane (Millipore, MA, USA), it was determined by ultra-high performance liquid chromatography.

[0033] The results showed that the free Gln contents of camellia royal jelly (Zhejiang),...

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Abstract

The invention provides an evaluation method for the suitability of the storage condition of royal jelly. The suitability of the storage condition of royal jelly is judged by detecting the content of free Gln in the royal jelly and according to the content of free Gln in the royal jelly, and the quality of the royal jelly can be further judged. The storage condition of the royal jelly can be sensitively judged, so as to provide a basis for the manufacturers and the consumers to accurately judge the quality of the royal jelly, and the present royal jelly quality specification can also be completed, in order to effectively monitor the quality of the royal jelly.

Description

technical field [0001] The invention relates to a food quality evaluation method, in particular to an evaluation method for the suitability of storage conditions of royal jelly. Background technique [0002] Royal jelly is jointly secreted by the hypopharyngeal gland and the mandibular gland on the head of the nursing bee. It is a milky white or light yellow pulp with a sour, astringent, spicy, sweet and slightly special aroma. shape. Royal jelly plays an important role in the hierarchical differentiation of bees. The small larvae hatched from the fertilized eggs laid by the queen bee can develop into complete reproductive organs in only 16 days if they are fed with royal jelly throughout the larval stage. Queen bee; if only feeding royal jelly in the first three days of the larval stage, and then feeding honey and pollen, it takes 21 days and can only develop into worker bees with incomplete reproductive organs. [0003] The composition of royal jelly is very complex, ric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/02G01N33/48G01N30/02
Inventor 吴黎明胡福良薛晓锋赵静李熠周金慧
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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