Sterile water culture method suitable for proteomics research of Arabidopsis thaliana root system

Abstract

The invention discloses an aseptic water culture method applicable to Arabidopsis thaliana root system proteomics research. Conical plastic tissue culture flask and 80 mesh stainless steel sieve are adopted as cultivation container. Hydroponics nutrient solution is injected into cultivation container. Syringe needle and filter are used as breather. The process of seeding and cultivation is completed on aseptic worktable. The method is simple with low cost and can obtain good repeatability. By the method, root system loss and contamination with algae and fungus in open water cultivation system is completely avoided, at the same time, the difficulty of root breathing in aseptic cultivation system is overcome. Large amount of high biomass, high degree of conformity as well as complete and healthy Arabidopsis thaliana root system can be obtained in short period. The method fully meets the strict requirements of proteomics research on plant root material.

Description

1. Technical field [0001] The invention relates to a hydroponic method, in particular to an aseptic hydroponic method suitable for the study of Arabidopsis root system proteomics. 2. Technical Background [0002] As a model plant, Arabidopsis has been widely used in plant proteomics research, but Arabidopsis's few and fragile root systems and low protein content are difficult to meet the requirements of large-scale and high-throughput proteomics research, which makes it difficult to obtain The conventional cultivation method of Arabidopsis thaliana for the purpose of root system has become a major technical obstacle. Usually, soil culture matrix culture, agar matrix culture and hydroponics are used to obtain Arabidopsis root material, but the above-mentioned methods all have their obvious shortcomings and deficiencies: [0003] ①The plant samples cultured on soil culture medium and agar medium can not recover the complete root system on the one hand, but the part lost in th...

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Problems solved by technology

[0003] ① Plant samples cultured on soil culture medium and agar medium cannot recover the complete root system on the one hand, and the part lost in the medium is precisely the young root tips and root hairs with the most active protein synthesis. On the other hand, the roots are attached by agar, which may cause experimental pollution

[0004] ② Hydroponics is carried out under non-sterile open conditions, which cannot avoid the influence of algae and fungi, and the germination is not neat, and the repeatability is low, and the hydroponics method using rock wool blocks and agar blocks as growth support substrates is more difficult. Separation to intact root system, which will negatively affect the experimental results

[0005] ③ The production of containers used in sterile hydroponics reported at home and abroad is relatively complicated and costly, especially ignoring the influence of the hypoxic environment of hydroponics on root protein expression

[0006] Therefore, it is inevitable to bring a lot of trouble and high cost to obtain Arabidopsis thaliana root material by soil culture substrate culture, agar substrate culture and hydroponics method. Therefore, how to establish a high-throughput, high-reproducibility, low-cost Arabidopsis thaliana sterile hydroponics method can obtain a large number of complete and healthy roots of Arabidopsis thaliana in a short period of time, and apply it to root proteomics research, which has become an urgent problem to be solved by scientists and technicians in this field

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