Microbial fertilizer and preparation thereof
A microbial fertilizer and seed technology, which is applied in the direction of organic fertilizers, can solve the problems of unsatisfactory crop yield increase, single function, and limited application methods, so as to inhibit the reproduction of harmful bacteria, have high biological safety, and prevent soil-borne diseases. Effect of disease
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Embodiment 1
[0056] Prepare the strain fermentation broth as follows:
[0057] a preparation of Bacillus cereus, Bacillus megaterium and Bacillus subtilis fermentation broth: Bacillus cereus CMCC63305, Bacillus cereus BS8641, Bacillus cereus AS2015, Bacillus megaterium ATCC10011 (B.megaterium), and Bacillus subtilis K-M (Bacillus subtilis) seed solution was inserted in the sterilized culture medium containing 5% bean sprouts juice, 0.5% glucose, 1.5% peptone, and the balance was water after 32 days in the ratio of 1:1:1:2:2. After 16 hours of aerobic fermentation at °C, the fermentation broths of Bacillus cereus, Bacillus megaterium and Bacillus subtilis were prepared.
[0058] b prepare Pantoea agglomerans fermented liquid: insert Pantoea agglomerans WLL (Pantoea agglomerans) seed liquid into the sterilized weight ratio of soybean flour 1%; corn flour 2%; yeast extract 0.5%; sugar 1%, monosodium glutamate 0.3%; Potassium dihydrogen phosphate 0.1%, Calcium carbonate 1%; Sodium chloride 0....
Embodiment 2
[0062] Prepare the strain fermentation broth as follows:
[0063] a prepare Bacillus cereus, Bacillus megaterium and Bacillus subtilis fermentation broth: Bacillus cereus CMCC63305, Bacillus cereus BS8641, Bacillus cereus AS2015, Bacillus megaterium ATCC10011 (B.megaterium), and Bacillus subtilis K-M (Bacillus subtilis) seed liquids were independently inserted into the sterilized culture medium of 8% bean sprouts juice, 0.3% glucose, 1.05% peptone, and the balance was water, and passed the conditions of pH7.8 at 34°C. After 20 hours of aerobic fermentation, the fermentation broths of Bacillus cereus, Bacillus megaterium and Bacillus subtilis were independently prepared;
[0064] B prepare Pantoea agglomerans fermented liquid: insert Pantoea agglomerans WLL (Pantoea agglomerans) seed liquid into the sterilized weight ratio of soybean flour 2%; corn flour 1.5%; yeast extract 0.3%; white sugar 2%, monosodium glutamate 0.2%; Potassium dihydrogen phosphate 0.2%, Calcium carbonate ...
Embodiment 3
[0068] The fermented liquid of Bacillus cereus prepared in Example 1, Bacillus megaterium, Bacillus subtilis, Bacillus jelly-like, Pantoea agglomerans is mixed according to the following weight ratio:
[0069] Bacillus cereus ATCC63305 5%
[0070] Bacillus cereus BS8641 5%
[0071] Bacillus cereus AS2015 5%
[0072] Bacillus megaterium ATCC10011 10%
[0073] Bacillus subtilis K-M 10%
[0074] Bacillus jelly-like ATCC10013 20%
[0075] Pantoea agglomerans WLL 20%
[0076] Azotobacter rotundum ATCC10003 25%
[0077] Then pack and make microbial fertilizer of the present invention.
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