Tissue culture method of Nerium indicum

Abstract

The invention provides a method for culturing tissue of Nerium indicum cv. Variegata. The method comprises the following steps: an excellent individual plant axillary bud stem segment of bred Nerium indicum cv. Variegata is adopted as explants; the explants are disinfected and then are inoculated in a disinfected glass bottle containing a starting culture medium; the glass bottle is placed under a common fluorescent lamp taken as a light source, and is illuminated for 14 hours per day at a temperature of between 22 and 27 DEG C and at the humidity of between 50 and 65 percent; after 40 days of culture, the explants are differentiated and grow into test-tube bud seedlings; the grown bud seedlings are cut, transferred into a disinfected glass bottle containing a proliferation culture medium, subjected to proliferation culture, continuously proliferated, cut, inoculated in a disinfected glass bottle containing a strong seedling culture medium and subjected to strong seedling culture; after 30 days, basal calluses and partial leaves are removed, and 4 to 5 leaves are left; the bud seedlings are inoculated in a disinfected glass bottle containing a culture medium for the primordium culture of rooting treatment produced roots, subjected to rooting culture for 7 to 12 days, taken out, washed and transplanted into a matrix containing peat and yellow soil with the volume ratio of 1 to1; the bud seedlings root and survive after 25 days; and the rooting rate reaches more than 86 percent.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of oleander. Background technique [0002] Nerium indicum cv. Variegata is an evergreen erect large shrub of the genus Nerium in the family Apocynaceae. Native to Iran, India, and Nepal, it is a commonly used color-leaved tree species widely planted in the provinces and regions south of the Yangtze River in my country. The whole plant is yellow and green, some leaves are all yellow, and some leaves are yellow and green. The plant is 4-5 meters high, has strong sprouting ability and is extremely resistant to pruning. The leaves are leathery, slightly thinner than the original species, the corolla is pink, about 5 cm in diameter, with special aroma, single or double petals, oblong fruit, flowering period from June to October, often planted in parks, courtyards, streets, green spaces, etc. For viewing. In order to keep the original character of the plant, ...

AI Technical Summary

Problems solved by technology

Due to the lack of germplasm resources, it is difficult to obtain a large number of plants in a short peri...