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Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid

A technology for compound extraction of mulberry leaf flavonoids, applied in the direction of alkaloids, alkaloids, drug combinations, etc., can solve the problems of co-production of mulberry leaf flavonoids, polysaccharides and alkaloids, and the composite process and application research of three active substances Lack of basic data and other issues to achieve the effect of improving medicinal utilization and economic added value, easy to scale up industrial production, and high economic value

Inactive Publication Date: 2011-06-01
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the above patents on the extraction process of the effective parts of mulberry leaves are aimed at a single flavonoid or polysaccharide or alkaloid, or two effective parts thereof. Realize the joint production of three medicinal ingredients of mulberry leaf flavonoids, polysaccharides and alkaloids, which greatly wastes precious mulberry leaf resources
[0005] Although the prior art shows that single active substances such as flavonoids, polysaccharides and alkaloids in mulberry leaves have relevant extraction processes, they have not developed mulberry leaf resources in depth from the perspective of comprehensive utilization of medicinal ingredient resources, and have not formed a set of methods for extracting mulberry leaves. The composite process of the three typical active substances in leaves lacks basic data for the application research of compound extraction of flavonoids, polysaccharides and alkaloids in mulberry leaves

Method used

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  • Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid
  • Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid

Examples

Experimental program
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Embodiment 1

[0029] White mulberry leaves are selected as raw materials, washed and dried at 60°C until they become brittle, crushed and sieved (with a sieve diameter of 40-80 mesh) to obtain mulberry leaf powder. Extract the mulberry leaf powder with water at a temperature of 60°C, a solid-to-liquid ratio of 1:10, and a pH of 7, then filter to obtain the mulberry leaf water extract and mulberry leaf residue, and add the mulberry leaf residue to hydrochloric acid-ethanol at a solid-to-liquid ratio of 1:10 -Aqueous solution (containing 1% hydrochloric acid, 40% ethanol, volume fraction), extracting to obtain a solution containing mulberry leaf alkaloids, the content of mulberry leaf flavonoids in the obtained mulberry leaf water extract is 2.5mg / mL, and the content of mulberry leaf polysaccharides is 2.0mg / mL. After the obtained mulberry leaf water extract is adsorbed by a cross-linked polystyrene macroporous adsorption resin column, the resin column is first eluted to colorless with disti...

Embodiment 2

[0031]The mulberry leaf is selected as raw material, washed and dried at 60°C until it becomes brittle, crushed and sieved (with a sieve diameter of 40-80 mesh) to obtain the mulberry leaf powder. Extract the mulberry leaf powder with water at a temperature of 90°C, a solid-to-liquid ratio of 1:50, and a pH of 10, and then filter to obtain the mulberry leaf water extract and mulberry leaf residue. Add the mulberry leaf residue to hydrochloric acid- Ethanol-water solution (containing hydrochloric acid 3%, ethanol 80%, volume fraction), extraction obtains the solution containing mulberry leaf alkaloids, and the content of mulberry leaf flavonoids in the obtained mulberry leaf water extract is 3.2mg / mL, and the content of mulberry leaf polysaccharide is 2.8 mg / mL. After the obtained mulberry leaf water extract is adsorbed by a cross-linked polystyrene macroporous adsorption resin column, the resin column is first eluted to colorless with distilled water to obtain an aqueous solut...

Embodiment 3

[0033] The mulberry leaf is selected as the raw material, washed and dried at 60° C. until it becomes brittle, crushed and sieved (with a sieve diameter of 40 to 80 mesh) to obtain the mulberry leaf powder. Extract the mulberry leaf powder with water at a temperature of 85°C, a solid-to-liquid ratio of 1:40, and a pH of 8, and then filter to obtain the mulberry leaf water extract and mulberry leaf residue. Add the mulberry leaf residue to hydrochloric acid at a solid-to-liquid ratio of 1:30- Ethanol-water solution (containing hydrochloric acid 1.5%, ethanol 65%, volume fraction), extraction obtains the solution containing the total alkaloids of mulberry leaves, the total flavonoid content of mulberry leaves in the obtained mulberry leaf water extract is 2.8mg / mL, and the total polysaccharides of mulberry leaves The content is 2.9mg / mL. After the obtained mulberry leaf water extract is adsorbed by a cross-linked polyacrylate macroporous adsorption resin column, the resin column...

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Abstract

The invention relates to a complex extraction process for mulberry leaf flavonoid, polysaccharide and alkaloid through cogeneration, which is characterized by having the following extraction steps: raw material processing, extraction, and separation. The complex extraction process adopting a complex extraction method for the mulberry leaf flavonoid, the polysaccharide and the alkaloid obtained through the cogeneration has the advantages of mild condition, low energy consumption, small environment pollution; macroporous absorbent resin and chloroform used in the complex extraction process can be reused through regeneration; the economic value of the obtained effective ingredients is very high; the mulberry leaf resource is greatly saved; a new utilization approach of the agricultural product mulberry leaf is opened up; and the medicinal utilization value and the economic added value of the mulberry leaf are improved.

Description

1. Technical field [0001] The invention belongs to the technical field of biological separation, and in particular relates to a composite extraction process for co-producing flavonoids, polysaccharides and alkaloids of mulberry leaves. 2. Background technology [0002] Prior art: Mulberry leaf is the dry leaf of Morus alba L., a plant of the family Moraceae, cold in nature, sweet and bitter in taste, and has the effects of evacuating wind-heat, clearing lung and moistening dryness, clearing liver and improving eyesight, and is one of the commonly used traditional Chinese medicines. It is mainly used for symptoms such as fever, dizziness, headache, cough, and sore throat caused by exogenous wind-heat, and it is also used for symptoms such as red eyes, astringent pain, and excessive tears caused by excess heat in the liver meridian. Mulberry leaves are rich in nutrients, containing 16 kinds of amino acids needed by the human body, and 7 kinds of vitamins also contain nutrients...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/40C08B37/00C07D211/40C07G5/00A61K36/605
Inventor 王俊吴福安赵辉王加力吴倩张金亮
Owner JIANGSU UNIV OF SCI & TECH
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