Apoptosis methods, genes and proteins

A cell and genotype technology, applied in the field of genes and proteins, which can solve problems such as inability to die, lack of "small" cells, and inability to respire functional mitochondria
CN101437845AInactive Publication Date: 2009-05-20MORVUS TECH LTD
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
MORVUS TECH LTD
Publication Date
2009-05-20
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

A W303a Saccharomyces cerevisiae yeast cell which contains a polynucleotide that encodes a functional Bax polypeptide under the control of a galactose- inducible promoter that is integrated at the LEU2 chromosomal locus. A kit of parts comprising the yeast cells and a yeast plasmid vector suitable for transforming a cDNA library into the yeast cells. Use of the yeast cell for screening a cDNA library for a polynucleotide that is or encodes an inhibitor of B ax-mediated apoptosis. Genes and polypeptides that inhibit Bax-mediated apoptosis and which were identified from a human hippocampus cDNA library screened in the yeast cells. A method of combating Bax-mediated apoptosis in a cell using an inhibitor of Bax-mediated apoptosis which was identified from a human hippocampus cDNA library screened in the yeast cells. A method of promoting Bax-mediated apoptosis in a cell using an inhibitor or antagonist of the anti-apoptotic polypeptides identified from a human hippocampus cDNA library screened in the yeast cells.
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Description

technical field

[0001] The present invention relates to the identification of genes and proteins that regulate apoptosis. In particular, the present invention relates to yeast strains useful in methods for identifying modulators of apoptosis, screening methods using said yeast strains, and genes and proteins identified thereby. The invention also relates to medical uses of the identified genes and proteins. Background technique

[0002] Abnormal expression of apoptosis regulatory proteins is often the cause of various diseases such as cancer, rheumatoid arthritis, neurodegeneration and cardiovascular diseases. Numerous lines of evidence strongly suggest that apoptosis contributes to the death of neuronal cells in various neurodegenerations. Such as in stroke, spinal cord trauma (spinal cord trauma), head injury (head injury), spinal muscular atrophy (spinal muscular atrophy, SMA), amyotrophic lateral sclerosis (amyotrophic lateral sclerosis, ALS), Alzheimer's Apoptosis pl...

Claims

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