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Method for preparing ginsenoside Rb<1>

A technology of ginsenoside and ginseng, which is applied in the field of preparation of active ingredients of ginsenoside Rb1, and can solve the problems of no content determination, high cost, and high cost

Inactive Publication Date: 2012-01-04
PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Ginsenoside Rb 1 Many laboratory methods are used to obtain a small amount (mg level to g level) for scientific research, such as preparation of high performance liquid chromatography, silica gel column chromatography, etc., but it is impossible to obtain batches (kg level or more) of ginsenoside Rb 1 It is used for the development and industrial production of finished medicine, so this is ginsenoside Rb 1 Reasons and Difficulties of Difficult Medicine So far
[0005] Application No. 03127664.4 patent document "environmental protection type ginsenoside Rb 1 High yield industrial separation", this document discloses that ginsenoside Rb with a content greater than 95% can be obtained 1 , using silica gel column chromatography, the method is expensive and difficult to industrialized production; another major problem in this document is that there is no method for analysis and detection to show that ginsenoside Rb 1 The content reaches 95%, and its credibility is doubtful; the patent document "Ginsenoside Rb" with application number 03 148803.x 1 The preparation technology of ", this document discloses the method for adopting macroporous resin method+silica gel column chromatography+silica gel column chromatography to obtain ginsenoside Rb 1 , the same method is only applicable to laboratory research, and is difficult to realize industrial production; another major problem of this document is to obtain ginsenoside Rb 1 There is no purity, and there is no method for content determination; the patent document "A Method for Extracting and Separating Ginsenoside Monomers from Ginseng Leaves" with application number "200610093610.6" discloses the method of using macroporous resin method + silica gel column chromatography. Method for separating and obtaining different ginsenoside monomers, including ginsenoside Rb 1 ; Its main problem still does not have the requirement of purity, does not have assay method, and this technological method has still adopted column chromatography this complex, cost height, the method that can't industrialized production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] The total saponins of Panax notoginseng obtained by extracting Panax notoginseng were dissolved in water, filtered, and the filtrate was passed through a HPD-100 macroporous adsorption resin column, washed with water, eluted with 40% ethanol, and the eluent was discarded, then eluted with 50% ethanol, and collected 50% eluent, dried, the dried matter was dissolved in ethanol, filtered, added acetone to the filtrate, left to precipitate, filtered and dried to obtain ginsenoside Rb 1 .

[0079] The total saponins of Panax notoginseng are purchased commercially;

[0080] The above ginsenosides as Rb 1 Pharmaceutical raw materials can be prepared into pharmaceutically acceptable pharmaceutical preparations.

Embodiment 2

[0082] Take the total ginseng saponins extracted from ginseng, dissolve them in water, filter, and pass the filtrate through a HPD-100A macroporous adsorption resin column, wash with water, elute with 40% ethanol, discard the eluate, elute with 50% ethanol, and collect 50% The eluate was dried, and the dried product was dissolved in 95% ethanol, filtered, and the filtrate was added with organic solvent ethyl acetate, left to precipitate, filtered, and dried to obtain ginsenoside Rb 1 .

[0083] The extraction method of total ginseng saponins is prepared according to existing patent documents or scientific documents.

[0084] The above ginsenosides as Rb 1 Pharmaceutical raw materials can be prepared into pharmaceutically acceptable pharmaceutical preparations.

Embodiment 3

[0086] Extract the total saponins obtained from American ginseng, dissolve them in water, filter, and pass the filtrate through a HPD-300 macroporous adsorption resin column, wash with water, elute with 40% ethanol, discard the eluate, elute with 50% ethanol, and collect 50% eluate liquid, dried, and the dried matter was dissolved in n-butanol, filtered, and the filtrate was added with organic solvents acetone and ethyl acetate, left to precipitate, filtered, and dried to obtain ginsenoside Rb 1 .

[0087] The method for extracting total saponins of American ginseng is prepared according to existing patent documents or scientific documents.

[0088] The above ginsenosides as Rb 1 Pharmaceutical raw materials can be prepared into pharmaceutically acceptable pharmaceutical preparations.

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PUM

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Abstract

The invention discloses a new preparation method for ginsenoside Rb1, which is characterized in that TSSP (Total saponins of Panax notoginseng) is obtained by taking different drug ingredients; the ginsenoside Rb1, the purity of which is more than or equal to 90%, is obtained by a macroporous absorbent resin method and an organic solvent recrystal method. The method is suitable for commercial process, can obtain feather weight ginsenoside Rb1, and has extraction efficiency. Pharmacology experiment proves that the ginsenoside Rb1 obtained by the method has good pharmacological action.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a kind of ginsenoside Rb 1 Active ingredient preparation method. Background technique [0002] Ginsenoside Rb 1 It is mainly derived from the roots, stems, leaves, and flower buds of plants such as Araliaceae Panax ginseng, Panax notoginseng, American ginseng, Vietnamese ginseng, and bamboo ginseng, Cucurbitaceae Gynostemma genus Gynostemma, Gynostemma japonica and other plants; ginsenoside Rb 1 In addition to having good pharmacological activity in cardiovascular and cerebrovascular, further studies in recent years have shown that: ginsenoside Rb 1 It can activate RNA polymerase, promote the synthesis of RNA and DNA protein in nerve cells, repair damaged nerve cells, regenerate nerve cells, have a protective effect on cerebral cortex cells, improve thinking ability and memory, and prompt ginsenoside Rb 1 It has the value of developing intelligence-stimula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00A61K31/704A61P9/00A61P35/00A61P25/28
Inventor 顾群李志刚郭小鹏米长江阮爱华刘严渠守峰金治刚栗艳彬
Owner PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING