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Method for manufacturing liquid xylanase through fermentation

A technology of xylanase and production method, applied in microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of inability to hydrolyze xylanase, environmental pollution, large resource consumption, etc. The effect of using level, convenient operation and implementation, and simple production process

Inactive Publication Date: 2009-06-03
HENAN TIANGUAN GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, international attention has been attached to the research on this enzyme. However, the xylanase produced by the prior art mainly produces xylooligosaccharides when decomposing hemicellulose, and the content of xylose is relatively low, so it is not suitable for The use of cellulosic raw materials for alcohol production is mainly due to the lack of xylosidase in the cultured xylanase enzyme components, which can only convert hemicellulose into xylooligosaccharides, and cannot further hydrolyze xylooligosaccharides into available yeast The monosaccharides used have seriously hindered its industrial application in biomass fuel ethanol. How to improve xylanase activity and xylosidase activity in enzyme components, while high-enzyme activity and low-cost xylan Enzyme production techniques are currently unresolved
Therefore, the current industrial production of alcohol still uses grain as raw material, which consumes a lot of resources and has high production costs, which is not conducive to the popularization and use of fuel alcohol.
On the other hand, a large amount of hemicellulose-containing plant straws are usually discarded or burned, resulting in great waste and varying degrees of pollution to the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take the 50L fermenter as an example below to describe the present invention in detail:

[0015] First, select the Aspergillus niger mutant strain from the xylanase production strains as the production strain and store it on the PDA slant lawn for future use; Cultivate under the conditions for 2 days, then add the culture medium to the seed tank, then insert the culture bottle bacteria into the seed tank according to the weight percentage of 2% of the culture medium, and cultivate under the conditions of 28 temperature and ventilation ratio of 0.2 ~ 0.6v / v For 40 hours, wherein the culture medium was a bran-water mixture with a concentration of 2% by weight to obtain the production strain; 10% by weight of the production strain, 8% crushed corn cobs with a particle size below 0.5mm, and 6% corn slurry, 1% nutrient salt, and 75% water to form a fermented slurry, and put the fermented slurry into 5m 3 In the fermenter, carry out ventilation and stirring fermentation acco...

Embodiment 2

[0017] 5m below 3 Fermentation tank is taken as an example to illustrate the present invention in detail:

[0018] First, select the Aspergillus niger mutant strain from the xylanase production strains as the production strain and put it on the PDA slant lawn for future use; then pick the slant bacteria with a strong lawn and put it into a culture bottle, and put it in a culture bottle at a temperature of 30 ° C. Cultivate under the conditions for 35 hours, then add the culture medium to the seed tank, and then insert the bacteria in the culture bottle into the seed tank according to the weight percentage of 6% of the culture medium. Cultivate for 25 hours, wherein the culture medium is bran-water mixed solution of 4% concentration by weight to obtain production strains; 2% production strains by weight percentage, 2% crushed corn cobs with particle size below 0.5mm, 0.5% Mix corn steep liquor, 0.5% nutrient salt, and 95% water to form a fermented slurry, and put the fermented...

Embodiment 3

[0020] 50m below 3 Fermentation tank is taken as an example to illustrate the present invention in detail:

[0021] First, select the Aspergillus niger mutant strain from the xylanase production strains as the production strain and store it on the PDA slant lawn for later use; Cultivate under the conditions for 1 day, then add the culture medium to the seed tank, and then insert the strains of the culture bottle into the seed tank according to the weight percentage of 10% of the culture medium, under the conditions of 34°C temperature and ventilation ratio of 0.2-0.6v / v Cultivate for 12 hours, wherein the culture medium is bran-water mixed solution of 5% concentration by weight to obtain the production strain; 6% by weight of the production strain, 6% crushed corncobs with a particle size below 0.5mm, 3.3% Corn steep liquor, 0.7% nutrient salt, and 84% water are mixed into a fermented slurry, and the fermented slurry is put into a 50L fermenter for ventilating and stirring fe...

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PUM

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Abstract

The invention relates to a method for manufacturing liquid xylanase through fermentation. The method adopts aspergillus niger mutagenic fungi as the manufacture bacterial strain which is subjected to liquid deep level ventilation fermentation on a low sugar culture medium to manufacture xylanase preparations. The method can enable enzymic synthesis to grow rapidly without interruption. Xylanase manufactured by using the method is nontoxic and harmless, and can be applied to the fields of light industry paper making, food, energy, fodder, environment protection and the like, in particular to the mass production of plant fiber ethanol, thereby pioneering in the field of using agricultural waste to generate energy. Moreover, the method has the advantages of high ratio of enzyme activity to cost, straightforward technology, short fermentation period, and stable product quality, and is nontoxic and causes no pollution. Meanwhile, solid residues separated by fermentation from enzyme preparations contain a certain amount of nutrient substances such as xylanase, mycoprotein and the like, and therefore can achieve remarkable benefits when being used as feed additives.

Description

technical field [0001] The invention belongs to the technical field of preparation of enzyme bacteria, and relates to a fermentation production method of liquid xylanase. Background technique [0002] The research and development of biomass ethanol is the focus of new resource strategies of various countries. All countries in the world, especially developed countries such as the United States, Canada, Germany, Sweden, and Japan, are devoting themselves to the development of clean and efficient biomass energy utilization technologies, but currently there is no enterprise in the world that uses biomass raw materials to produce fuel ethanol on an industrial scale . The main obstacles are: the high cost of enzymes, and the low conversion efficiency of cellulose and hemicellulose to yeast-usable monosaccharides; [0003] Hemicellulose is the second most abundant polysaccharide in plant cell walls, accounting for 30-40% of the dry weight of biomass raw materials. Xylan is a pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42A23K1/16C12R1/685
Inventor 王林风杨付伟闫振丽吕世锋任建伟
Owner HENAN TIANGUAN GRP