Method for preparing hydrogen by fermenting biologically pretreated straw
A biological pretreatment and straw technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of fermentation hydrogen production difficulties, achieve the effect of simple and efficient process, solve the shortage of raw materials, and low cost
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Embodiment 1
[0023] Using biologically pretreated sorghum straw and mesophilic hydrogen-producing sludge to produce hydrogen by fermentation, the steps are as follows:
[0024] (1) Biological pretreatment
[0025] First, in a solid-state fermentation reactor, a nutrient salt solution (1.6% ammonium sulfate, 0.6% magnesium sulfate heptahydrate, 0.3% potassium dihydrogen phosphate) was added to the sorghum straw at a solid-to-liquid ratio of 1 / 2.5, and stirred evenly.
[0026] Then, Trichoderma reesei Rut C30 was slanted with conventional PDA medium, and the seed medium was inoculated with Trichoderma reesei Rut C30, and cultured at 28°C for 4 days to obtain Trichoderma reesei Rut C30 seed liquid. The seed medium was 5% spent grains, 0.5% glucose, 0.8% ammonium sulfate, pH 6.2.
[0027] Finally, the above-mentioned Trichoderma reesei seed liquid was inoculated into a solid-state fermentation reactor with an inoculation amount of 10%, the fermentation temperature was 25° C., and the fermenta...
Embodiment 2
[0034] Using biologically pretreated corn stover and high-temperature hydrogen-producing sludge to ferment hydrogen to produce hydrogen, the steps are as follows:
[0035] (1) Biological pretreatment
[0036] First, in a solid-state fermentation reactor, a nutrient salt solution (1.6% ammonium sulfate, 0.6% magnesium sulfate heptahydrate, 0.3% potassium dihydrogen phosphate) was added to the corn stover at a solid-to-liquid ratio of 1 / 2.5, and stirred evenly.
[0037] Then, Trichoderma reesei Rut C30 was slanted with conventional PDA medium, and the seed medium was inoculated with Trichoderma reesei Rut C30, and cultured at 28°C for 4 days to obtain Trichoderma reesei Rut C30 seed liquid. The seed medium was 5% spent grains, 0.5% glucose, 0.8% ammonium sulfate, pH 6.2.
[0038] Finally, the above-mentioned Trichoderma reesei seed liquid was inoculated into a solid-state fermentation reactor with an inoculation amount of 10%, the fermentation temperature was 30° C., and the ferm...
Embodiment 3
[0045] Using biologically pretreated wheat straw and high-temperature hydrogen-producing sludge to ferment hydrogen to produce hydrogen, the steps are as follows:
[0046] (1) Biological pretreatment
[0047] First, in the solid-state fermentation reactor, a nutrient salt solution (1.6% ammonium sulfate, 0.6% magnesium sulfate heptahydrate, 0.3% potassium dihydrogen phosphate) was added to the wheat straw according to a solid-liquid ratio of 1 / 2.5, and stirred evenly.
[0048] Then, Trichoderma reesei Rut C30 was slanted with conventional PDA medium, and the seed medium was inoculated with Trichoderma reesei Rut C30, and cultured at 28°C for 4 days to obtain Trichoderma reesei RutC30. The seed medium was 5% spent grains, 0.5% glucose, 0.8% ammonium sulfate, pH 6.2.
[0049] Finally, the above-mentioned Trichoderma reesei seed liquid was inoculated into a solid-state fermentation reactor with an inoculation amount of 10%, the fermentation temperature was 32° C., and the fermen...
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