Streptomycete and use thereof

Abstract

The invention discloses a streptomycete and the application thereof. The strain of the streptomycete is collected in China Center for Type Culture Collection (CCTCC); the collection number of the strain is CCTCC No. M 207201; and the collection date of the strain is Dec. 14, 2007. The invention further discloses a method for using the strain to ferment and prepare Tacrolimus. The fermentation process provided by the invention is tested in shake flasks and 10 tons of fermentation cylinders. The fermentation process has the advantages of stable production capacity, high fermentation unit, less byproducts of fermentation, and suitability for industrial production. In addition, the fermentation process reduces the difficulty of post-extraction.

Description

technical field [0001] The present invention relates to a novel microorganism and its use and application, in particular to a streptomyces and its application in the preparation of tacrolimus. Background technique [0002] Immunosuppressants are used to prevent rejection in organ transplantation, that is, to interfere with the receptor's recognition of foreign antigens and the removal of non-self cells. Since the first successful kidney transplant between identical twins in 1954, there have been many major breakthroughs in medical science research on organ transplantation over the past 40 years. The clinical use of cyclosporine A (cyclosporine A) in 1978 ushered in a new era for organ transplantation. The 2-year survival rate of most patients with kidney transplantation has exceeded 80%, and the survival rate of patients has also improved. [0003] Tacrolimus (also known as FK506) is a biologically active substance produced by Streptomyces with a high degree of immunosuppr...

AI Technical Summary

Problems solved by technology

General tacrolimus-producing bacteria have very low fermentation units and more fermentation by-products, resulting in more complicated post-extraction work

The patent USP4894366 published by Okuhara et al. mentioned the extraction and purification process of tacrolimus. The fermentation broth of Streptomyces tsukubaensis No.9993 strain was filtered to obtain the bacterial residue, which was extracted with acetone, and the extract was adsorbed and desorbed by HP20 resin. The desorption solution was concentrated, extracted with ethyl acetate, and the ethyl acetate phase was concentrated to obtain an oily crude product, which was then purified by several times of special silica gel chromatography, and finally crystallized and recrystallized in acetonitrile to obtain tacrolimus, The whole extraction and purification process is very complicated, especially after several times of chromatography with special silica gel, it is difficult to operate in production, the consumption of solvent and energy is high, and the yield of finished product is low