Glucose oxidase single liquid detection reagent

Abstract

The invention discloses a reagent for detecting single liquid of glucose oxidase, which is in a state of single liquid. The reagent comprises the following components: 1 to 50KU/L of glucose oxidase, 0.5 to 50 KU/L of peroxidase, 0.1 to 20mmol/L of 4-amino-antipyrine, 1 to 200g/L of sodium sulfate, 5 to 500mmol/L of 3-Morpholinopropanesulfonic acid, 0.05 to 10g/L of highly sensitive tertiary color source, 0.05 to 20g/L of antiseptic agent, and buffer solution of which the pH value is between 5.0 and 8.0. The reagent has the advantages of existence in a state of single liquid, excellent stability and sensitivity, wide detection wavelength range and the like.

Description

technical field [0001] The invention relates to an analysis reagent in the field of medical testing, in particular to a glucose oxidase single liquid assay reagent for measuring glucose content. Background technique [0002] Numerous research results show that the global incidence of diabetes is increasing rapidly, and diabetes has become the third chronic disease that seriously threatens human health after tumors and cardiovascular diseases. At present, there are more than 120 million people with diabetes in the world, and the number of patients in my country ranks second in the world. Since diabetes is a lifelong disease, for patients with diagnosed diabetes, in order to control and delay the development of the disease and reduce the occurrence of complications, blood sugar must be measured regularly and irregularly. As one of the blood routine items in the hospital, how to quickly and accurately detect blood Glucose content has positive significance. [0003] The enzyma...

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Problems solved by technology

Among them, the reagents of the hexokinase method and the glucose dehydrogenase method are expensive (2-3 times that of the oxidase method) and cannot be detected in the visible light range; while the enzyme electrode method is not popular and cannot be combined for detection on clinical biochemical analyzers; Although the blood glucose meter is convenient in a small amount, it can only be used for qualitative detection; the glucose oxidase method is economical and accurate, and is currently the most widely used method for measuring blood sugar, but the common glucose oxidase method with phenol as the chromogen mixed reagent is not suitable Stable, the enzyme-phenol mixed reagent can only be stored for 1 month in the refrigerator (refer to "National Clinical Inspection Operation Procedures (3rd Edition)", P360-361 compiled by the Medical Administration Department of the Ministry of Health, published by Southeast University Press in November 2006), and applicable The wavelength range is narrow. Therefore, the glucose oxidase method reagents currently on the market often use freeze-dried reagents or liquid double reagents to prolong the storage period. There are many reagent compartments in the instrument, which are easy to be misplaced. In addition, the loss of the instrument causes the ratio of the two reagents to be unbalanced, resulting in a large waste of reagents. The reconstitution of the freeze-dried reagents brings additional workload to hospital users, and reconstitution is more likely to cause secondary pollution. Affect the quality and efficiency of the inspection. In addition, the glucose oxidase method reagents containing phenol on the market will cause great harm and pollution to operators, equipment and the environment.

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