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Bacteria cellulose compound film, preparation and uses thereof

A technology of bacterial cellulose and composite membranes, applied in biochemical equipment and methods, methods based on microorganisms, medical science, etc., can solve the problems of spreading diseases, expensive, storage, transportation needs, etc., and achieve simple preparation process and low cost low, conversion-increasing effect

Inactive Publication Date: 2009-08-19
SHANGHAI INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Low-temperature freezing (below -80°C) allograft skin is a widely used clinical product, but it has the following disadvantages: first, liquid nitrogen tanks are required for storage and transportation, which is expensive and inconvenient to use, especially in emergency situations; After terminal sterilization, there is a risk of spreading diseases; third, it is a living tissue with strong immunogenicity, which dissolves and rejects after transplantation, and stays on the wound for no more than 2 weeks
[0004] Bacterial cellulose is a nano-scale fiber material with high water content. After modification, the water content is higher and the yield is also increased. Therefore, the application of the modified bacterial cellulose composite film as a wound dressing has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Strain selection: Gluconacetobacter xylinum CGMCC No.1.1812 was selected. .

[0033] (2) Preparation of seeds and liquid culture medium: in terms of weight percentage, containing 2% fructose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4 , 0.2% citric acid, 2% calcium carbonate seed medium or liquid medium with 0.05% sodium alginate added, natural pH value, sterilized at 121°C for 20min, and the seeds can be obtained after cooling to 30°C Medium for culture and liquid fermentation culture.

[0034] (3) Seed cell culture: Take the slanted seeds (Gluconacetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.1812) that have been activated in the 1-2 rings and insert them into the medium for seed culture, shake and cultivate at 30°C for 24 hours, and the shaker speed is 160r / min, get the seed solution;

[0035] (4) Bacterial cellulose produced by static liquid fermentation: inoculate the seed liquid into the culture medium of liquid fermentation culture with ...

Embodiment 2

[0039] (1) Strain selection: Gluconacetobacter xylinum CGMCC No.1.2378 was selected.

[0040] (2) Preparation of medium for seed culture and liquid culture: calculate by weight percentage, get 2% glucose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4 , 0.2% citric acid, 2% calcium carbonate, 0.05% sodium alginate, adjust the pH to 6.0 with citric acid or acetic acid, sterilize at 121°C for 20 minutes, and cool to 30°C to obtain the medium for seed culture and liquid fermentation culture .

[0041] (3) Seed cell culture: Take a ring of activated slant seeds (Gluconacetobacter xylinum) CGMCC No.1.2378, insert it into the medium for seed culture, and culture it with shaking at 30°C for 24 hours, and the speed of the shaker is 160r / min , as a seed solution.

[0042] (4) Static liquid fermentation produces bacterial cellulose: inoculate in the substratum of liquid fermentation culture (500mL triangular flask is filled with 200mL liquid medium) with 6% inoculum size, need ful...

Embodiment 3

[0046] (1) Strain selection: select Gluconacetobacter xylinum CGMCC No.1.1812;

[0047] (2) Preparation of medium for seed culture and liquid culture: calculate by weight percentage, get 2% glucose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4 , 0.2% citric acid, 2% calcium carbonate, 0.04% sodium alginate, adjust the pH to 6.0 with citric acid or acetic acid. Sterilize at 121°C for 20 minutes, and cool to 30°C to obtain the medium for seed culture and liquid fermentation culture .

[0048] (3) Seed cell culture: Take a ring of activated slant seeds (Gluconacetobacter xylinum) CGMCC No.1.1812, insert it into the medium for seed culture, and cultivate it with shaking at 32°C for 24 hours, and the shaker speed is 160r / min , as a seed solution.

[0049] (4) Bacterial cellulose produced by static liquid fermentation: inoculate the seed liquid into the culture medium of liquid fermentation with 7% inoculum amount (distributed in the fermentation shallow dish, after the ferm...

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PUM

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Abstract

The invention relates to a bacterial cellulose composite membrane, a preparation method and application thereof; the preparation steps include the selection of a strain, the preparation of a seed and liquid medium, the culture of a seed cell, the production of a bacterial cellulose composite membrane by liquid fermentation and separation as well as purification and the like, finally the bacterial cellulose composite membrane is prepared. The bacterial cellulose composite membrane which is obtained by the invention has simple preparation process and low cost, significantly increases the conversion rate of raw materials and the output and water content of bacterial cellulose, can cause the dry weight of bacterial cellulose to be improved from the traditional 8-10g / L to 14-16g / L, and leads the water content to be raised from 98 to 99%. The bacterial cellulose composite membrane which is obtained by the invention can be used as a wound dressing.

Description

Technical field: [0001] The invention relates to a wound dressing material, a production method and application thereof, in particular to a bacterial cellulose composite film and a preparation method and application thereof. Background technique: [0002] Bacterial cellulose was discovered by Brown in 1886. Acetobacter xylinum formed a layer of white fibrous substance on the surface of the medium during static culture. It was determined by chemical and physical analysis that this substance has the structure and structure of cellulose. Chemical properties, named bacterial cellulose (bacterial cellulose BC) because it is synthesized by bacteria. The diameter of bacterial cellulose is only 1 / 10 of that of artificial synthetic fibers, ranging from 10nm to 100nm. It is a new type of nano-scale biological material. It has many unique properties, such as high crystallinity and high chemical purity, high tensile strength and elastic modulus, strong water binding, excellent shape re...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08J5/18C08L1/02C08L5/04A61L15/28C12R1/01
Inventor 马霞张华
Owner SHANGHAI INST OF TECH
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