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Preparation method of injectable human hair keratin soft tissue filling material

A filling material and keratin technology, which is used in medical science, surgery, etc., to achieve the effect of convenient acquisition, long-term repair, and repeated processing of materials

Active Publication Date: 2012-02-22
泛亚润康(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Each material has its own advantages and disadvantages, such as: hyaluronic acid gel has strong heterogeneity and is easy to be absorbed; purified bovine collagen, including Zyderm I (containing collagen 35mg / mL), ZydermH ( Containing collagen 65mg / mL) and Zyplast (containing glutaraldehyde-crosslinked collagen 35mg / mL) in the tissue for only 6-9 months, and will cause local allergic reactions in the injection area of ​​1%-3% of healthy patients , manifested as temporary erythema, itching symptoms, induration and swelling; polymethyl methacrylate (PMMA) particles can easily lead to complications such as allergic reactions, telangiectasia, and granuloma formation, and PMMA is an inert material that cannot be degraded. Difficult to remove after insertion
The complications of silica gel injection are mostly granuloma formation, hardening, skin thinning, ulceration, etc. in the surrounding tissue; polyacrylamide hydrogel is easy to cause bleeding, hematoma, infection, local edema, induration, and asymmetry; Although polytetrafluoroethylene (ePTFE) is stable in nature and has good tissue compatibility, it is very easy to absorb or inhale impurities and store them in its internal micropores, which makes it lose its good tissue compatibility and is harmful to surgical operations. The requirements are higher and the price is more expensive; the long-term efficacy of autologous fat particles and autologous fibroblast plasma as soft tissue filling materials is not clear

Method used

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  • Preparation method of injectable human hair keratin soft tissue filling material
  • Preparation method of injectable human hair keratin soft tissue filling material
  • Preparation method of injectable human hair keratin soft tissue filling material

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preparation example Construction

[0021] A preparation method of an injectable human hair keratin soft tissue filling material, the steps are as follows:

[0022] (1) Cleaning to remove impurities: at room temperature, take the middle section of the human hair sample, wash it repeatedly with distilled water, and dry it;

[0023] (2) Degreasing: at room temperature, immerse the cleaned human hair in ethanol degreasing solution for 1.5-2 hours, wash with distilled water, and dry;

[0024] (3) Chlorination: NaClO and dilute H 2 SO 4 , water-bath ratio 1:30, react at room temperature for 20 minutes, rinse with running water;

[0025] (4) Bleaching: adopt room temperature bleaching method, clean human hair hair 20~30 mass parts, mass volume concentration is H 2 o 2 30 parts by mass, 8 parts by mass of sodium pyrophosphate, 60 parts by mass of ammonia water, 3 parts by mass of potassium persulfate, and react at room temperature for 4 hours;

[0026] (5) Cleaning and drying: After the human hair raw material tu...

Embodiment 1

[0030] 1.1 Experimental materials, reagents and instruments

[0031] CO 2 Cell culture incubator (Heal Force BB16UV HongKong), phase contrast microscope (NikonEclipse TS100 Japan), 96-well plate, DMEM medium (Gibco, USA), fetal bovine serum (Hangzhou Sijiqing), PBS buffer, MTT (bromide-3 -(4-5-Dimethylthiazolyl-2)2,5 diphenyltetrazolium, Sigma company USA) concentration is 5mg / ml (0.5%), filter sterilization, DMSO (Sigma company, USA), physiological Saline, autoclaved, 30% hydrogen peroxide, concentrated ammonia, potassium persulfate.

[0032] 1.2 Human hair processing

[0033] Get 20g middle section human hair after degreasing washing drying, chlorination (0.32%NaClO, 0.5%H 2 SO 4 , water bath ratio 1:30, 20°C, 20 minutes), rinse with running water, soak in double distilled water for 3 times, bleach (30% H 2 o 2 , sodium pyrophosphate 8g / l, potassium persulfate 3g / l, ammonia water 14g / l, water-to-bath ratio 1:100, water bath at 40°C for 3.5h), dry at 30°C, weigh and tak...

Embodiment 2

[0061] 1 Animal model preparation

[0062] Mark 5 injection sites along the left and right sides of the central axis in the hair collection area on the back of each rabbit, and inject the prepared four kinds of autologous rabbit hair keratin powders A, B, C, and D subcutaneously with a No. 8 needle after skin preparation About 2.5ml of the homogenized material was used to form a subcutaneous bulge with a diameter of about 2cm, and another part was injected with normal saline as a control. The in vivo absorbability of the material was investigated using the soft tissue filling material injection animal model designed by Marler et al. Use vernier calipers to measure the Pichu parameters at month i after injection: Pichu length a i , Picchu width b i , Pichu height c i , to calculate the picchu volume V i (Formula 1), and Pichu volume retention δ i (Formula 2). The higher the δ, the lower the absorptivity of the material. Calculate the four δ values ​​of A, B, C, and D, an...

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Abstract

The invention relates to the technical field of medical plastic surgery. According to the advantages that human hair has high resistance to physical and chemical factors, stable properties, acid and alkali resistance, is not easy to be absorbed and degraded by tissues, is easy to obtain, can be repeatedly obtained, and is easy to process, it overcomes the existing There are deficiencies of soft tissue filler materials. Using the middle section of solid autologous human hair as raw material, after cleaning, bleaching, grinding, freeze-drying, packaging, and cobalt 60 irradiation treatment, the bleached human hair is made into powder homogenate and liquid human hair keratin particles of different thicknesses , to provide a human hair keratin soft tissue filling material that is derived from autologous body, is easy to obtain, is safe and non-toxic, has good tissue compatibility, is easy to be tolerated by the human body, and can stimulate collagen proliferation and is suitable for injection. Realize the long-term repair of skin and soft tissue, and meet the needs of soft tissue filling materials in the field of plastic surgery.

Description

technical field [0001] The invention relates to a material for medical plastic surgery, in particular to a preparation method of an injectable human hair keratin soft tissue filling material derived from autologous body and suitable for injection. Background technique [0002] Soft tissue filling materials have extensive and important applications in the field of plastic surgery. In the treatment of tissue defect repair and improvement of facial and body contours, ideal results can be achieved through soft tissue filling materials; compared with surgical methods, the method of injecting soft tissue filling materials is simple and practical, easy to master, and easy to control the shape. [0003] However, this method has high requirements for filling materials. The ideal filling material should have the following characteristics: 1) It is preferably autologous material, and there is no immune rejection problem; 2) It can be stored for a relatively long time, but not necessar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04
Inventor 章庆国
Owner 泛亚润康(北京)生物科技有限公司
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