Phenyl propanoid derivative, preparation method thereof and application thereof to preparation of medicines resisting breast cancer
A derivative, phenylpropanoid technology, applied in the application field of phenylpropanoid derivatives and its preparation, preparation of anti-breast cancer drugs, achieving high purity, accurate results, and broad application prospects
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[0032] Example 1 Preparation of compounds represented by formula (I) and formula (II)
[0033] In this example, the extraction, extraction, and the combination of column chromatography and thin-layer chromatography commonly used by those skilled in the art to separate and obtain an active ingredient from a plant are used to obtain two new species from the stems and branches of the mangrove pomegranate. substances, and they were identified by spectral signal analysis. It was found that the structural formulas of these two substances are shown in formula (I) and formula (II), and these two compounds are a pair of epimers, belonging to phenylpropanoid compounds. , but with a new skeleton structure different from the existing phenylpropanoid compounds.
[0034] The preparation method of the present embodiment, its concrete steps are as follows:
[0035] (1) the stems and branches of the mangrove pomegranate are naturally air-dried and pulverized, take 4.9Kg of the pulverized mate...
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[0050] Example 2 The lethal activity experiment of Catunaregin and Epicatunaregin to saltwater shrimp
[0051] Take a 96-well cell culture plate, add 200 μl of artificial seawater solution containing 10-15 sea shrimp larvae to each well, and prepare a test culture plate.
[0052] In this example, a blank control group and a sample group were set: the blank control group was prepared by adding 5 μl of the solvent dimethyl sulfoxide (DMSO) to the above-mentioned test culture plate; the sample group was prepared by dissolving the Catunaregin prepared in Example 1 with the solvent DMSO. Catunaregin content of 500μg / mL, 50μg / mL and 5μg / mL three concentrations of sample solution, three groups of sample components, 1 group is to add 5μl concentration of 500μg / mL sample solution to the above test culture plate, 2 groups 5 μl of the sample solution with a concentration of 50 μg / mL was added to the above-mentioned test culture plate, and 5 μl of the sample solution with a concentration ...
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[0060] Example 3 Determination of antitumor activity of Catunaregin and Epicatunaregin by MTT method
[0061] In this example, tumor cell-human breast cancer F10 (commercially available) was selected as the research object, and the MTT colorimetric method routinely used by those skilled in the art to detect cell survival and growth was used to conduct tumor cell growth inhibition experiments on Catunaregin and Epicatunaregin.
[0062] Sample group: After dissolving the Catunaregin prepared in Example 1 in DMSO, five sample groups with Catunaregin content of 0.01, 0.1, 1, 10 and 100 μg / mL were prepared.
[0063] Positive control group: 5-fluorouracil.
[0064] Negative control group: complete cell culture medium (commercially available) in 0.5 vol% DMSO.
[0065] F10 cells in logarithmic growth phase were collected and seeded in 96-well culture plates, and the number of cells per well was 1.0×10 5 / 100μl in 5 vol% CO 2 After 48 hours, 10 μl of MTT was added to each well, and...
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