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Online production method of rhamnolipid biosurfactant in cellulose hydrolyzation

A cellulase and biological surface technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the technical report on the online production and addition method of biosurfactants, and increase the production cost of rhamnolipids. and other problems to achieve the effect of reducing production cost, simplifying production process and improving hydrolysis efficiency

Inactive Publication Date: 2009-09-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method of separating and purifying before adding will greatly increase the production cost of rhamnolipids
[0004] In the cellulase hydrolysis system, there is no relevant technical report on the online production and addition of biosurfactants

Method used

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  • Online production method of rhamnolipid biosurfactant in cellulose hydrolyzation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Rinse the harvested straw with tap water 4-5 times to remove impurities and knots, and cut it into small sections of 1-2cm. The cut straw was dried in an oven at 70 °C to constant weight and stored at room temperature for subsequent use. Before enzymatic hydrolysis, the straw that cuts is carried out pretreatment with alkali, and concrete method is: the straw that cuts 10g is placed in the Erlenmeyer flask that the sodium hydroxide of 40ml 2% is housed, keeps solid-liquid mass ratio as 1:4, 85°C water bath for 1h. Wash with distilled water until neutral, and dry in an oven at 70°C until constant weight.

[0026] The pretreated rice straw was hydrolyzed with Trichoderma reesei ZM4-F3 in a shaker at 30° C. and 200 rpm for sugar production.

[0027] Then the Pseudomonas aeruginosa BSZ-07 cultivated to the logarithmic growth phase is inoculated into the rice straw hydrolysis system with 2% inoculum, that is: Pseudomonas aeruginosa BSZ-07 is dissolved in 50mL seed medium t...

Embodiment 2

[0034] Rinse the harvested straw with tap water 4-5 times to remove impurities and knots, and cut it into small sections of 1-2cm. The cut straw was dried in an oven at 70 °C to constant weight and stored at room temperature for subsequent use. Before enzymatic hydrolysis, the straw that cuts is carried out pretreatment with alkali, and concrete method is: the straw that cuts 10g is placed in the Erlenmeyer flask that the sodium hydroxide of 40ml 2% is housed, keeps solid-liquid mass ratio as 1:4, 85°C water bath for 1h. Wash with distilled water until neutral, and dry in an oven at 70°C until constant weight.

[0035] The pretreated rice straw was hydrolyzed with Trichoderma reesei ZM4-F3 in a shaker at 30° C. and 200 rpm for sugar production.

[0036] Then inoculate the Pseudomonas aeruginosa BSZ-07 cultivated to the logarithmic growth phase into the rice straw hydrolysis system with 4% inoculum: dissolve the Pseudomonas aeruginosa BSZ-07 in 50mL seed medium to prepare the...

Embodiment 3

[0043] Rinse the harvested straw with tap water 4-5 times to remove impurities and knots, and cut it into small sections of 1-2cm. The cut straw was dried in an oven at 70 °C to constant weight and stored at room temperature for subsequent use. Before enzymatic hydrolysis, the straw that cuts is carried out pretreatment with alkali, and concrete method is: the straw that cuts 10g is placed in the Erlenmeyer flask that the sodium hydroxide of 40ml 2% is housed, keeps solid-liquid mass ratio as 1:4, 85°C water bath for 1h. Wash with distilled water until neutral, and dry in an oven at 70°C until constant weight.

[0044] The pretreated rice straw was hydrolyzed with Trichoderma reesei ZM4-F3 in a shaker at 30° C. and 200 rpm for sugar production.

[0045] Then the Pseudomonas aeruginosa BSZ-07 cultivated to the logarithmic growth phase was inoculated into the rice straw hydrolysis system with 6% inoculum: Pseudomonas aeruginosa BSZ-07 was dissolved in 50mL seed medium to prepa...

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Abstract

The invention relates to an online production method of rhamnolipid biosurfactant in cellulose hydrolyzation, belonging to the field of biotechnology. Firstly, reaped straws are cleaned, cut and dried to constant weight, and then the cut straws are treated by alkali before cellulose hydrolyzation; secondly, the pretreated straws are cultured with Trichoderma reesei ZM4-F3 in a shaking table for hydrolyzation and sugar production for producing reducing sugar; thirdly, pseudomonas aeruginosa BSZ-07 during logarithmic phase obtained by culture is inoculated into a hydrolyzation system, thus realizing the online production of rhamnolipid biosurfactant and accelerating the hydrolyzation of cellulose. Owing to the addition method of the online production, the complex process of rhamnolipid purification can be saved, thus effectively reducing the production cost of rhamnolipid biosurfactant and being applicable for industrial production. Besides, the addition of rhamnolipid biosurfactant can improve the hydrolyzation efficiency of cellulose effectively.

Description

technical field [0001] The invention relates to a production method of a surfactant in the field of biotechnology, in particular to an on-line production method of a rhamnolipid biosurfactant in cellulase hydrolysis. Background technique [0002] The amphiphilic compound secreted by microorganisms during the metabolic process has surface activity and interface activity, and integrates hydrophilic group and hydrophobic group structure in the same molecule, which is called biosurfactant. Biosurfactants include many different classes, such as glycolipids, lipopeptides, polysaccharide-lipid complexes, phospholipids, fatty acids, and neutral lipids. Biosurfactants can promote the dispersion and absorption of insoluble organic matter in soil, and can increase the contact surface between hydrophobic organic compounds and liquid phase, thereby enhancing microbial activity, improving cellulose degradation rate and composting efficiency. In addition, biosurfactants can also play a gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/14C12P19/02C12R1/885C12R1/385
Inventor 蔡伟民张秋卓
Owner SHANGHAI JIAO TONG UNIV
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