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Method for preparing glycosylated ordered pore membrane by controllable glycerol polymerization

A technology of graft polymerization and porous membranes, which is applied in the field of preparation of ordered porous membranes, can solve the problem of not being able to control the density of glycosyl groups on the surface of ordered porous membranes according to needs, and achieve good application prospects, controllable glycosyl distribution and density , the effect of mild reaction conditions

Inactive Publication Date: 2009-10-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the glycosylated ordered porous membrane has an ordered surface with highly enriched glycosyl groups in a specific region, it can give full play to the clustering effect of glycosyl-recognized proteins and significantly improve the detection sensitivity of proteins, but it cannot control the ordering as required. Surface Glycan Density of Porous Membranes

Method used

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  • Method for preparing glycosylated ordered pore membrane by controllable glycerol polymerization
  • Method for preparing glycosylated ordered pore membrane by controllable glycerol polymerization
  • Method for preparing glycosylated ordered pore membrane by controllable glycerol polymerization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The molecular weight of the block copolymer is 10,000, and the styrene / hydroxyethyl methacrylate block copolymer with a molar percentage of 0.5% of hydroxyethyl methacrylate is dissolved in carbon disulfide to form a concentration of 15% by weight. % polymer solution.

[0032] Take 0.1mL of the above polymer solution and apply it to the clean glass surface, and form a film at a relative humidity of 50% at 5°C to obtain an area of ​​about 4cm 2 Ordered porous film; observed with a scanning electron microscope, the ordered porous film, the micropore diameter is 0.2 microns.

[0033] Put the above-mentioned ordered porous membrane, 5mL of n-heptane and 0.5mL of triethylamine in a flask, drop 0.3mL of bromoisobutyryl bromide at 0°C, react for 5 hours, take out the ordered porous membrane and wash it with methanol and water 3 times, and dried to constant weight to obtain an ordered porous membrane with immobilized bromine groups on the surface.

[0034] Then, the above-men...

Embodiment 2

[0036] The molecular weight of the block copolymer is 200,000, and the molar percentage of hydroxyethyl methacrylate is 10%. The styrene / hydroxyethyl methacrylate block copolymer is dissolved in carbon disulfide, and the concentration by weight is 5 % polymer solution.

[0037] Take 0.1mL of the above polymer solution and apply it to the clean glass surface, and form a film at a relative humidity of 90% and 50°C to obtain an area of ​​about 4cm 2 Ordered porous film; observed with a scanning electron microscope, the ordered porous film, the micropore diameter is 2.5 microns.

[0038] Put the above-mentioned ordered porous membrane, 50mL of n-heptane and 5mL of triethylamine in a flask, drop 3mL of bromoisobutyryl bromide into the flask at 0°C, react for 12 hours, take out the ordered porous membrane and wash it three times with methanol and water , and dried to constant weight, an ordered porous membrane with bromine groups immobilized on the surface was obtained.

[0039]Th...

Embodiment 3

[0041] The molecular weight of the block copolymer is 50,000, and the styrene / hydroxyethyl methacrylate block copolymer with a molar percentage of hydroxyethyl methacrylate of 10% is dissolved in carbon disulfide to form a concentration of 5% by weight. % polymer solution.

[0042] Take 0.1mL of the above polymer solution and apply it to the clean glass surface, and form a film at a relative humidity of 90% and 50°C to obtain an area of ​​about 4cm 2 Ordered porous film; observed with a scanning electron microscope, the ordered porous film, the micropore diameter is 2.5 microns.

[0043] Put the above-mentioned ordered porous membrane, 20 mL of n-heptane and 1.5 mL of triethylamine in a flask, drop 1 mL of bromoisobutyryl bromide into the flask at 0°C, react for 24 hours, take out the ordered porous membrane and wash it with methanol and water for 3 times, and dried to constant weight to obtain an ordered porous membrane with immobilized bromine groups on the surface.

[004...

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Abstract

The invention discloses a method for preparing glycosylated ordered pore membrane by controllable glycerol polymerization; a styrene block copolymer containing hydroxide radicals is used as a base material and a water-assisted method is adopted to prepare the ordered pore membrane; the glycerol polymerization of the surface atom transfer radical is initiated by the surface-initiated atom transfer radical polymerization under the existence of a catalyst to obtain the glycosylated ordered pore membrane. The method for preparing glycosylated ordered pore membrane by controllable glycerol polymerization is characterized by simple method, mild technical condition, low production cost as well as controllable glycosyl distribution and density of the prepared glycosylated ordered pore membrane, and can be applied to the fields of protein detection and cell culture and the like.

Description

technical field [0001] The invention relates to a method for preparing an ordered porous membrane, in particular to a method for preparing a glycosylated ordered porous membrane through controllable graft polymerization. Background technique [0002] In recent years, micron, submicron and nanoscale ordered porous materials have attracted extensive attention due to their application prospects in many fields such as catalyst loading, gas sensing devices, optoelectronic devices, cell culture substrates, separation or adsorption media, etc. At present, a series of methods for preparing ordered porous materials have been developed, such as lithography, etching, template method, self-assembly method and water-assisted method, among which the preparation of ordered porous membrane by water-assisted method has simple process and good experimental conditions It has the advantages of mildness and regular and controllable membrane structure. [0003] The Japanese patent publication No...

Claims

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Application Information

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IPC IPC(8): C08J9/40C08J9/28C08L53/00G01N33/68
Inventor 徐志康柯蓓蓓万灵书
Owner ZHEJIANG UNIV
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