Method for preparing biodegradable blood vessel external scaffold material used in tissue engineering

A technology of tissue engineering and scaffold materials, which is applied in the field of preparing biodegradable tissue engineering vascular outer layer scaffold materials, can solve the problems of poor mechanical properties and fast degradation rate of scaffold materials, and achieve excellent immunocompatibility and immune rejection The effect of low reaction and elimination of virus hazards

Inactive Publication Date: 2009-10-21
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although both collagen and chitosan have good biocompatibility, the scaffold materials constructed solely with collagen or chitosan have poor mechanical properties, and the degradation rate is too fast to meet the requirements of tissue engineering. Cross-linked and modified to have high mechanical properties and a degradation rate matched to tissue regeneration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Dissolve human-like collagen with distilled water into a 1.0% solution, and dissolve chitosan with a degree of deacetylation of 60% and a molecular weight of 400,000 daltons into a 1.0% solution with 0.5% acetic acid, and then press the two to 5 :1 (mass ratio) mix, add 0.4% of the total solution of glycerol, stir evenly, then add 40μL of 25% glutaraldehyde aqueous solution, gently stir for 30min, after filtering, vacuum degassing, pour into the tubular mold, 4 After being cross-linked at ℃ for 2 days, it was placed in a refrigerator at -80℃ for 5h to form, and vacuum dried at -10℃ to form an intravascular stent. After washing 5 times with distilled water, it was again freeze-dried and formed. 60 Standby after disinfection.

Embodiment 2

[0025] Dissolve human-like collagen with distilled water into a 2.0% solution, dissolve chitosan with a degree of deacetylation of 70% and a molecular weight of 450,000 daltons into a 1.5% solution with 0.5% acetic acid, and then press the two :1 (mass ratio) mix, add 0.4% of the total solution of glycerol, stir evenly, then add 20μL of 50% glutaraldehyde aqueous solution, gently stir for 30min, after filtering, vacuum degassing, pour into the tubular mold, 4 After being cross-linked at ℃ for 2 days, it was placed in a refrigerator at -80℃ for 5h to form, and vacuum dried at -10℃ to form an intravascular stent. After washing 5 times with distilled water, it was again freeze-dried and formed. 60 Standby after disinfection.

Embodiment 3

[0027] Dissolve human-like collagen with distilled water into a 1.0% solution, dissolve chitosan with a degree of deacetylation of 80% and a molecular weight of 500,000 Daltons into a 0.5% solution with 0.5% acetic acid, and then press the two :1 (mass ratio) mix, add 0.4% of the total solution of 1.3-butanediol, stir evenly, then add 40μL of 25% glutaraldehyde aqueous solution, gently stir for 30min, after filtering, vacuum degassing, inject into the tubular mold After cross-linking at 4°C for 3 days, it was placed in a -80°C refrigerator for 5h to form, and vacuum dried at -10°C to form an intravascular stent. After washing with distilled water for 5 times, it was again freeze-dried and molded. 60 Standby after disinfection.

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Abstract

The invention discloses a method for preparing a biodegradable blood vessel external scaffold material used in tissue engineering, comprising the steps as follows: using distilled water to dissolve human-like collagen to be a 1%-3% solution; using diluted acid to dissolve chitosan to be a 0.5%-1.5% solution; mixing the two solutions evenly, adding aqueous glutaric dialdehyde solution with the concentration of 20-50% according to mass percentage, mixing and stirring, and filtering followed by vacuum de-foaming; pouring the mixed solution into a tube-shaped die, cross-bonding at 4 DEG C for 1-3 days, placing the same into a refrigerator with the temperature being negative 80 DEG C for refrigerating, and then drying to obtain the blood vessel external scaffold material. Compared with the prior art, the prepared scaffold material has excellent mechanicalness, bio-compatibility, blood compatibility and immunity compatibility, is much lower in immunological rejection response, and can eradicate the potential virus hazard which can not be avoided by animal collagen, causing the security to be improved vastly.

Description

Technical field [0001] The invention relates to a method for preparing a vascular outer layer scaffold material for biodegradable tissue engineering, and belongs to the field of biomedical materials. Background technique [0002] The production of vascular outer stent materials is a very critical link in the field of vascular tissue engineering. As a stent planted with cells in the outer layer of the blood vessel wall, it should have good biocompatibility, biodegradability, good morphology and proper mechanical strength, which is conducive to the adhesion, proliferation and differentiation of fibroblasts, so that the cells are in the stent space. It is distributed and arranged in an orderly manner, forming a tissue with the outer structure and function of the vessel wall with the planting cells and extracellular matrix. [0003] Collagen is the most abundant and widely distributed protein in animals. It is the main structural protein of the body and the main component of supporti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/56A61L27/58A61L27/24A61L27/20
Inventor 范代娣朱晨辉马晓轩米钰骆艳娥马沛惠俊峰朱晓丽李万华梁丽华陈岚
Owner NORTHWEST UNIV(CN)
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