Mycobacterium culture medium, preparation method and method for culture, identification and drug sensitivity test

Abstract

The invention relates to a mycobacterium culture medium, a preparation method and a method for culture, identification and drug sensitivity test. The culture medium contains buffer solution, glycerol, trace element, albumin, glucose, antibiotic and a colour-changing agent and the like. The first fourteenth components are added in distilled water, pH is adjusted, high-pressure sterilization is carried out, methyl thiazolyldiphenyl tetrazolium, albumin-oleic acid-glucose liquid and four types of antibiotics are added and the culture medium is obtained. Specimen after pre-treatment is taken and added into the culture medium for culture, and after the color of ae culture hole is changed, the mycobacterium tuberculosis or nontuberculous mycobacteria and drug sensitivity are judged according to the condition of color change of identification holes and drug-sensitivity testing holes. The invention can obtain the results of mycobacterium culture, identification and drug-sensitivity measurement by only one-time inoculated culture, has simple operation, practicability, easy standardization, high positive rate, fastness, strong practicability, low cost, easy promotion and wide application, and is applicable to mycobacterium culture, identification and drug-sensitivity test in the environments of human bodies, animals and sewage and the like.

Description

technical field [0001] The invention relates to a mycobacterium culture, identification and drug sensitivity culture medium and its preparation method, and a method for simultaneously performing mycobacterium culture, identification and drug sensitivity determination. Background technique [0002] There are more than 100 species of mycobacteria (also known as "mycobacteria"), which are widely distributed in nature, most of which are saprophytic bacteria, and a few are pathogenic bacteria for humans and animals. According to reports at home and abroad, Mycobacterium infection is increasing day by day, Mycobacterium tuberculosis infection epidemic situation and drug resistance are quite serious, and the incidence rate of Mycobacterium tuberculosis infection is also showing an increasing trend year by year. And smear staining examination branch (branch) bacilli need to contain 10 in the specimen 4 / ml branch (mycobacterium) can be detected, the sensitivity is low, and the spec...

AI Technical Summary

Problems solved by technology

Due to the poor practicability of the existing commonly used methods for the identification of mycobacteria (mycobacteria), most laboratories do not carry out the identification of mycobacterium tuberculosis (mycobacteria) and non-tuberculosis mycobacteria

The traditional biochemical reaction identification and drug susceptibility test are cumbersome and time-consuming, requiring 4 to 8 weeks, and there are many influencing factors, such as the amount of bacteria, the age of bacteria, especially the control of the amount of bacteria has no objective standard, and the repeatability is poor

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