Process for preparing catalase and hepatocyte growth-promoting decoction simultaneously by taking animal liver as raw material

A catalase, animal liver technology, applied in the field of separation and extraction of biologically active substances, to achieve the effect of zero emission

Inactive Publication Date: 2009-10-21
江苏省江大绿康生物工程技术研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] It is possible to separate and extract catalase and co-produce hepatocyte growth-promoting liquid, which not only improves the utilization rate of raw materials, but also realizes the value-added and income increase of the enterprise while eliminating waste water and waste residue, forming an economic benefit and resource environment. The new process of win-win benefits has not been reported at home and abroad

Method used

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  • Process for preparing catalase and hepatocyte growth-promoting decoction simultaneously by taking animal liver as raw material
  • Process for preparing catalase and hepatocyte growth-promoting decoction simultaneously by taking animal liver as raw material
  • Process for preparing catalase and hepatocyte growth-promoting decoction simultaneously by taking animal liver as raw material

Examples

Experimental program
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Effect test

example 1

[0033] 1) Preparation of raw materials:

[0034] Get the qualified 10kg pig liver, remove the envelope and chop it up, wash it repeatedly 2-3 times with sterile distilled water, and grind it into pig liver pulp with a colloid mill;

[0035] 2) Solution soaking:

[0036] Add 40kg of sterile distilled water to the liver slurry, mix well, store in the extraction tank, soak for 6-9 hours at a temperature of 28-32°C, and make the target product autodissolve in the solution;

[0037] 3) Solid-liquid separation:

[0038] Centrifuge to obtain about 46L of liquid and 13kg of solid waste;

[0039] 4) Primary membrane (catalase) separation:

[0040] The obtained liquid was separated and extracted through a 40KDa hollow fiber membrane ultrafiltration membrane, and concentrated according to the process requirements to obtain 18L of catalase liquid with an enzyme activity unit of 53,000U / mL;

[0041] 5) Enzymatic hydrolysis of waste residue:

[0042] Add 15 L of sterile distilled water...

example 2

[0050] After freeze-drying the hepatocyte growth-promoting liquid, take 5g of the powder, and analyze its trace element content and amino acid profile. The results are shown in Table 1 and Table 2 respectively:

[0051] Table 1 Total protein content and composition of vitamins and minerals

[0052]

[0053] The sample has been confirmed by amino acid profile detection, which is rich in 17 kinds of amino acids needed by the human body, and the total amino acid reaches 62.91g / 100g.

[0054] Table 2 Amino Acid Composition

[0055]

[0056]

[0057] It has been confirmed by the atomic absorption method that the sample contains abundant trace elements beneficial to the human body, especially iron, zinc, magnesium, and calcium.

example 3

[0059] Take an appropriate amount of liver cell growth-promoting liquid for animal experiments:

[0060] 1) Establishment of animal model: SD rats, half male and half male, were reared under quiet conditions for one week, fed with pure corn dregs plus cholesterol, and fed with 30% ethanol as beverage. On the first day of the experiment, 40% CCl was subcutaneously injected at 0.5 mL / kg 4 Liquid paraffin solution was injected once every 3 days thereafter, and 2 rats were killed at the end of the 8th week, and ordinary pathological sections were made, and light microscopy showed early liver cirrhosis lesions;

[0061] 2) Treatment:

[0062] The 8 rats were randomly divided into 2 groups, one group was fed with high-concentration hepatocyte growth-promoting solution mixed into the feed, and the other group was fed as usual. After 90 days of feeding, ordinary pathological sections were made, and the light microscope showed that the treatment group was better than the control group...

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Abstract

The invention belongs to the technical field of separation and extraction of bioactivator, in particular relating to a process for extracting hepatocyte growth-promoting decoction from waste water and waste residues after extraction of catalase. The process takes animal liver as raw material to prepare catalase by homogenate, solution soaking, solid-liquid separation and primary membrane separation; waste residues merge with primary membrane penetrating liquid after being hydrolyzed by enzyme process and removed by filtering, and suffers from secondary membrane separation and cryoconcentration to obtain the hepatocyte growth-promoting decoction. The decoction can prepare medicine of solution of hepatocyte growth-promoting factors after being purified, or prepare functional food with function of protecting liver after being cold-dried. Taking pork liver for an example, 1kg pork liver can be processed to obtain 2L of liquid caralase with enzyme activity unit being 50000U / mL by operating according to the invention, 200mL of hepatocyte growth-promoting decoction is obtained simultaneously with index of stimulation being 2.3 and total amino acid being 62.91g / 100g.

Description

technical field [0001] The invention relates to a process for simultaneously preparing catalase and liver cell growth-promoting liquid by using animal liver as a raw material, and belongs to the technical field of separation and extraction of biologically active substances. Background technique [0002] Catalase (CAT) is an efficient, safe, environmentally friendly and energy-saving biocatalyst that can rapidly decompose hydrogen peroxide into water and oxygen, and has a wide range of uses in textile, food and other industries. There are three main sources of catalase currently available on the market: it is extracted and purified from the liver of pigs, cattle and other animals; And get. After 2004, the key technology of extracting and producing catalase from the liver of pigs, cattle and other animals in China has made great breakthroughs. Because of the cost and price advantages, it has developed rapidly. At present, the catalase produced by this method occupies most o...

Claims

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Application Information

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IPC IPC(8): C12N9/08C12P21/06
Inventor 吴保承杨春霞汤鲁宏张栋冯志强
Owner 江苏省江大绿康生物工程技术研究有限公司
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