Lactobacillus health-care capsule and preparation method thereof
A technology of health-care capsules and lactic acid bacteria, which is applied in the field of lactic acid bacteria, can solve the problems that the fermentation and production process of active lactic acid bacteria cannot be achieved, and the application effect cannot be achieved, so as to achieve good health care effects, long storage time, and good health care functions.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] A preparation method of lactic acid bacteria health-care capsules is as follows:
[0040] The first step, strain selection:
[0041] Containing the bacterial classification that adopts in the present invention is the lactic acid bacterium that China Agricultural Microorganism Strain Preservation Management Center buys, and a kind of is Streptococcus thermophilus (CACCC 6222) has health-care effect and growth performance is very good; Another kind is a strain milk Streptococcus lactis (ACCC10653), both strains are screened from the human intestinal tract, the bacteria can produce good flavor after fermentation, and produce growth factors that promote the growth of lactic acid bacteria, both strains are preserved by our company;
[0042] Streptococcus thermophilus and Streptococcus lactis were inoculated in the eggplant bottle of MRS lactic acid bacteria medium at a ratio of 1.5:1 for culture, and the culture medium was optimized by orthogonal test method for the two stra...
Embodiment 2
[0053] 1. Production strain preparation:
[0054] Step 1. Strain selection: inoculate Streptococcus thermophilus and Streptococcus lactis in the eggplant bottle of MRS lactic acid bacteria medium at a ratio of 2:1, and optimize the medium for the two strains by orthogonal test , select the best medium for liquid culture, and the culture medium is inoculated on the eggplant bottle of MRS lactic acid bacteria medium. All the other are with embodiment 1;
[0055] Step 2. Seed production: Pour the preserved strain powder into 10% skimmed milk at a ratio of 4:100 to activate and cultivate for 18 hours. The temperature is 37°C, and it is used as a seed for production after being made;
[0056] The third step. Compound fermentation:
[0057] a, sterilization: with embodiment 1.
[0058] b. Fermentation: Inoculate the activated strain in step 2 into the culture medium of the fermenter at a ratio of 4:100, cultivate at a temperature of 38° C. for 20 hours, and have a CO2 concentrat...
Embodiment 3
[0064] The first step. Production strain preparation:
[0065] 1. Strain selection and breeding: with embodiment 1.
[0066] Step 2. Seed production: Pour the preserved bacterial strain powder into 15% skim milk for activation and cultivation for 30 hours at a ratio of 3:100 at a temperature of 28° C. After being prepared, it is used as a seed for production;
[0067] The third step. Compound fermentation:
[0068] a. Sterilization: with embodiment 1.
[0069] B. Fermentation: Inoculate the activated bacterial classification in the step 2 into the culture medium of the fermenter in a ratio of 3: 100, 28° C. of culture temperature, 24 hours of time, CO Concentration is 9%, when the bacteria are in the logarithmic growth terminal phase When the fermentation is over, the number of viable bacteria in the fermentation broth should reach 3×10 9 ~8×10 9 cfu / g;
[0070] The basic substance of the culture medium is 15 parts of skim milk, in addition, different nitrogen sources and...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More