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Method for synthesizing Sudan II complete antigen

A technology of complete antigen and synthesis method, applied in the fields of peptide preparation, chemical instruments and methods, animal/human proteins, etc., can solve the problems of high cost, can not meet the requirements of fast, convenient and accurate, and meet the needs of research , the effect of simple synthesis steps

Inactive Publication Date: 2009-11-25
JIANGNAN UNIV
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Problems solved by technology

In addition, gas chromatography-mass spectrometry (GC-MS), thin-layer chromatography, polarography, capillary electrophoresis, spectrophotometry, etc. can also detect Sudan red samples, and the accuracy of the experiment is high and the repeatability is good. However, these methods Not only does it require expensive instruments and equipment, professional operators, but also has relatively high requirements for inspection materials, and further sample pretreatment is required, which can no longer meet the requirements of modern detection for speed, convenience, and accuracy.

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  • Method for synthesizing Sudan II complete antigen

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Embodiment 1

[0023] (1) Synthesis of Sudan Red II-hemisuccinate artificial hapten:

[0024] Weigh 0.1 mol of Sudan Red II in a 50 mL round bottom flask, dissolve in 5 mL of pyridine, stir to dissolve, add 0.1 mol of succinic anhydride, and stir at 90°C for 24 hours. After the reaction is complete, remove pyridine by rotary evaporation. Cool the reaction solution to 4°C in advance, then transfer it to 20 mL of ice-distilled water with 2 mL of concentrated hydrochloric acid and let it stand overnight, wash the precipitate with distilled water to pH 6.0, and dry it in vacuum to obtain crude Sudan II-hemisuccinate artificial hapten products.

[0025] (2) Preparation of Sudan Red II Complete Antigen:

[0026] Preparation of liquid A: Weigh 0.1mmol of hapten in a 20mL beaker, add 2mL of dimethylformamide to dissolve, add 0.1mmoL of N,N'-dicyclohexylcarbimide, 0.1mmoL of N-hydroxysuccinyl Imine, stirred at room temperature for 60 minutes, stored at 4°C, this solution is A solution.

[0027] Pr...

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Abstract

The invention discloses a method for synthesizing Sudan II complete antigen, and belongs to the technical field of biochemical engineering. The method comprises the following steps: taking Sudan II as a raw material, and performing condensation reaction of Sudan II and succinic anhydride under the pyridine condition to generate Sudan II-hemisuccinic anhydride; making carboxyl on the Sudan II-hemisuccinic anhydride combine with bovine serum albumin BSA by an activation ester method to prepare the Sudan II complete antigen; and measuring the coupling ratio of a conjugate by a spectrophotometry. The method successfully synthesizes the complete antigen of Sudan II, has simple and effective synthesis steps, can be fully used in immunoassay, provides necessary artificial antigen for future research of people, and can meet the requirement of national Sudan II research.

Description

technical field [0001] The invention relates to a method for synthesizing the complete antigen of Sudan Red II, which belongs to the technical field of biochemical industry. Background technique [0002] Sudan red mainly includes Sudan red I, II, III and IV, wherein Sudan red II can produce dimethylaniline (2,4-xylidine) and 1-amino-2-naphthol in vivo metabolism, ARC will Sudan red II and Its metabolite 2,4-dimethylaniline (2,4-xylidine) is listed as three types of carcinogens. Toxicological studies have shown that Sudan Red is mutagenic and carcinogenic. Once ingested into the human body, the oil-soluble Sudan red dye is easily dissolved in the body tissue. Since it is not water-soluble, it will not be excreted in the urine during digestion and circulation. If it is ingested in large doses for a long time, it will accumulate in the body and cause damage to the human body or cause cancer due to gene mutation. Due to the possible carcinogenic properties of Sudan Red, many ...

Claims

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Application Information

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IPC IPC(8): C07K14/765C07K1/34C07K1/113
Inventor 胥传来林菲宋珊珊
Owner JIANGNAN UNIV
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