Method for producing genistein protein powder by using natto bacilli to ferment soybean meal

A technology of Bacillus natto and genistein, applied in the field of microbial fermentation, can solve the problems of low yield of genistein, many by-products, unfavorable industrialization, etc., and achieves the effect of improving physiological activity function and avoiding substrate inhibition.

Inactive Publication Date: 2010-01-06
天津实发中科百奥工业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] One is to use the solvent extraction method; 95% of the soybean isoflavones obtained by this method are still in the bound form, and the content of free aglycone is very small, that is to say, the yield of genistein is extremely low, and it is difficult to regulate various aspects of the human body. The role of physiological function
[0006] The second is to use acid hydrolysis; acid hydrolysis uses strong acid, which is unfavorable to industrialization, and there are many by-products, which pose safety hazards to human life safety and the environment
[0009] At present, the process of producing genistein by using microorganisms is complica

Method used

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Examples

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Example Embodiment

[0023] Example 1

[0024] (1) Domestication of fermentation strains: Bacillus natto, which can produce high β-glucosidase, was inoculated into a 2% (w / v) soybean soybean meal fermentation medium, and after the culture matured, the fermentation broth was diluted to 10 -5 ~10 -8 Then, separate the plates and streak a single colony; for the obtained strains, the enzyme activity of β-glucosidase was measured by the DNS method, and a strain with relatively high enzyme production activity was compared and screened; the selected strains with high enzyme production activity were selected, Then transfer it to a fermentation medium containing 4% (w / v) soybean meal, and after the cultivation matures, take the fermentation broth to dilute and draw the plate screen; the screening method is the same as above; the selected strains with high enzyme production activity are Continue domestication and screening in the fermentation medium containing 6% (w / v) soybean meal to obtain a natto strain that...

Example Embodiment

[0035] Example 2

[0036] (1) Domestication of fermentation strains: Bacillus natto, which can produce high β-glucosidase, was inoculated into a 2% (w / v) soybean soybean meal fermentation medium. After the culture matured, the fermentation broth was diluted to 10 -5 ~10 -8 Then, separate the plates and streak a single colony; for the obtained strains, the enzyme activity of β-glucosidase was measured by the DNS method, and a strain with relatively high enzyme production activity was compared and screened; the selected strains with high enzyme production activity were selected, Then transfer it to a fermentation medium containing 4% (w / v) soybean and soybean meal, and after culturing mature, take the fermentation broth to dilute and draw a plate screen; the screening method is the same as above; and so on, step by step domestication, respectively The strain is domesticated in the fermentation medium of 6% (w / v) and 8% (w / v) soybean meal, and finally the fermentation medium can be a...

Example Embodiment

[0042] Example 3

[0043] (1) Domestication of fermentation strains: Bacillus natto, which can produce high β-glucosidase, was inoculated into a 2% (w / v) soybean soybean meal fermentation medium. After the culture matured, the fermentation broth was diluted to 10 -5 ~10 -8 Then, separate the plates and streak a single colony; for the obtained strains, the enzyme activity of β-glucosidase was measured by the DNS method, and a strain with relatively high enzyme production activity was compared and screened; the selected strains with high enzyme production activity were selected, Then transfer it to a fermentation medium containing 4% (w / v) soybean and soybean meal, and after culturing mature, take the fermentation broth to dilute and draw a plate screen; the screening method is the same as above; and so on, step by step domestication, respectively The strain is domesticated in the fermentation medium of 6% (w / v), 8% (w / v), 10% (w / v) soybean and soybean meal, and finally obtained can...

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Abstract

The invention relates to a method for producing genistein protein powder by using natto bacilli to ferment soybean meal. The method comprises the following steps: (1) acclimating a fermentation strain: obtaining a natto strain which adapts to soybean meal fermentation culture media of 2 percent, 4 percent, 6 percent, 8 percent and 10 percent (w/v) and generates high beta-glucosidase activity; (2) preparing fermentation seeds: inoculating and culturing natto bacilli which have high yield of beta-glucosidase in soybean meal substrate culture media of various concentrations on inclined planes of test tubes; (3) preparing a fermentation culture medium; forming the fermentation culture medium with a pH value of 7.2-7.4 by 1-3 percent (w/v) glucose, 6-12 percent (w/v) defatted soybean meal, 0.05-0.2 percent (w/v) Ca<+>, 0.02-0.1 percent (w/v) Mg<+>; (4) fermenting: inoculating a seed culture medium with an inoculation amount of 4-10 percent (w/v) in the fermentation culture medium, wherein the liquid-containing rate of a fermentation pot is 40-60 percent (w/v), the fermentation temperature is 32-38 DEG C, the pH value is 7.0-7.5, and the fermentation time is 48-72 h; and (5) spraying and drying. The invention has simple operation and remarkable effect.

Description

technical field [0001] The invention relates to microbial fermentation technology, in particular to a method for producing genistein protein powder by fermenting soybean meal with Bacillus natto. Background technique [0002] Soy genistein is one of the main active substances of soybean isoflavones, a natural plant hormone with unique physiological properties. Many research reports and research experiments have shown that genistein is the most active and functional of isoflavones, and is a non-estrogenic compound with weak estrogenic effects. Its physiological effects are: it has pharmacological effects such as increasing bone density, increasing the length of the tibia, forming bone cells, and preventing bone loss. It is a new type of effective drug for the treatment of osteoporosis; Breast cancer and tumors and other diseases can reduce the incidence of cholesterol, heart disease and cancer, and has the functions of anti-oxidation and inhibition of tyrosine protein kinase...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12P17/06C12R1/07
Inventor 晋明芬何雨青王祥河许晓菁
Owner 天津实发中科百奥工业生物技术有限公司
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