Ultrasound-assisted enzymolysis wheat bran method for preparing ferulic acid

A technology of wheat bran and assisted enzymatic hydrolysis, which is applied in fermentation and other directions, can solve the problems of difficult extraction and low extraction rate, and achieve the effects of improving extraction efficiency, shortening extraction time, and reducing pollution

Inactive Publication Date: 2010-01-20
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is not easy to extract, and the ext

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A, at first the wheat bran is pulverized with a pulverizer and sieved to remove impurities such as sand and some starch therein, and accurately weigh 25g of the bran under the 35 mesh sieve in a three-necked bottle;

[0027] B, get the wheat bran obtained in a step, add 70 ℃ of hot water, water bath heating; Wherein the weight ratio of wheat bran and water is 1: 10;

[0028] c. Use α-amylase to enzymatically remove the starch in the wheat bran: first adjust the pH value of the feed liquid obtained in step b to 6.8 with disodium hydrogen phosphate-citric acid buffer solution, then add high-temperature-resistant α-amylase, The amylase action conditions are as follows: the time is 40min; the temperature is: 90°C; the concentration of the enzyme in the reaction system is: 0.5ml / L; the concentration of the substrate is: 100g / L; during this period, the stirring speed is 120r / min. ,; use iodine solution to detect whether the starch is completely hydrolyzed, if it turns blue, c...

Embodiment 2

[0036] a. First, use a pulverizer to crush the wheat bran and sieve it to remove impurities such as sand and some starch, and accurately weigh 25 g of the bran under the 35 mesh sieve in a three-necked bottle.

[0037]b. Take the wheat bran obtained in step (a), add hot water at 70° C., and heat in a water bath; wherein the weight ratio of wheat bran to water is 1:10.

[0038] c. Use α-amylase to enzymatically remove the starch in the wheat bran: first adjust the pH value of the feed liquid obtained in step b to 6.8 with disodium hydrogen phosphate-citric acid buffer solution, then add high-temperature-resistant α-amylase, The amylase action conditions are as follows: the time is 40min; the temperature is: 90°C; the concentration of the enzyme in the reaction system is: 0.5ml / L; the concentration of the substrate is: 100g / L; during this period, the stirring speed is 120r / min. ,; use iodine solution to detect whether the starch is completely hydrolyzed, if it turns blue, contin...

Embodiment 3

[0046] a. First, use a pulverizer to crush the wheat bran and sieve it to remove impurities such as sand and some starch, and accurately weigh 25 g of the bran under the 35 mesh sieve in a three-necked bottle.

[0047] b. Take the wheat bran obtained in step (a), add hot water at 70° C., and heat in a water bath; wherein the weight ratio of wheat bran to water is 1:10.

[0048] c. Use α-amylase to enzymatically remove the starch in the wheat bran: first adjust the pH value of the feed liquid obtained in step b to 6.8 with disodium hydrogen phosphate-citric acid buffer solution, then add high-temperature-resistant α-amylase, The amylase action conditions are as follows: the time is 40min; the temperature is: 90°C; the concentration of the enzyme in the reaction system is: 0.5ml / L; the concentration of the substrate is: 100g / L; during this period, the stirring speed is 120r / min. ,; use iodine solution to detect whether the starch is completely hydrolyzed, if it turns blue, conti...

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PUM

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Abstract

The invention discloses an ultrasound-assisted enzymolysis wheat bran method for preparing ferulic acid, comprising the following steps: a. crushing wheat bran; b. heating wheat bran obtained in step (a) in water bath; c. removing starch in wheat bran by enzymolyzing alpha-amylase; d. removing protein in wheat bran by using alkaline protease; e. centrifugally precipitating suspending liquid, abandoning supernatant, and washing the suspending liquid with distilled water to obtain bran residue; f. drying bran residue; g. carrying out ultrasound treatment on dried wheat bran; h. carrying out enzymatic reaction on bran obtained in step g by xylanase; and i. centrifuging suspending liquid obtained in step (h) by enzymatic reaction, taking supernatant, after washing residue at the lower level, combining supernatant to carry out concentration and vacuum drying to obtain crude ferulic acid; dissolving the crude product in anhydrous ethanol, centrifuging to remove precipitation and obtaining transparent faint yellow supernatant, and then concentrating and drying to obtain ferulic acid crystallization with higher purity.

Description

technical field [0001] The invention relates to the technical fields of development and comprehensive utilization of agricultural by-products, production of functional food additives, pharmaceutical and cosmetic additives, etc., and in particular relates to a method for preparing ferulic acid by using ultrasonic-assisted enzymatic hydrolysis of wheat bran. Background technique [0002] Ferulic acid is a phenolic acid widely present in plant cell wall substances. In wheat bran, it is mainly cross-linked with polysaccharides and lignin through ester bonds and ether bonds, or self-esterified into diferulic acid. . It has cis and trans structures, the cis form is a yellow oil, and the trans form is a white to slightly yellow crystal. Its molecular weight is 194.19, and its melting point is 174°C. It is slightly soluble in cold water, soluble in hot water, easily soluble in ethanol, methanol, and acetone, and hardly soluble in petroleum ether, benzene, etc. Generally refers to ...

Claims

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Application Information

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IPC IPC(8): C12P7/42
Inventor 刘国琴何粉霞李琳
Owner HENAN UNIVERSITY OF TECHNOLOGY
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