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Method for preparing rapeseed peptide by mixed-strains liquid fermentation

A technology for liquid fermentation and rapeseed, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem that the soluble nitrogen content and biological activity need to be further improved, it is difficult to form large-scale industrial production, and the protease activity is affected. problem, to achieve the effect of being conducive to environmental protection, low price, and increasing the content of soluble nitrogen

Inactive Publication Date: 2010-02-10
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses rapeseed protein isolate as raw material, and the amount of enzyme preparation used in production is large and expensive, resulting in high production costs, and because the anti-nutritional factors in rapeseed meal or rapeseed protein isolate cannot be completely removed, the activity of protease is affected. , the degree of hydrolysis is not high, it is difficult to form a large-scale industrial production
[0004] CN200810243461.6 and CN200810106919.3 disclose methods for modifying rapeseed meal by biological fermentation methods. Although harmful substances such as glucosinolate in rapeseed protein are removed, rapeseed peptide or products containing rapeseed peptide are obtained, but The soluble nitrogen content and biological activity of the product still need to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Preparation of liquid fermentation medium

[0021] Nutrient solution composition: glucose 5g, KH 2 PO 4 2g, 1000mL, pH 6.5. Crush the ordinary rapeseed meal to a particle size of about 100 μm, mix it evenly with the rapeseed meal with a nutrient solution at a solid-to-liquid ratio of 1:30 g / mL, and sterilize at 121°C for 30 minutes to obtain a liquid fermentation medium for fermentation inside the tank.

[0022] (2) Liquid fermentation

[0023] ①Preparation of seed solution of Mucor radiata Yazhi:

[0024] Slant medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0025] Plate expansion medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0026] Inoculate the spores of Mucor actinosa on the slant medium and incubate at 28°C for 4 days, then inoculate on the flat plate and carry out expansion culture at 28°C. After 4 days, wash the spores with sterile water, transfer them to the Erlenmeyer flask with glass beads, and shake Break up the sp...

Embodiment 2

[0043] The difference from Example 1 is that (4) purification is carried out as follows:

[0044] Adjust the pH of the rapeseed peptide crude extract to 7.0, add 10g / L activated carbon, decolorize and debitterize at 50°C for 1.5h, then centrifuge at a high speed (relative centrifugal force 10000g) to remove impurities, and the supernatant is passed through a desalting column (HiPrep TM 26 / 10, flow rate 30mL / min) after desalination, spray-dry the rapeseed peptide crude extract to obtain rapeseed peptide, its protein content (dry basis, N × 6.25) is 88%, soluble nitrogen content is 94%, molecular weight is 1000Da The peptide content of ~5000Da is 65%, and the glucosinolate content is 8 μmoL / g.

Embodiment 3

[0046] (1) Preparation of liquid fermentation medium

[0047] Nutrient solution composition: glucose 5g, KH 2 PO 4 2g, 1000mL, pH 6.0. Crush the ordinary rapeseed meal to a particle size of about 100 μm, mix the rapeseed meal with nutrient solution at a solid-to-liquid ratio of 1:15 g / mL, and sterilize at 121°C for 30 minutes to obtain a liquid fermentation medium for fermentation inside the tank.

[0048] (2) Liquid fermentation

[0049] ①Preparation of seed solution of Mucor radiata Yazhi:

[0050] Slant medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0051] Plate expansion medium: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0052] Inoculate the spores of Mucor actinosa on the slant medium and incubate at 28°C for 4 days, then inoculate on the flat plate and carry out expansion culture at 28°C. After 4 days, wash the spores with sterile water, transfer them to the Erlenmeyer flask with glass beads, and shake Break up the spores, filter the ...

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Abstract

The invention relates to a method for preparing rapeseed peptide by mixed-strains liquid fermentation. The functions of hydrolyzing protein and degrading glucosinolate of microorganisms are given fullplay, and the content of soluble nitrogen and the bioactivity of the product can be effectively increased. The method for preparing the rapeseed peptide by mixed-strains liquid fermentation includesthe following steps: (1) powder of rapeseed dregs is mixed with nutrient solution to prepare a liquid fermentation culture medium; (2) Actinomucor elegans and Aspergillus usamii are led into the liquid fermentation culture medium to be processed by liquid fermentation; (3) the fermented liquid culture medium is processed by solid-liquid separation to obtain the crude extract of rapeseed peptide; and (4) the crude extract is purified to obtain the rapeseed peptide. The invention has the advantages of simple preparation process and low cost; and during the mixed fermentation of different microorganisms, various metabolites and enzymes are generated, restriction enzyme cutting sites are different, and the functions of hydrolyzing the protein and degrading the glucosinolate of the microorganisms are given full play, thereby effectively degrading toxic substances in seeds of the rapeseed dregs, hydrolyzing the protein, and increasing the content of soluble nitrogen, the bioactivity and theyield of rapeseed peptide of the product.

Description

technical field [0001] The invention relates to a method for preparing rapeseed peptide by mixed bacteria liquid state fermentation. Background technique [0002] my country is the country with the largest rapeseed production in the world, with an annual output of nearly 12 million tons. Rapeseed meal is the main by-product of rapeseed oil processing, accounting for 65% of the total rapeseed, which contains 35% to 42% protein. The amino acid composition of rapeseed protein is reasonable, the content of lysine, cystine and methionine is relatively high, which is similar to the amino acid composition model recommended by WHO / FAO, and its nutritional value is equal to or better than that of animal protein. It is an excellent vegetable protein. However, due to the existence of some anti-nutritional factors in rapeseed meal, such as glucosinolates, phytic acid, polyphenols, etc., it is difficult to remove them during processing, so rapeseed meal can only be used in the feed or fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P21/06C12R1/785C12R1/66
Inventor 王立峰鞠兴荣袁建何荣金晶
Owner NANJING UNIV OF FINANCE & ECONOMICS
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