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Method for improving fermentation speed of starchy raw material alcohol and liquor yield by using xylanase

A technology of starchy raw materials and xylanase, which is applied in the field of wine making, can solve the problems of low yield of raw materials, high viscosity of fermented mash, and slow fermentation speed, and achieve the effects of increasing fermentation speed, increasing alcohol concentration, and reducing viscosity

Inactive Publication Date: 2010-03-17
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of high viscosity of fermented mash, slow fermentation speed and low yield of raw materials in the above-mentioned existing alcoholic fermentation method, and to develop a cheap and easy-to-use alcoholic fermentation accelerator to reduce the high viscosity of fermented mash The impact on heat and mass transfer effect, and the barrier effect of hemicellulose on the contact between starchy raw materials and liquefaction glucoamylase, thus providing a method of using xylanase to improve the alcoholic fermentation speed of starchy raw materials and the yield of raw materials

Method used

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  • Method for improving fermentation speed of starchy raw material alcohol and liquor yield by using xylanase
  • Method for improving fermentation speed of starchy raw material alcohol and liquor yield by using xylanase
  • Method for improving fermentation speed of starchy raw material alcohol and liquor yield by using xylanase

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Effect test

Embodiment 1

[0021] Embodiment 1: the influence of culture medium water ratio

[0022] For corn raw materials, the amount of xylanase added is 150U / g, the pH value is the natural pH, and xylanase in the addition group is added before inoculation. Adjust the water mass ratio of each group of culture medium to 1:2.2, 1:2.3, 1:2.4, 1:2.5, 1:2.6, 1:2.7, 1:3.0 for the test (other unlisted conditions and processes can refer to In the above embodiment). Each bottle was inoculated with 10% of AY-15 yeast culture seeds, and cultured in a 30°C incubator for 72 hours, and three parallel experiments were carried out for each group of material-water ratio. CO was measured every 12 hours of fermentation 2 weightlessness. After the fermentation, the alcohol concentration and residual sugar were measured respectively, and the yield of ethanol to raw materials was calculated. The experimental results are shown in Table 1. From the results, the wine yield of raw materials is the highest when the ratio o...

Embodiment 2

[0026] Embodiment 2: the influence of xylanase addition amount

[0027] Corn raw materials, the ratio of medium to water is 1.:2.5, the pH value is the natural pH, xylanase is added during the saccharification process, and the amount of xylanase added to each group of medium is 50U / g, 130U / g, respectively. 150U / g, 170U / g, 190U / g raw materials and no xylanase were used as controls. Each bottle was inoculated with 10% of AY-15 yeast cultured seeds, placed in a 30°C incubator for static culture for 72 hours, and three parallel experiments were performed on the amount added in each group. CO was measured every 12 hours of fermentation 2 weightlessness. After the fermentation, the alcohol concentration and residual sugar were measured respectively, and the yield of ethanol to the raw materials was calculated. The experimental results are shown in Table 2. Compared with the control group, after adding different amounts of xylanase, the alcohol content of fermented mash increased ...

Embodiment 3

[0030] Embodiment 3: Add the impact of xylanase on fermentation cycle

[0031] For corn raw materials, the amount of xylanase added is 170U / g, the pH value is the natural pH, the ratio of material to water is 1:2.5, and the xylanase in the added group is added 6 hours after fermentation. Each bottle was inoculated with 10% of AY-15 yeast cultured seeds, cultured in a 30°C incubator, and five time periods of 24h, 36h, 48h, 60h, and 72h were selected to measure the alcohol content of the fermented mash of the control group and the added group. Three parallel experiments were done in each group, and the experimental results are shown in figure 1 . It can be known by comparison that in the whole fermentation process, the fermentation efficiency of the addition group is obviously faster than that of the control group. When the fermentation time was 60h, the alcohol content reached the maximum value, and then tended to be parallel. Compared with the control group, the fermentation...

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Abstract

The invention relates to a method for improving the fermentation speed of starchy raw material alcohol and the liquor yield by using xylanase. The xylanase is applied to the fermentation production ofstarchy raw material alcohol; on one hand, the xylanase can damage the plant cell wall by degrading hemicellulose in the raw material to fully release the cellular content, and the cellular content is fully contacted with hydrolase such as amylase, diastatic enzyme and the like to improve the diastatic fermentation effect; on the other hand, the degradation effect of the xylanase can decrease theviscosity of fermenting mash, boost the liquefied and sacchrified effects and improve the heat transfer and mass transfer effects in the fermentation process so as to improve the fermentation speed of alcohol and the liquor yield of the raw material. Experiment results show that the fermentation period can be shortened by 8-12h, and the liquor yield can be improved by 1.4-3.0% with respect to theraw material by adding 50-190U / g of xylanase in the starchy raw material alcohol. The method has the advantages that the process is simple and effective, the method is suitable for various starchy raw material alcohol fermentation industries, and the alcohol production cost can be reduced.

Description

【Technical field】: [0001] The invention belongs to the technical field of brewing, and relates to a method for increasing the alcoholic fermentation speed of starchy raw materials and the liquor yield of raw materials by adding xylanase. 【Background technique】: [0002] In the brewing industry, starchy raw materials are surrounded by a certain amount of lignocellulose, and lignocellulose is formed by embedding cellulose filaments in a matrix of macromolecular proteins, pectin, lignin and hemicellulose. Among them, the dry weight of the two main components cellulose and hemicellulose accounts for 45% and 25% respectively. Hemicellulose with xylan as the main component not only hinders the degradation of cellulose molecules, but also reduces the utilization rate of starchy raw materials. On the other hand, xylan (especially water-soluble xylan) exists in colloidal form after being dissolved in water, which will increase the viscosity of fermented mash, and a high-viscosity sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12R1/645
CPCY02E50/17Y02E50/10
Inventor 肖冬光陈叶福郭学武王颖
Owner TIANJIN UNIV OF SCI & TECH
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